Gene description for CES2
Gene name carboxylesterase 2
Gene symbol CES2
Other names/aliases CE-2
CES2A1
PCE-2
iCE
Species Homo sapiens
 Database cross references - CES2
ExoCarta ExoCarta_8824
Vesiclepedia VP_8824
Entrez Gene 8824
HGNC 1864
MIM 605278
UniProt O00748  
 CES2 identified in exosomes derived from the following tissue/cell type
Colorectal cancer cells 25890246    
Colorectal cancer cells 25890246    
Hepatocytes 26054723    
 Gene ontology annotations for CES2
Molecular Function
    methylumbelliferyl-acetate deacetylase activity GO:0047374 IEA
    carboxylic ester hydrolase activity GO:0052689 IBA
    carboxylesterase activity GO:0106435 IDA
    carboxylesterase activity GO:0106435 TAS
Biological Process
    prostaglandin metabolic process GO:0006693 IDA
    xenobiotic metabolic process GO:0006805 TAS
    catabolic process GO:0009056 TAS
Subcellular Localization
    endoplasmic reticulum GO:0005783 TAS
    endoplasmic reticulum lumen GO:0005788 TAS
    intracellular membrane-bounded organelle GO:0043231 IBA
 Experiment description of studies that identified CES2 in exosomes
1
Experiment ID 282
MISEV standards
✔
CEM
Biophysical techniques
✔
Alix|TSG101|CD63|CD81|EpCAM
Enriched markers
✘ Negative markers
✔
DLS
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25890246    
Organism Homo sapiens
Experiment description Highly-purified exosomes and shed microvesicles isolated from the human colon cancer cell line LIM1863 by sequential centrifugal ultrafiltration are biochemically and functionally distinct.
Authors "Xu R, Greening DW, Rai A, Ji H, Simpson RJ."
Journal name Methods
Publication year 2015
Sample Colorectal cancer cells
Sample name LIM1863 - Ultracentrifugation - Rep 1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Centrifugal concentration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
2
Experiment ID 283
MISEV standards
✔
CEM
Biophysical techniques
✔
Alix|TSG101|CD63|CD81|EpCAM
Enriched markers
✘ Negative markers
✔
DLS
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25890246    
Organism Homo sapiens
Experiment description Highly-purified exosomes and shed microvesicles isolated from the human colon cancer cell line LIM1863 by sequential centrifugal ultrafiltration are biochemically and functionally distinct.
Authors "Xu R, Greening DW, Rai A, Ji H, Simpson RJ."
Journal name Methods
Publication year 2015
Sample Colorectal cancer cells
Sample name LIM1863 - Ultracentrifugation - Rep 2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Centrifugal concentration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
3
Experiment ID 237
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocytes
Sample name MIHA
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RNA Sequencing
 Protein-protein interactions for CES2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 FNDC5  
Affinity Capture-MS Homo sapiens
2 SNTG1  
Affinity Capture-MS Homo sapiens
3 PKN2 5586
Affinity Capture-MS Homo sapiens
4 STK24 8428
Affinity Capture-MS Homo sapiens
5 THUMPD1 55623
Co-fractionation Homo sapiens
6 SCYL1 57410
Affinity Capture-MS Homo sapiens
7 ANKRD9  
Affinity Capture-MS Homo sapiens
8 CRELD1 78987
Affinity Capture-MS Homo sapiens
9 STIM1 6786
Affinity Capture-MS Homo sapiens
10 RTFDC1  
Affinity Capture-MS Homo sapiens
11 RPRD1A  
Co-fractionation Homo sapiens
12 PNMAL1  
Affinity Capture-MS Homo sapiens
13 STK26 51765
Affinity Capture-MS Homo sapiens
14 KLHDC4 54758
Affinity Capture-MS Homo sapiens
15 PI4K2A 55361
Affinity Capture-MS Homo sapiens
16 BTF3L4 91408
Affinity Capture-MS Homo sapiens
17 APP 351
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
18 PUS7 54517
Affinity Capture-MS Homo sapiens
19 ARHGEF1 9138
Affinity Capture-MS Homo sapiens
20 SERPINF2 5345
Affinity Capture-MS Homo sapiens
21 PPP1R12A 4659
Affinity Capture-MS Homo sapiens
22 EIF3J 8669
Affinity Capture-MS Homo sapiens
23 FYN 2534
Affinity Capture-MS Homo sapiens
24 HIBADH 11112
Affinity Capture-MS Homo sapiens
25 TRIP10 9322
Affinity Capture-MS Homo sapiens
26 SLC22A4 6583
Affinity Capture-MS Homo sapiens
27 CHMP1A 5119
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which CES2 is involved
PathwayEvidenceSource
Aspirin ADME TAS Reactome
Biological oxidations TAS Reactome
Drug ADME TAS Reactome
Metabolism TAS Reactome
Phase I - Functionalization of compounds TAS Reactome





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