Gene description for Cdc42
Gene name cell division cycle 42
Gene symbol Cdc42
Other names/aliases AI747189
AU018915
Species Mus musculus
 Database cross references - Cdc42
ExoCarta ExoCarta_12540
Entrez Gene 12540
UniProt P60766  
 Cdc42 identified in exosomes derived from the following tissue/cell type
Fibroblasts 23260141    
Macrophages 23658846    
Macrophages 23658846    
Macrophages 23658846    
Mov neuroglial cells 15210972    
 Gene ontology annotations for Cdc42
Molecular Function
    protein kinase binding GO:0019901 IPI
    nucleotide binding GO:0000166 IEA
    GTP binding GO:0005525 ISO
    GTP-dependent protein binding GO:0030742 IPI
    mitogen-activated protein kinase kinase kinase binding GO:0031435 ISO
    GTPase activity GO:0003924 TAS
    protein binding GO:0005515 IPI
Biological Process
    establishment or maintenance of apical/basal cell polarity GO:0035088 IMP
    positive regulation of synapse structural plasticity GO:0051835 ISO
    regulation of protein stability GO:0031647 IMP
    positive regulation of DNA replication GO:0045740 ISO
    canonical Wnt signaling pathway GO:0060070 IMP
    positive regulation of neuron apoptotic process GO:0043525 ISO
    regulation of mitotic nuclear division GO:0007088 IMP
    epithelial-mesenchymal cell signaling GO:0060684 IMP
    neuron fate determination GO:0048664 IMP
    organelle transport along microtubule GO:0072384 ISO
    positive regulation of metalloenzyme activity GO:0048554 ISO
    regulation of protein catabolic process GO:0042176 IMP
    positive regulation of JNK cascade GO:0046330 ISO
    regulation of protein kinase activity GO:0045859 IMP
    epidermis morphogenesis GO:0048730 IMP
    nervous system development GO:0007399 IEA
    endosomal transport GO:0016197 TAS
    single organismal cell-cell adhesion GO:0016337 TAS
    keratinocyte development GO:0003334 IMP
    establishment or maintenance of cell polarity GO:0007163 TAS
    hair follicle morphogenesis GO:0031069 IMP
    heart contraction GO:0060047 IGI
    epithelial cell-cell adhesion GO:0090136 IMP
    positive regulation of peptidyl-serine phosphorylation GO:0033138 IMP
    regulation of protein heterodimerization activity GO:0043497 IDA
    actin filament organization GO:0007015 ISO
    positive regulation of hair follicle cell proliferation GO:0071338 IMP
    hair follicle placode formation GO:0060789 IMP
    regulation of protein metabolic process GO:0051246 IMP
    sprouting angiogenesis GO:0002040 ISO
    positive regulation of protein phosphorylation GO:0001934 IMP
    adherens junction organization GO:0034332 IMP
    positive regulation of gene expression GO:0010628 IMP
    keratinization GO:0031424 IMP
    cardiac conduction system development GO:0003161 IGI
    Golgi organization GO:0007030 ISO
    small GTPase mediated signal transduction GO:0007264 IEA
    cellular protein localization GO:0034613 IMP
    establishment of Golgi localization GO:0051683 ISO
    Rho protein signal transduction GO:0007266 TAS
    positive regulation of MAPK cascade GO:0043410 IDA
    filopodium assembly GO:0046847 IDA
    dendritic cell migration GO:0036336 IGI
    actin filament bundle assembly GO:0051017 IMP
    actin filament branching GO:0090135 ISO
    multicellular organism growth GO:0035264 IMP
    positive regulation of intracellular protein transport GO:0090316 ISO
    nucleus localization GO:0051647 IMP
    nuclear migration GO:0007097 IMP
    endocytosis GO:0006897 IMP
    cell differentiation GO:0030154 IEA
    positive regulation of phosphatidylinositol 3-kinase activity GO:0043552 IMP
    negative regulation of gene expression GO:0010629 IMP
Subcellular Localization
    myelin sheath GO:0043209 IDA
    membrane GO:0016020 IEA
    cell-cell junction GO:0005911 IDA
    cell GO:0005623 IMP
    cell periphery GO:0071944 IDA
    cell projection GO:0042995 IDA
    apical part of cell GO:0045177 IDA
    cytoplasm GO:0005737 IDA
    leading edge membrane GO:0031256 IDA
    Golgi membrane GO:0000139 ISO
    cytoskeleton GO:0005856 IEA
    cytosol GO:0005829 IDA
    storage vacuole GO:0000322 IDA
    plasma membrane GO:0005886 ISO
    intracellular GO:0005622 TAS
    secretory granule GO:0030141 ISO
 Experiment description of studies that identified Cdc42 in exosomes
1
Experiment ID 210
ISEV standards
EM
EV Biophysical techniques
EV Cytosolic markers
CD81|FLOT1
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23260141    
Organism Mus musculus
Experiment description Exosomes Mediate Stromal Mobilization of Autocrine Wnt-PCP Signaling in Breast Cancer Cell Migration.
Authors Luga V, Zhang L, Viloria-Petit AM, Ogunjimi AA, Inanlou MR, Chiu E, Buchanan M, Hosein AN, Basik M, Wrana JL.
Journal name Cell
Publication year 2012
Sample Fibroblasts
Sample name Normal-Fibroblasts (L cells)
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
2
Experiment ID 214
ISEV standards
EM
EV Biophysical techniques
EV Cytosolic markers
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors Hassani K, Olivier M.
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name Leishmania-infected-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 215
ISEV standards
EM
EV Biophysical techniques
EV Cytosolic markers
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors Hassani K, Olivier M.
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name LPS-treated-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 216
ISEV standards
EM
EV Biophysical techniques
EV Cytosolic markers
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors Hassani K, Olivier M.
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name Normal-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
5
Experiment ID 39
ISEV standards
IEM
EV Biophysical techniques
TSG101|HSC70
EV Cytosolic markers
FLOT1
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 15210972    
Organism Mus musculus
Experiment description Cells release prions in association with exosomes.
Authors Fevrier B, Vilette D, Archer F, Loew D, Faigle W, Vidal M, Laude H, Raposo G
Journal name PNAS
Publication year 2004
Sample Mov neuroglial cells
Sample name Mov neuroglial cell
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.14 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QSTAR]
Western blotting
Immunoelectron Microscopy
 Protein-protein interactions for Cdc42
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Arhgap32  
Biochemical Activity Mus musculus
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