Gene description for PANK4
Gene name pantothenate kinase 4
Gene symbol PANK4
Other names/aliases -
Species Homo sapiens
 Database cross references - PANK4
ExoCarta ExoCarta_55229
Vesiclepedia VP_55229
Entrez Gene 55229
HGNC 19366
MIM 606162
UniProt Q9NVE7  
 PANK4 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Hepatocytes 26054723    
Mesenchymal stem cells 36408942    
Neuroblastoma cells 25944692    
Thymus 23844026    
 Gene ontology annotations for PANK4
Molecular Function
    pantothenate kinase activity GO:0004594 IMP
    ATP binding GO:0005524 IEA
    phosphatase activity GO:0016791 EXP
    metal ion binding GO:0046872 IEA
Biological Process
    coenzyme A biosynthetic process GO:0015937 IBA
    pantothenate metabolic process GO:0015939 TAS
Subcellular Localization
    nucleus GO:0005634 IBA
    cytosol GO:0005829 IBA
    cytosol GO:0005829 TAS
 Experiment description of studies that identified PANK4 in sEVs
1
Experiment ID 489
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 237
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocytes
Sample name MIHA
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RNA Sequencing
6
Experiment ID 488
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
7
Experiment ID 224
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25944692    
Organism Homo sapiens
Experiment description Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes
Authors "Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S"
Journal name Oncotarget
Publication year 2015
Sample Neuroblastoma cells
Sample name SH-SY5Y
Isolation/purification methods Differential centrifugation
Ultracentrifugation
OptiPrep density gradient
Flotation density 1.10 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
8
Experiment ID 217
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✘ Negative markers
✔ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors "Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O."
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for PANK4
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 DNAJC7 7266
Proximity Label-MS Homo sapiens
2 LY86  
Affinity Capture-MS Homo sapiens
3 ACTR2 10097
Co-fractionation Homo sapiens
4 C18orf8 29919
Co-fractionation Homo sapiens
5 MAP2K2 5605
Affinity Capture-MS Homo sapiens
6 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
7 NPAS1  
Affinity Capture-MS Homo sapiens
8 FOXA3  
Affinity Capture-MS Homo sapiens
9 TTC26 79989
Affinity Capture-MS Homo sapiens
10 AGBL4  
Affinity Capture-MS Homo sapiens
11 UBE2H 7328
Affinity Capture-MS Homo sapiens
12 AIFM1 9131
Affinity Capture-MS Homo sapiens
13 ZHX1-C8orf76  
Affinity Capture-MS Homo sapiens
14 C9orf78 51759
Affinity Capture-MS Homo sapiens
15 RAB1B 81876
Affinity Capture-MS Homo sapiens
16 TPD52L2 7165
Co-fractionation Homo sapiens
17 MFSD4  
Affinity Capture-MS Homo sapiens
18 PTPN6 5777
Co-fractionation Homo sapiens
19 GZMH  
Affinity Capture-MS Homo sapiens
20 TRIM66  
Affinity Capture-MS Homo sapiens
21 NSUN2 54888
Co-fractionation Homo sapiens
22 CUL3 8452
Affinity Capture-MS Homo sapiens
23 FPR1  
Affinity Capture-MS Homo sapiens
24 EFTUD1 79631
Co-fractionation Homo sapiens
25 EGFR 1956
Affinity Capture-MS Homo sapiens
26 OSGEP 55644
Co-fractionation Homo sapiens
27 TERF2IP 54386
Co-fractionation Homo sapiens
28 KLRB1  
Affinity Capture-MS Homo sapiens
29 SAE1 10055
Co-fractionation Homo sapiens
30 EEF2K  
Co-fractionation Homo sapiens
31 AQP3  
Affinity Capture-MS Homo sapiens
32 UBE3C 9690
Co-fractionation Homo sapiens
33 ANTXR1 84168
Affinity Capture-MS Homo sapiens
34 UFL1 23376
Affinity Capture-MS Homo sapiens
35 RPA3 6119
Proximity Label-MS Homo sapiens
36 PARK2  
Affinity Capture-MS Homo sapiens
37 KIF1BP 26128
Co-fractionation Homo sapiens
38 BAG1 573
Co-fractionation Homo sapiens
39 NTRK1 4914
Affinity Capture-MS Homo sapiens
40 SYNE1 23345
Cross-Linking-MS (XL-MS) Homo sapiens
41 DNAJA1 3301
Proximity Label-MS Homo sapiens
42 BRD1 23774
Affinity Capture-MS Homo sapiens
43 TP53RK 112858
Co-fractionation Homo sapiens
44 CLEC2B  
Affinity Capture-MS Homo sapiens
45 DHFRL1  
Proximity Label-MS Homo sapiens
46 FKBP8 23770
Proximity Label-MS Homo sapiens
47 C9orf72  
Affinity Capture-MS Homo sapiens
48 HAX1  
Proximity Label-MS Homo sapiens
49 PRDX2 7001
Affinity Capture-MS Homo sapiens
50 INF2 64423
Affinity Capture-MS Homo sapiens
51 LYPD4  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
52 RANGAP1 5905
Co-fractionation Homo sapiens
53 UFM1 51569
Co-fractionation Homo sapiens
54 EP300 2033
Affinity Capture-MS Homo sapiens
55 CLDN10  
Affinity Capture-MS Homo sapiens
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