Gene description for NSUN2
Gene name NOP2/Sun RNA methyltransferase family, member 2
Gene symbol NSUN2
Other names/aliases MISU
MRT5
SAKI
TRM4
Species Homo sapiens
 Database cross references - NSUN2
ExoCarta ExoCarta_54888
Entrez Gene 54888
HGNC 25994
MIM 610916
UniProt Q08J23  
 NSUN2 identified in exosomes derived from the following tissue/cell type
Hepatocytes 26054723    
Thymus 23844026    
 Gene ontology annotations for NSUN2
Molecular Function
    tRNA (cytosine-5-)-methyltransferase activity GO:0016428 IDA
    poly(A) RNA binding GO:0044822 IDA
    tRNA binding GO:0000049 IEA
Biological Process
    tRNA methylation GO:0030488 IDA
    mitotic nuclear division GO:0007067 IEA
    cell division GO:0051301 IEA
Subcellular Localization
    nucleolus GO:0005730 IDA
    nucleus GO:0005634 IDA
    spindle GO:0005819 IEA
    cytoplasm GO:0005737 IDA
 Experiment description of studies that identified NSUN2 in exosomes
1
Experiment ID 237
ISEV standards
EM
EV Biophysical techniques
TSG101|Alix|HSC70|GAPDH
EV Cytosolic markers
EV Membrane markers
HSP90B1
EV Negative markers
qNano
EV Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocytes
Sample name MIHA
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RNA Sequencing
2
Experiment ID 217
ISEV standards
EM
EV Biophysical techniques
TSG101
EV Cytosolic markers
CD81|CD9|CD63
EV Membrane markers
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O.
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for NSUN2
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which NSUN2 is involved
No pathways found





Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here