Gene description for GTF2E1
Gene name general transcription factor IIE, polypeptide 1, alpha 56kDa
Gene symbol GTF2E1
Other names/aliases FE
TF2E1
TFIIE-A
Species Homo sapiens
 Database cross references - GTF2E1
ExoCarta ExoCarta_2960
Vesiclepedia VP_2960
Entrez Gene 2960
HGNC 4650
MIM 189962
UniProt P29083  
 GTF2E1 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
 Gene ontology annotations for GTF2E1
Molecular Function
    sequence-specific DNA binding GO:0043565 IEA
    metal ion binding GO:0046872 IEA
    protein binding GO:0005515 IPI
Biological Process
    transcription elongation from RNA polymerase II promoter GO:0006368 TAS
    viral process GO:0016032 TAS
    regulation of transcription, DNA-templated GO:0006355 IEA
    gene expression GO:0010467 TAS
    transcription from RNA polymerase II promoter GO:0006366 TAS
    transcription initiation from RNA polymerase II promoter GO:0006367 TAS
Subcellular Localization
    intermediate filament cytoskeleton GO:0045111 IDA
    nucleoplasm GO:0005654 TAS
 Experiment description of studies that identified GTF2E1 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for GTF2E1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 AES 166
Reconstituted Complex Homo sapiens
2 GTF2A1 2957
Reconstituted Complex Homo sapiens
3 TBP  
Invitro Homo sapiens
Reconstituted Complex Homo sapiens
Co-purification Homo sapiens
Reconstituted Complex Homo sapiens
4 GTF2H4 2968
Invitro Homo sapiens
Reconstituted Complex Homo sapiens
Co-purification Homo sapiens
5 GTF2F1 2962
Co-purification Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
6 ERCC3  
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
7 SURB7  
Affinity Capture-Western Homo sapiens
Co-purification Homo sapiens
8 BRCA1 672
Co-purification Homo sapiens
9 TAF11  
Reconstituted Complex Homo sapiens
Co-purification Homo sapiens
10 TCEA1 6917
Invitro Homo sapiens
11 GTF2H1 2965
Invitro Homo sapiens
12 TIF1  
Invitro Homo sapiens
13 GTF2E2  
Affinity Capture-Western Homo sapiens
Invitro Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
14 TAF6 6878
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
15 SND1 27044
Reconstituted Complex Homo sapiens
16 GTF2B 2959
Invitro Homo sapiens
Reconstituted Complex Homo sapiens
Co-purification Homo sapiens
17 TAF1  
Reconstituted Complex Homo sapiens
18 POLR2A 5430
Co-purification Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
19 GTF2F2 2963
Invitro Homo sapiens
Reconstituted Complex Homo sapiens
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