Gene description for RNF4
Gene name ring finger protein 4
Gene symbol RNF4
Other names/aliases RES4-26
SLX5
SNURF
Species Homo sapiens
 Database cross references - RNF4
ExoCarta ExoCarta_6047
Vesiclepedia VP_6047
Entrez Gene 6047
HGNC 10067
MIM 602850
UniProt P78317  
 RNF4 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
 Gene ontology annotations for RNF4
Molecular Function
    sequence-specific DNA binding transcription factor activity GO:0003700 TAS
    ubiquitin-protein transferase activity GO:0004842 ISS
    identical protein binding GO:0042802 IPI
    androgen receptor binding GO:0050681 NAS
    ligase activity GO:0016874 IEA
    SUMO polymer binding GO:0032184 ISS
    protein binding GO:0005515 IPI
    zinc ion binding GO:0008270 IEA
    transcription coactivator activity GO:0003713 NAS
    nucleosome binding GO:0031491 ISS
    DNA binding GO:0003677 ISS
    transcription factor binding GO:0008134 IEA
Biological Process
    transcription, DNA-templated GO:0006351 IEA
    protein K48-linked ubiquitination GO:0070936 ISS
    proteasome-mediated ubiquitin-dependent protein catabolic process GO:0043161 IMP
    positive regulation of transcription from RNA polymerase II promoter GO:0045944 IDA
    regulation of kinetochore assembly GO:0090234 IMP
    androgen receptor signaling pathway GO:0030521 NAS
    response to arsenic-containing substance GO:0046685 IDA
    positive regulation of transcription, DNA-templated GO:0045893 ISS
    regulation of spindle assembly GO:0090169 IMP
    protein K6-linked ubiquitination GO:0085020 ISS
    protein autoubiquitination GO:0051865 ISS
    protein K63-linked ubiquitination GO:0070534 ISS
    protein K11-linked ubiquitination GO:0070979 ISS
Subcellular Localization
    nucleus GO:0005634 IDA
    PML body GO:0016605 IDA
    cytoplasm GO:0005737 IDA
 Experiment description of studies that identified RNF4 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for RNF4
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 GSCL  
Invivo Homo sapiens
Invitro Homo sapiens
Two-hybrid Homo sapiens
2 AR 367
Invivo Homo sapiens
Invivo Homo sapiens
Invivo Homo sapiens
Invitro Homo sapiens
Invitro Homo sapiens
Invitro Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
3 TCF20  
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
4 ZNF278  
Affinity Capture-Western Homo sapiens
Invivo Homo sapiens
Invitro Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
View the network image/svg+xml
 Pathways in which RNF4 is involved
PathwayEvidenceSource
Antigen processing: Ubiquitination & Proteasome degradation TAS Reactome





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