Gene description for ISYNA1
Gene name inositol-3-phosphate synthase 1
Gene symbol ISYNA1
Other names/aliases INO1
INOS
IPS
IPS 1
IPS-1
Species Homo sapiens
 Database cross references - ISYNA1
ExoCarta ExoCarta_51477
Vesiclepedia VP_51477
Entrez Gene 51477
HGNC 29821
MIM 611670
UniProt Q9NPH2  
 ISYNA1 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Hepatocytes 26054723    
Mesenchymal stem cells 36408942    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
 Gene ontology annotations for ISYNA1
Molecular Function
    inositol-3-phosphate synthase activity GO:0004512 IBA
    inositol-3-phosphate synthase activity GO:0004512 IGI
    inositol-3-phosphate synthase activity GO:0004512 IMP
    protein binding GO:0005515 IPI
Biological Process
    inositol biosynthetic process GO:0006021 IBA
    inositol biosynthetic process GO:0006021 IEA
    inositol biosynthetic process GO:0006021 IGI
    phospholipid biosynthetic process GO:0008654 IEA
Subcellular Localization
    cytoplasm GO:0005737 IBA
    cytosol GO:0005829 TAS
 Experiment description of studies that identified ISYNA1 in exosomes
1
Experiment ID 489
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 237
MISEV standards
EM
Biophysical techniques
TSG101|Alix|HSC70|GAPDH
Enriched markers
HSP90B1
Negative markers
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocytes
Sample name MIHA
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RNA Sequencing
6
Experiment ID 488
MISEV standards
EM
Biophysical techniques
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
CANX
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
7
Experiment ID 211
MISEV standards
EM
Biophysical techniques
TSG101|Alix|EpCAM|TFRC
Enriched markers
cytochrome c|GOLGA2
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
8
Experiment ID 212
MISEV standards
CEM
Biophysical techniques
TSG101|Alix|EpCAM|TFRC
Enriched markers
Cytochrome C|GOLGA2
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for ISYNA1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 UBE2H 7328
Affinity Capture-MS Homo sapiens
2 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
3 FBXW11  
Affinity Capture-MS Homo sapiens
4 ALDOC 230
Co-fractionation Homo sapiens
5 HNRNPH2 3188
Co-fractionation Homo sapiens
6 PREP 5550
Co-fractionation Homo sapiens
7 NACA2 342538
Co-fractionation Homo sapiens
8 POT1  
Two-hybrid Homo sapiens
9 TRAF4 9618
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
10 PRNP 5621
Affinity Capture-MS Homo sapiens
11 TPD52L1 7164
Co-fractionation Homo sapiens
12 CYB5B 80777
Co-fractionation Homo sapiens
13 RPA2 6118
Proximity Label-MS Homo sapiens
14 EEF2 1938
Co-fractionation Homo sapiens
15 EZR 7430
Co-fractionation Homo sapiens
16 DLD 1738
Co-fractionation Homo sapiens
17 ACAA2 10449
Co-fractionation Homo sapiens
18 CUL3 8452
Affinity Capture-MS Homo sapiens
19 EGFR 1956
Negative Genetic Homo sapiens
20 HSD17B10 3028
Co-fractionation Homo sapiens
21 ATP1B3 483
Cross-Linking-MS (XL-MS) Homo sapiens
22 ACE2 59272
Co-fractionation Homo sapiens
23 UCHL3 7347
Co-fractionation Homo sapiens
24 ECHS1 1892
Co-fractionation Homo sapiens
25 ARHGDIA 396
Co-fractionation Homo sapiens
26 GRSF1 2926
Two-hybrid Homo sapiens
27 PLS1 5357
Co-fractionation Homo sapiens
28 NAA30 122830
Affinity Capture-MS Homo sapiens
29 RDX 5962
Co-fractionation Homo sapiens
30 CDH1 999
Proximity Label-MS Homo sapiens
31 CYB5A 1528
Co-fractionation Homo sapiens
32 RPA3 6119
Proximity Label-MS Homo sapiens
33 VCP 7415
Co-fractionation Homo sapiens
34 NTRK1 4914
Affinity Capture-MS Homo sapiens
35 ERN2  
Co-fractionation Homo sapiens
36 PPP2CA 5515
Two-hybrid Homo sapiens
37 DUSP14 11072
Two-hybrid Homo sapiens
38 GLRX3 10539
Co-fractionation Homo sapiens
39 PSMD9 5715
Co-fractionation Homo sapiens
40 SUMO3 6612
Co-fractionation Homo sapiens
41 MSN 4478
Co-fractionation Homo sapiens
42 ABCE1 6059
Affinity Capture-MS Homo sapiens
43 PLD2 5338
Affinity Capture-MS Homo sapiens
44 PGD 5226
Co-fractionation Homo sapiens
45 CUL4A 8451
Affinity Capture-MS Homo sapiens
46 ALDOA 226
Co-fractionation Homo sapiens
47 PPIB 5479
Co-fractionation Homo sapiens
48 OGT 8473
Reconstituted Complex Homo sapiens
49 C9orf72  
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which ISYNA1 is involved
PathwayEvidenceSource
Inositol phosphate metabolism TAS Reactome
Metabolism TAS Reactome
Synthesis of IP2, IP, and Ins in the cytosol TAS Reactome





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