Gene description for NPHS1
Gene name nephrosis 1, congenital, Finnish type (nephrin)
Gene symbol NPHS1
Other names/aliases CNF
NPHN
nephrin
Species Homo sapiens
 Database cross references - NPHS1
ExoCarta ExoCarta_4868
Entrez Gene 4868
HGNC 7908
MIM 602716
UniProt O60500  
 NPHS1 identified in exosomes derived from the following tissue/cell type
Urine 19056867    
 Gene ontology annotations for NPHS1
Molecular Function
    protein domain specific binding GO:0019904 IEA
    myosin binding GO:0017022 IPI
    spectrin binding GO:0030507 IEA
    alpha-actinin binding GO:0051393 IEA
    protein binding GO:0005515 IPI
Biological Process
    glomerular visceral epithelial cell development GO:0072015 IEP
    glomerular basement membrane development GO:0032836 IEP
    JNK cascade GO:0007254 IEA
    regulation of excretion GO:0044062 IEA
    positive regulation of actin filament polymerization GO:0030838 IEA
    cell adhesion GO:0007155 IEA
    myoblast fusion GO:0007520 IEA
    excretion GO:0007588 TAS
    skeletal muscle tissue development GO:0007519 IEA
Subcellular Localization
    cell projection GO:0042995 IEA
    extracellular exosome GO:0070062 IDA
    plasma membrane GO:0005886 TAS
    protein complex GO:0043234 IEA
    intracellular GO:0005622 IEA
    membrane raft GO:0045121 IEA
    integral component of plasma membrane GO:0005887 TAS
    slit diaphragm GO:0036057 ISS
 Experiment description of studies that identified NPHS1 in exosomes
1
Experiment ID 63
ISEV standards
EV Biophysical techniques
EV Cytosolic markers
AQP2
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19056867    
Organism Homo sapiens
Experiment description Large-scale proteomics and phosphoproteomics of urinary exosomes.
Authors Gonzales PA, Pisitkun T, Hoffert JD, Tchapyjnikov D, Star RA, Kleta R, Wang NS, Knepper MA
Journal name JASN
Publication year 2009
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
 Protein-protein interactions for NPHS1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 NPHS2 7827
Invivo Homo sapiens
Invivo Homo sapiens
Invitro Homo sapiens
Invitro Homo sapiens
2 YES1 7525
Affinity Capture-Western Homo sapiens
3 CD2AP 23607
Affinity Capture-Western Homo sapiens
Invivo Homo sapiens
Reconstituted Complex Homo sapiens
4 KIRREL 55243
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
5 TJP1 7082
Reconstituted Complex Homo sapiens
6 CDH3 1001
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
7 FYN 2534
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
8 NPHS1 4868
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
9 CTNND1 1500
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
10 CASK 8573
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
View the network image/svg+xml
 Pathways in which NPHS1 is involved
No pathways found





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