Gene description for EIF3J
Gene name eukaryotic translation initiation factor 3, subunit J
Gene symbol EIF3J
Other names/aliases EIF3S1
eIF3-alpha
eIF3-p35
Species Homo sapiens
 Database cross references - EIF3J
ExoCarta ExoCarta_8669
Entrez Gene 8669
HGNC 3270
MIM 603910
UniProt O75822  
 EIF3J identified in exosomes derived from the following tissue/cell type
Squamous carcinoma cells 20124223    
 Gene ontology annotations for EIF3J
Molecular Function
    translation initiation factor activity GO:0003743 IC
    protein binding GO:0005515 IPI
Biological Process
    translational initiation GO:0006413 TAS
    cytoplasmic translation GO:0002181 IBA
    cellular protein metabolic process GO:0044267 TAS
    gene expression GO:0010467 TAS
    translation GO:0006412 TAS
    formation of translation preinitiation complex GO:0001731 IEA
    regulation of translational initiation GO:0006446 IEA
Subcellular Localization
    eukaryotic 48S preinitiation complex GO:0033290 IEA
    cytosol GO:0005829 TAS
    eukaryotic 43S preinitiation complex GO:0016282 IEA
    eukaryotic translation initiation factor 3 complex GO:0005852 IDA
 Experiment description of studies that identified EIF3J in exosomes
1
Experiment ID 191
ISEV standards
✘ EV Biophysical techniques
✔
Alix
EV Cytosolic markers
✔
CD81|CD9
EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20124223    
Organism Homo sapiens
Experiment description Hypoxic tumor cell modulates its microenvironment to enhance angiogenic and metastatic potential by secretion of proteins and exosomes.
Authors Park JE, Tan HS, Datta A, Lai RC, Zhang H, Meng W, Lim SK, Sze SK.
Journal name Mol Cell Proteomics
Publication year 2010
Sample Squamous carcinoma cells
Sample name Squamous carcinoma cell (A431)
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for EIF3J
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 SIAH1  
Invivo Homo sapiens
2 TPD52L1 7164
Two-hybrid Homo sapiens
3 EIF3A 8661
Co-purification Homo sapiens
Invitro Homo sapiens
Invivo Homo sapiens
4 EIF3B 8662
Co-purification Homo sapiens
5 MAGED1  
Two-hybrid Homo sapiens
6 EIF6 3692
Co-purification Homo sapiens
7 GOLM1 51280
Two-hybrid Homo sapiens
8 EIF3D 8664
Invivo Homo sapiens
9 EIF3C 8663
Co-purification Homo sapiens
Invivo Homo sapiens
10 TADA3L  
Two-hybrid Homo sapiens
11 EIF3K 27335
Reconstituted Complex Homo sapiens
View the network image/svg+xml



Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here