Gene description for DDX49
Gene name DEAD (Asp-Glu-Ala-Asp) box polypeptide 49
Gene symbol DDX49
Other names/aliases R27090_2
Species Homo sapiens
 Database cross references - DDX49
ExoCarta ExoCarta_54555
Vesiclepedia VP_54555
Entrez Gene 54555
HGNC 18684
UniProt Q9Y6V7  
 DDX49 identified in exosomes derived from the following tissue/cell type
Mesenchymal stem cells 36408942    
Thymus 23844026    
 Gene ontology annotations for DDX49
Molecular Function
    DNA clamp loader activity GO:0003689 IEA
    RNA binding GO:0003723 HDA
    RNA helicase activity GO:0003724 IDA
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IEA
    ATP hydrolysis activity GO:0016887 IEA
    cohesin loader activity GO:0061775 IEA
    chromatin extrusion motor activity GO:0140584 IEA
    ATP-dependent H3-H4 histone complex chaperone activity GO:0140665 IEA
    ATP-dependent H2AZ histone chaperone activity GO:0140849 IEA
Biological Process
    chromatin remodeling GO:0006338 IEA
    rRNA processing GO:0006364 IBA
    positive regulation of cell growth GO:0030307 IMP
    regulation of rRNA stability GO:0044357 IMP
    chromatin looping GO:0140588 IEA
Subcellular Localization
    nucleus GO:0005634 IBA
    nucleoplasm GO:0005654 IDA
    nucleoplasm GO:0005654 TAS
 Experiment description of studies that identified DDX49 in exosomes
1
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 217
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|CD81|CD9|CD63
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors "Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O."
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for DDX49
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 CD3EAP  
Proximity Label-MS Homo sapiens
2 HAP1  
Two-hybrid Homo sapiens
3 PSMB2 5690
Two-hybrid Homo sapiens
4 RIT1 6016
Negative Genetic Homo sapiens
5 MECP2 4204
Affinity Capture-MS Homo sapiens
6 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
7 PARP1 142
Proximity Label-MS Homo sapiens
8 ABT1 29777
Affinity Capture-MS Homo sapiens
9 FBXL6  
Affinity Capture-MS Homo sapiens
10 Cebpb  
Affinity Capture-Western Rattus norvegicus
11 RNF19B  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
12 CALM2 805
Affinity Capture-MS Homo sapiens
13 RPA2 6118
Proximity Label-MS Homo sapiens
14 UFL1 23376
Affinity Capture-MS Homo sapiens
15 HIST1H2BG 8339
Proximity Label-MS Homo sapiens
16 NSUN2 54888
Co-fractionation Homo sapiens
17 LMNB1 4001
Affinity Capture-MS Homo sapiens
18 NOP56 10528
Proximity Label-MS Homo sapiens
19 USP36  
Affinity Capture-MS Homo sapiens
20 FEM1A  
Affinity Capture-MS Homo sapiens
21 RPS6 6194
Proximity Label-MS Homo sapiens
22 Sf3a3 75062
Two-hybrid Mus musculus
23 CALM1 801
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
24 PARK2  
Affinity Capture-MS Homo sapiens
25 HNRNPA1 3178
Proximity Label-MS Homo sapiens
26 EVL 51466
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
27 NIFK 84365
Proximity Label-MS Homo sapiens
28 APP 351
Reconstituted Complex Homo sapiens
29 LYN 4067
Proximity Label-MS Homo sapiens
30 CENPA  
Proximity Label-MS Homo sapiens
31 APEX1 328
Proximity Label-MS Homo sapiens
32 PNO1 56902
Co-fractionation Homo sapiens
33 DDX23 9416
Proximity Label-MS Homo sapiens
34 AKR7L 246181
Affinity Capture-MS Homo sapiens
35 CTCFL  
Affinity Capture-MS Homo sapiens
36 RPS24 6229
Proximity Label-MS Homo sapiens
37 STX1A 6804
Two-hybrid Homo sapiens
38 RPA3 6119
Proximity Label-MS Homo sapiens
39 RPL31 6160
Proximity Label-MS Homo sapiens
40 CIT 11113
Affinity Capture-MS Homo sapiens
41 DDRGK1 65992
Affinity Capture-MS Homo sapiens
42 Shcbp1 20419
Affinity Capture-MS Mus musculus
43 KRAS 3845
Negative Genetic Homo sapiens
44 CALM3 808
Affinity Capture-MS Homo sapiens
45 KIAA0408  
Affinity Capture-MS Homo sapiens
46 CEBPA  
Protein-peptide Homo sapiens
47 PAGE1  
Affinity Capture-MS Homo sapiens
48 OTX2  
Affinity Capture-MS Homo sapiens
49 ZBTB44  
Affinity Capture-MS Homo sapiens
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