Gene description for FYN
Gene name FYN proto-oncogene, Src family tyrosine kinase
Gene symbol FYN
Other names/aliases SLK
SYN
p59-FYN
Species Homo sapiens
 Database cross references - FYN
ExoCarta ExoCarta_2534
Vesiclepedia VP_2534
Entrez Gene 2534
HGNC 4037
MIM 137025
UniProt P06241  
 FYN identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Colorectal cancer cells 34887515    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Melanoma cells 25950383    
Melanoma cells 25950383    
Melanoma cells 25950383    
Melanoma cells 25950383    
Melanoma cells 25950383    
Melanoma cells 25950383    
Melanoma cells 25950383    
Mesenchymal stem cells 36408942    
Neuroblastoma cells 25944692    
Platelets 25332113    
Platelets 25332113    
Platelets 25332113    
Prostate cancer cells 25844599    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
Thymus 23844026    
Urine 22418980    
Urine 22418980    
 Gene ontology annotations for FYN
Molecular Function
    growth factor receptor binding GO:0070851 IPI
    ephrin receptor binding GO:0046875 IPI
    peptide hormone receptor binding GO:0051428 IEA
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IEA
    ion channel binding GO:0044325 IEA
    CD4 receptor binding GO:0042609 IEA
    tubulin binding GO:0015631 IEA
    glycoprotein binding GO:0001948 IPI
    phosphatidylinositol 3-kinase binding GO:0043548 IEA
    G-protein coupled receptor binding GO:0001664 IEA
    CD8 receptor binding GO:0042610 IEA
    non-membrane spanning protein tyrosine kinase activity GO:0004715 NAS
    metal ion binding GO:0046872 IEA
    T cell receptor binding GO:0042608 IEA
    protein tyrosine kinase activity GO:0004713 TAS
Biological Process
    cellular response to platelet-derived growth factor stimulus GO:0036120 IEA
    response to ethanol GO:0045471 IEA
    cell migration GO:0016477 IBA
    positive regulation of protein localization to nucleus GO:1900182 IEA
    T cell costimulation GO:0031295 TAS
    calcium ion transport GO:0006816 NAS
    intracellular signal transduction GO:0035556 TAS
    forebrain development GO:0030900 IEA
    detection of mechanical stimulus involved in sensory perception of pain GO:0050966 IEA
    protein phosphorylation GO:0006468 NAS
    viral process GO:0016032 TAS
    regulation of cell proliferation GO:0042127 IBA
    response to drug GO:0042493 IEA
    regulation of cell shape GO:0008360 IEA
    positive regulation of neuron projection development GO:0010976 IEA
    T cell activation GO:0042110 IBA
    neuron migration GO:0001764 IEA
    stimulatory C-type lectin receptor signaling pathway GO:0002223 TAS
    cell differentiation GO:0030154 IBA
    platelet activation GO:0030168 TAS
    blood coagulation GO:0007596 TAS
    transmembrane receptor protein tyrosine kinase signaling pathway GO:0007169 IBA
    negative regulation of gene expression GO:0010629 IEA
    negative regulation of neuron apoptotic process GO:0043524 IEA
    cellular response to peptide hormone stimulus GO:0071375 IBA
    Fc-epsilon receptor signaling pathway GO:0038095 TAS
    learning GO:0007612 TAS
    activated T cell proliferation GO:0050798 IEA
    fibroblast growth factor receptor signaling pathway GO:0008543 TAS
    negative regulation of extrinsic apoptotic signaling pathway in absence of ligand GO:2001240 IEA
    epidermal growth factor receptor signaling pathway GO:0007173 TAS
    negative regulation of protein catabolic process GO:0042177 IEA
    peptidyl-tyrosine phosphorylation GO:0018108 IMP
    phosphatidylinositol-mediated signaling GO:0048015 TAS
    leukocyte migration GO:0050900 TAS
    Fc-gamma receptor signaling pathway involved in phagocytosis GO:0038096 TAS
    positive regulation of I-kappaB kinase/NF-kappaB signaling GO:0043123 IEA
    innate immune response GO:0045087 TAS
    neurotrophin TRK receptor signaling pathway GO:0048011 TAS
    feeding behavior GO:0007631 TAS
    dendrite morphogenesis GO:0048813 IEA
    regulation of defense response to virus by virus GO:0050690 TAS
    peptidyl-tyrosine autophosphorylation GO:0038083 IBA
    ephrin receptor signaling pathway GO:0048013 TAS
    positive regulation of phosphatidylinositol 3-kinase signaling GO:0014068 IEA
    regulation of apoptotic process GO:0042981 IBA
    vascular endothelial growth factor receptor signaling pathway GO:0048010 TAS
    axon guidance GO:0007411 TAS
    T cell receptor signaling pathway GO:0050852 IDA
Subcellular Localization
    actin filament GO:0005884 IEA
    nucleus GO:0005634 IEA
    plasma membrane GO:0005886 IDA
    postsynaptic density GO:0014069 IEA
    cytosol GO:0005829 TAS
    membrane raft GO:0045121 IDA
    mitochondrion GO:0005739 IEA
    endosome GO:0005768 IDA
    extrinsic component of cytoplasmic side of plasma membrane GO:0031234 IBA
 Experiment description of studies that identified FYN in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 207
MISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|HSP70|FLOT1
EV Enriched markers
✔
VDAC
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ.
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DKO-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
6
Experiment ID 208
MISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|HSP70|FLOT1
EV Enriched markers
✔
VDAC
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ.
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name Dks-8
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
7
Experiment ID 209
MISEV standards
✘ EV Biophysical techniques
✔
TSG101|HSP70|FLOT1
EV Enriched markers
✔
VDAC
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ.
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DLD-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
8
Experiment ID 1203
MISEV standards
✔
EM
EV Biophysical techniques
✔
SDCBP|FLOT1|CD9|CD81|CD63|EPCAM|GAPDH|LAMP1|TFRC|CD151|CD82|LAMP2|RAB35|TSG101|FLOT2|RAB5B|ICAM1|RAB5A
EV Enriched markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 34887515    
Organism Homo sapiens
Experiment description Supermeres are functional extracellular nanoparticles replete with disease biomarkers and therapeutic targets
Authors Zhang Q, Jeppesen DK, Higginbotham JN, Graves-Deal R, Trinh VQ, Ramirez MA, Sohn Y, Neininger AC, Taneja N, McKinley ET, Niitsu H, Cao Z, Evans R, Glass SE, Ray KC, Fissell WH, Hill S, Rose KL, Huh WJ, Washington MK, Ayers GD, Burnette DT, Sharma S, Rome LH, Franklin JL, Lee YA, Liu Q, Coffey RJ.
Journal name Nat Cell Biol
Publication year 2021
Sample Colorectal cancer cells
Sample name DiFi
Isolation/purification methods Differential centrifugation
Filtration
Centrifugal ultrafiltration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
miRNA
Methods used in the study Western blotting
Mass spectrometry
RNA sequencing
9
Experiment ID 363
MISEV standards
✘ EV Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
EV Enriched markers
✔
DCLK1
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M.
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
10
Experiment ID 364
MISEV standards
✘ EV Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
EV Enriched markers
✔
DCLK1
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M.
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
11
Experiment ID 365
MISEV standards
✘ EV Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
EV Enriched markers
✔
DCLK1
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M.
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
12
Experiment ID 254
MISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|FLOT1|CD81
EV Enriched markers
✘ EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name MNT-1
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
13
Experiment ID 255
MISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|FLOT1
EV Enriched markers
✘ EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name G1
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
14
Experiment ID 256
MISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|FLOT1
EV Enriched markers
✘ EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name 501mel
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
15
Experiment ID 257
MISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|FLOT1
EV Enriched markers
✘ EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name Daju
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
16
Experiment ID 258
MISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|FLOT1|CD81
EV Enriched markers
✘ EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name SKMEL28
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
17
Experiment ID 259
MISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|FLOT1
EV Enriched markers
✘ EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name A375M
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
18
Experiment ID 260
MISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|FLOT1|CD81
EV Enriched markers
✘ EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name 1205Lu
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
19
Experiment ID 488
MISEV standards
✔
EM
EV Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
EV Enriched markers
✔
CANX
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
20
Experiment ID 224
MISEV standards
✔
EM|AFM
EV Biophysical techniques
✔
Alix|TSG101|CD63|CD81
EV Enriched markers
✔
GOLGA2
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25944692    
Organism Homo sapiens
Experiment description Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes
Authors Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S
Journal name Oncotarget
Publication year 2015
Sample Neuroblastoma cells
Sample name SH-SY5Y
Isolation/purification methods Differential centrifugation
Ultracentrifugation
OptiPrep density gradient
Flotation density 1.10 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
21
Experiment ID 231
MISEV standards
✘ EV Biophysical techniques
✔
Alix|CD63|CD9
EV Enriched markers
✘ EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G.
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Western blotting
Mass spectrometry
22
Experiment ID 232
MISEV standards
✘ EV Biophysical techniques
✘ EV Enriched markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G.
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
23
Experiment ID 233
MISEV standards
✘ EV Biophysical techniques
✘ EV Enriched markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G.
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
24
Experiment ID 274
MISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix|RAB5A|CD9|CD82|CD63|CD81
EV Enriched markers
✔
AIF
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25844599    
Organism Homo sapiens
Experiment description Molecular profiling of prostate cancer derived exosomes may reveal a predictive signature for response to docetaxel.
Authors Kharaziha P, Chioureas D, Rutishauser D, Baltatzis G, Lennartsson L, Fonseca P, Azimi A, Hultenby K, Zubarev R, Ullen A, Yachnin J, Nilsson S, Panaretakis T.
Journal name Oncotarget
Publication year 2015
Sample Prostate cancer cells
Sample name DU145 - Docetaxel resistant
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.18 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Flow cytometry
Western blotting
25
Experiment ID 834
MISEV standards
✔
EM
EV Biophysical techniques
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
EV Enriched markers
✔
CANX
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
26
Experiment ID 835
MISEV standards
✔
EM
EV Biophysical techniques
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
EV Enriched markers
✔
CANX
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
27
Experiment ID 217
MISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|CD81|CD9|CD63
EV Enriched markers
✘ EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O.
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
28
Experiment ID 196
MISEV standards
✔
EM
EV Biophysical techniques
✔
Alix|TSG101|HSP70|CD9
EV Enriched markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 22418980    
Organism Homo sapiens
Experiment description A multiplex quantitative proteomics strategy for protein biomarker studies in urinary exosomes.
Authors Raj DA, Fiume I, Capasso G, Pocsfalvi G.
Journal name Kidney Int
Publication year 2012
Sample Urine
Sample name Urine - Normal high density
Isolation/purification methods Differential centrifugation
Sucrose cushion
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
29
Experiment ID 197
MISEV standards
✔
EM
EV Biophysical techniques
✔
Alix|TSG101|HSP70|CD9
EV Enriched markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 22418980    
Organism Homo sapiens
Experiment description A multiplex quantitative proteomics strategy for protein biomarker studies in urinary exosomes.
Authors Raj DA, Fiume I, Capasso G, Pocsfalvi G.
Journal name Kidney Int
Publication year 2012
Sample Urine
Sample name Urine - Normal low density
Isolation/purification methods Differential centrifugation
Sucrose cushion
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for FYN
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 CD48 962
Invitro Homo sapiens
Affinity Capture-MS Homo sapiens
2 CSK 1445
Affinity Capture-Western Homo sapiens
3 DAG1 1605
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
4 CD2AP 23607
Invivo Homo sapiens
Invitro Homo sapiens
5 SOCS1  
Reconstituted Complex Homo sapiens
6 GRB10 2887
Biochemical Activity Homo sapiens
7 EVL 51466
Affinity Capture-MS Homo sapiens
8 CD247 919
Affinity Capture-MS Homo sapiens
9 EPHA8  
Invivo Homo sapiens
Invitro Homo sapiens
Affinity Capture-MS Homo sapiens
10 CD2 914
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
11 WAS 7454
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Invivo Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
12 PLCG2 5336
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
13 ARHGAP32  
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
14 PDGFRB 5159
Affinity Capture-MS Homo sapiens
15 ZAP70 7535
Phenotypic Enhancement Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
16 YES1 7525
Affinity Capture-Western Homo sapiens
17 SRC 6714
Protein-peptide Homo sapiens
18 KHDRBS1 10657
Invivo Homo sapiens
Invitro Homo sapiens
Reconstituted Complex Homo sapiens
19 SYK 6850
Invivo Homo sapiens
Invitro Homo sapiens
Affinity Capture-MS Homo sapiens
20 YT521  
Biochemical Activity Homo sapiens
21 TUBA3C 7278
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
22 CDK1 983
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
23 CD44 960
Invitro Homo sapiens
Invivo Homo sapiens
Affinity Capture-MS Homo sapiens
24 NMT1 4836
Biochemical Activity Homo sapiens
25 MCAM 4162
Affinity Capture-Western Homo sapiens
26 TRPV4 59341
Invitro Homo sapiens
Invivo Homo sapiens
27 FAS 355
Affinity Capture-MS Homo sapiens
Invivo Homo sapiens
28 MAG  
Invivo Homo sapiens
Invitro Homo sapiens
29 NEDD9 4739
Affinity Capture-Western Homo sapiens
30 PLAUR 5329
Invitro Homo sapiens
Invivo Homo sapiens
Affinity Capture-MS Homo sapiens
31 FASLG 356
Invitro Homo sapiens
32 VAV1 7409
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
33 SKAP1 8631
Reconstituted Complex Homo sapiens
34 NPHS1 4868
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
35 SKAP2 8935
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
36 CBL 867
Invivo Homo sapiens
Invitro Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
37 PAG1 55824
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
38 ADD2 119
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
39 MAPT  
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
40 CDH1 999
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
41 TUBA1B 10376
Affinity Capture-MS Homo sapiens
42 FYN 2534
Invitro Homo sapiens
Affinity Capture-MS Homo sapiens
Invitro Homo sapiens
Affinity Capture-MS Homo sapiens
43 TYK2 7297
Invivo Homo sapiens
Invitro Homo sapiens
Affinity Capture-MS Homo sapiens
44 PTK2B 2185
Affinity Capture-Western Homo sapiens
Invivo Homo sapiens
Invivo Homo sapiens
Invitro Homo sapiens
Invitro Homo sapiens
45 CNTN1 1272
Invivo Homo sapiens
46 UNC119 9094
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
47 PIK3R1 5295
Reconstituted Complex Homo sapiens
48 BTK 695
Reconstituted Complex Homo sapiens
49 GRIN2A  
Invivo Homo sapiens
Invitro Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
50 SH3BP2  
Two-hybrid Homo sapiens
51 PECAM1 5175
Affinity Capture-Western Homo sapiens
52 SH2D1A 4068
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
53 PTPN11 5781
Invitro Homo sapiens
Affinity Capture-MS Homo sapiens
54 SDC3  
Invitro Homo sapiens
Affinity Capture-MS Homo sapiens
55 Mta3  
Reconstituted Complex Mus musculus
56 DLG4  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
57 CTNNB1 1499
Biochemical Activity Homo sapiens
58 PTPRA 5786
Invivo Homo sapiens
Affinity Capture-MS Homo sapiens
59 CHRNA7  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
60 PTK2 5747
Invitro Homo sapiens
61 CD226 10666
Invivo Homo sapiens
62 TUBA4A 7277
Affinity Capture-MS Homo sapiens
63 PRKCQ 5588
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
64 IL7R  
Invivo Homo sapiens
Invitro Homo sapiens
65 BCL3  
Invivo Homo sapiens
66 GRIN2B  
Invitro Homo sapiens
67 RAF1 5894
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
68 SIT1 27240
Invivo Homo sapiens
69 TRPC6 7225
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
70 CD55 1604
Affinity Capture-MS Homo sapiens
71 EPHB3 2049
Reconstituted Complex Homo sapiens
72 CSF1R  
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
73 THY1 7070
Invivo Homo sapiens
Affinity Capture-MS Homo sapiens
74 ITK 3702
Invivo Homo sapiens
Invitro Homo sapiens
Reconstituted Complex Homo sapiens
75 CBLC  
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
76 SNCA 6622
Biochemical Activity Homo sapiens
77 TNK2 10188
Invivo Homo sapiens
Invitro Homo sapiens
Affinity Capture-MS Homo sapiens
78 FNBP4  
Invitro Homo sapiens
Affinity Capture-MS Homo sapiens
79 GAB3  
Reconstituted Complex Homo sapiens
80 FYB 2533
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
81 RICS  
Two-hybrid Homo sapiens
82 NTRK2 4915
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
83 MAP2 4133
Affinity Capture-Western Homo sapiens
84 EPHA4 2043
Affinity Capture-MS Homo sapiens
Invivo Homo sapiens
85 MS4A1 931
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
86 TRAT1 50852
Reconstituted Complex Homo sapiens
87 SOS1 6654
Invitro Homo sapiens
88 ADAM15 8751
Invivo Homo sapiens
Affinity Capture-MS Homo sapiens
89 SLAMF1 6504
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
90 HNRNPK 3190
Affinity Capture-MS Homo sapiens
91 CD36 948
Affinity Capture-Western Homo sapiens
Invivo Homo sapiens
92 Mta1  
Reconstituted Complex Mus musculus
Reconstituted Complex Mus musculus
93 PTPN5  
Invivo Homo sapiens
94 FCER2 2208
Affinity Capture-MS Homo sapiens
95 PXN 5829
Invivo Homo sapiens
96 PIK3R2 5296
Reconstituted Complex Homo sapiens
Biochemical Activity Homo sapiens
97 GNB2L1 10399
Affinity Capture-Western Homo sapiens
Invivo Homo sapiens
Invitro Homo sapiens
Affinity Capture-MS Homo sapiens
98 GP6 51206
Invitro Homo sapiens
99 BCAR1 9564
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
100 JAK2 3717
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
101 CTNND1 1500
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
102 CTLA4  
Invivo Homo sapiens
Invitro Homo sapiens
View the network image/svg+xml
 Pathways in which FYN is involved
PathwayEvidenceSource
Antigen activates B Cell Receptor (BCR) leading to generation of second messengers TAS Reactome
CD209 (DC-SIGN) signaling TAS Reactome
CD28 co-stimulation TAS Reactome
CD28 dependent PI3K/Akt signaling TAS Reactome
CD28 dependent Vav1 pathway TAS Reactome
Cell surface interactions at the vascular wall TAS Reactome
Constitutive Signaling by Aberrant PI3K in Cancer TAS Reactome
CRMPs in Sema3A signaling TAS Reactome
CTLA4 inhibitory signaling TAS Reactome
DAP12 signaling TAS Reactome
DCC mediated attractive signaling TAS Reactome
Dectin-2 family TAS Reactome
EPH-ephrin mediated repulsion of cells TAS Reactome
EPH-Ephrin signaling TAS Reactome
EPHA-mediated growth cone collapse TAS Reactome
EPHB-mediated forward signaling TAS Reactome
Ephrin signaling TAS Reactome
FCGR activation TAS Reactome
GPVI-mediated activation cascade TAS Reactome
NCAM signaling for neurite out-growth TAS Reactome
Nef and signal transduction TAS Reactome
Nephrin interactions TAS Reactome
Netrin mediated repulsion signals TAS Reactome
PECAM1 interactions TAS Reactome
PIP3 activates AKT signaling TAS Reactome
Platelet Adhesion to exposed collagen TAS Reactome
Regulation of KIT signaling TAS Reactome
Regulation of signaling by CBL TAS Reactome
Role of LAT2/NTAL/LAB on calcium mobilization TAS Reactome
Sema3A PAK dependent Axon repulsion TAS Reactome
SEMA3A-Plexin repulsion signaling by inhibiting Integrin adhesion TAS Reactome
Signaling by ERBB2 TAS Reactome
Signaling by SCF-KIT TAS Reactome
VEGFA-VEGFR2 Pathway TAS Reactome





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