Gene description for ATF7IP
Gene name activating transcription factor 7 interacting protein
Gene symbol ATF7IP
Other names/aliases AM
ATF-IP
MCAF
MCAF1
p621
Species Homo sapiens
 Database cross references - ATF7IP
ExoCarta ExoCarta_55729
Vesiclepedia VP_55729
Entrez Gene 55729
HGNC 20092
MIM 613644
UniProt Q6VMQ6  
 ATF7IP identified in sEVs derived from the following tissue/cell type
Hepatocellular carcinoma cells 26054723    
 Gene ontology annotations for ATF7IP
Molecular Function
    transcription coregulator activity GO:0003712 IBA
    transcription coregulator activity GO:0003712 IDA
    transcription corepressor activity GO:0003714 IEA
    protein binding GO:0005515 IPI
    ATP hydrolysis activity GO:0016887 IEA
Biological Process
    negative regulation of transcription by RNA polymerase II GO:0000122 IEA
    DNA methylation-dependent constitutive heterochromatin formation GO:0006346 IMP
    regulation of DNA-templated transcription GO:0006355 IBA
    negative regulation of DNA-templated transcription GO:0045892 IDA
    positive regulation of DNA-templated transcription GO:0045893 IDA
Subcellular Localization
    nucleus GO:0005634 IBA
    nucleus GO:0005634 IDA
    nucleus GO:0005634 IMP
    nucleoplasm GO:0005654 IDA
    nucleoplasm GO:0005654 TAS
    transcription regulator complex GO:0005667 IBA
    cytosol GO:0005829 IDA
    nuclear body GO:0016604 IDA
 Experiment description of studies that identified ATF7IP in sEVs
1
Experiment ID 235
MISEV standards
EM
Biophysical techniques
TSG101|Alix|HSC70|GAPDH
Enriched markers
HSP90B1
Negative markers
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
 Protein-protein interactions for ATF7IP
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 CD3EAP  
Proximity Label-MS Homo sapiens
2 ERCC3  
Affinity Capture-Western Homo sapiens
3 DISC1 27185
Two-hybrid Homo sapiens
4 SLX4  
Affinity Capture-MS Homo sapiens
5 ZNF174  
Affinity Capture-MS Homo sapiens
6 CBX1 10951
Affinity Capture-MS Homo sapiens
7 HOXA2  
Proximity Label-MS Homo sapiens
8 HSPB2  
Affinity Capture-MS Homo sapiens
9 SOX5 6660
Proximity Label-MS Homo sapiens
10 MAD2L1 4085
Affinity Capture-MS Homo sapiens
11 YAF2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
12 HMGB1 3146
Two-hybrid Homo sapiens
13 PRR20A  
Affinity Capture-MS Homo sapiens
14 TADA3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
15 SP1  
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
16 EHMT2 10919
Two-hybrid Homo sapiens
17 CRYAA 1409
Affinity Capture-MS Homo sapiens
18 Rbm8a  
Affinity Capture-MS Mus musculus
19 HNF1A  
Proximity Label-MS Homo sapiens
20 KLF3  
Proximity Label-MS Homo sapiens
21 MAPK3 5595
Affinity Capture-MS Homo sapiens
22 RNF4 6047
Affinity Capture-MS Homo sapiens
23 ARL14EP  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
24 HNF4A  
Proximity Label-MS Homo sapiens
25 SMAD4  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
26 VHL  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
27 YY1 7528
Proximity Label-MS Homo sapiens
28 BRLF1  
Two-hybrid
Reconstituted Complex
Affinity Capture-Western
Reconstituted Complex
Affinity Capture-Western
29 RPL8 6132
Co-fractionation Homo sapiens
30 IGHG2 3501
Affinity Capture-MS Homo sapiens
31 PIAS4  
Affinity Capture-MS Homo sapiens
32 CREB1  
Two-hybrid Homo sapiens
33 SETDB1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
34 MTRF1  
Affinity Capture-MS Homo sapiens
35 CBX3 11335
Proximity Label-MS Homo sapiens
36 Cxxc5  
Affinity Capture-MS Mus musculus
37 UBE2I 7329
Two-hybrid Homo sapiens
38 NCAPH 23397
Affinity Capture-MS Homo sapiens
39 PRR20E  
Affinity Capture-MS Homo sapiens
40 SREBF2 6721
Two-hybrid Homo sapiens
41 RPA3 6119
Proximity Label-MS Homo sapiens
42 GTF2E2  
Affinity Capture-Western Homo sapiens
43 KLF8  
Proximity Label-MS Homo sapiens
44 TNNC2  
Affinity Capture-MS Homo sapiens
45 SUMO2 6613
Reconstituted Complex Homo sapiens
46 GTF2E1 2960
Co-fractionation Homo sapiens
Affinity Capture-Western Homo sapiens
47 ERG  
Proximity Label-MS Homo sapiens
48 Setdb1  
Affinity Capture-MS Mus musculus
49 HNF1B  
Proximity Label-MS Homo sapiens
50 SUV39H1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
51 PML 5371
Co-localization Homo sapiens
52 CDY2A  
Affinity Capture-MS Homo sapiens
53 ERCC2 2068
Affinity Capture-Western Homo sapiens
54 MBD1  
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
55 KRT38 8687
Affinity Capture-MS Homo sapiens
56 ATF7IP2 80063
Affinity Capture-MS Homo sapiens
57 POLR2A 5430
Affinity Capture-Western Homo sapiens
58 KLF12  
Proximity Label-MS Homo sapiens
59 RYBP  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
60 DAXX  
Affinity Capture-MS Homo sapiens
61 ELF4 2000
Proximity Label-MS Homo sapiens
View the network image/svg+xml



Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here