Gene description for TPD52L1
Gene name tumor protein D52-like 1
Gene symbol TPD52L1
Other names/aliases D53
hD53
Species Homo sapiens
 Database cross references - TPD52L1
ExoCarta ExoCarta_7164
Vesiclepedia VP_7164
Entrez Gene 7164
HGNC 12006
MIM 604069
UniProt Q16890  
 TPD52L1 identified in exosomes derived from the following tissue/cell type
Hepatocellular carcinoma cells 26054723    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
 Gene ontology annotations for TPD52L1
Molecular Function
    protein heterodimerization activity GO:0046982 IDA
    identical protein binding GO:0042802 IPI
    protein binding GO:0005515 IPI
    protein homodimerization activity GO:0042803 IDA
Biological Process
    regulation of cysteine-type endopeptidase activity involved in apoptotic process GO:0043281 IDA
    positive regulation of MAP kinase activity GO:0043406 IDA
    positive regulation of apoptotic signaling pathway GO:2001235 IDA
    G2/M transition of mitotic cell cycle GO:0000086 IDA
    positive regulation of JNK cascade GO:0046330 IDA
Subcellular Localization
    cytoplasm GO:0005737 IDA
    perinuclear region of cytoplasm GO:0048471 IDA
 Experiment description of studies that identified TPD52L1 in exosomes
1
Experiment ID 236
MISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
EV Enriched markers
✔
HSP90B1
EV Negative markers
✔
qNano
EV Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name MHCC97L
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
2
Experiment ID 834
MISEV standards
✔
EM
EV Biophysical techniques
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
EV Enriched markers
✔
CANX
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
3
Experiment ID 835
MISEV standards
✔
EM
EV Biophysical techniques
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
EV Enriched markers
✔
CANX
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for TPD52L1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 TPD52L1 7164
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
2 C7orf36  
Two-hybrid Homo sapiens
3 GPR135  
Two-hybrid Homo sapiens
4 TPD52 7163
Invitro Homo sapiens
Affinity Capture-MS Homo sapiens
5 MAL2 114569
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
6 EIF3J 8669
Two-hybrid Homo sapiens
7 HDAC1 3065
Two-hybrid Homo sapiens
8 YWHAB 7529
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which TPD52L1 is involved
PathwayEvidenceSource
Golgi Associated Vesicle Biogenesis TAS Reactome





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