Gene description for Rac1
Gene name RAS-related C3 botulinum substrate 1
Gene symbol Rac1
Other names/aliases AL023026
D5Ertd559e
Species Mus musculus
 Database cross references - Rac1
ExoCarta ExoCarta_19353
Vesiclepedia VP_19353
Entrez Gene 19353
UniProt P63001  
 Rac1 identified in exosomes derived from the following tissue/cell type
Colon cancer cells 37309723    
Embryonic fibroblasts 18494037    
Fibroblasts 23260141    
Mast cells 17486113    
Pancreatic cells 19351151    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Rac1
Molecular Function
    GTP binding GO:0005525 ISO
    protein binding GO:0005515 IPI
    GTP-dependent protein binding GO:0030742 IPI
    GTPase activity GO:0003924 ISO
    thioesterase binding GO:0031996 ISO
    Rab GTPase binding GO:0017137 ISO
    Rho GDP-dissociation inhibitor binding GO:0051022 ISO
    nucleotide binding GO:0000166 IEA
    histone deacetylase binding GO:0042826 ISO
    protein kinase binding GO:0019901 ISO
    enzyme binding GO:0019899 ISO
Biological Process
    positive regulation of focal adhesion assembly GO:0051894 ISO
    regulation of respiratory burst GO:0060263 ISO
    positive regulation of lamellipodium assembly GO:0010592 ISO
    engulfment of apoptotic cell GO:0043652 IDA
    axon guidance GO:0007411 IMP
    cell motility GO:0048870 ISO
    phagocytosis, engulfment GO:0006911 IMP
    embryonic olfactory bulb interneuron precursor migration GO:0021831 IMP
    anatomical structure arrangement GO:0048532 IMP
    positive regulation of neutrophil chemotaxis GO:0090023 ISO
    negative regulation of interleukin-23 production GO:0032707 ISO
    metabolic process GO:0008152 ISO
    chemotaxis GO:0006935 ISO
    positive regulation of cell-substrate adhesion GO:0010811 ISO
    synaptic transmission, GABAergic GO:0051932 IGI
    actin filament polymerization GO:0030041 IDA
    small GTPase mediated signal transduction GO:0007264 IDA
    endocytosis GO:0006897 IDA
    epithelial cell morphogenesis GO:0003382 IMP
    dendrite development GO:0016358 IDA
    mast cell chemotaxis GO:0002551 ISO
    localization within membrane GO:0051668 ISO
    protein localization to plasma membrane GO:0072659 IDA
    dopaminergic neuron differentiation GO:0071542 IGI
    cochlea morphogenesis GO:0090103 IMP
    dendrite morphogenesis GO:0048813 IGI
    cell migration GO:0016477 IDA
    cell proliferation GO:0008283 ISO
    lamellipodium assembly GO:0030032 TAS
    regulation of cell migration GO:0030334 ISO
    Wnt signaling pathway, planar cell polarity pathway GO:0060071 IGI
    cellular response to mechanical stimulus GO:0071260 ISO
    cerebral cortex GABAergic interneuron development GO:0021894 IGI
    actin filament organization GO:0007015 ISO
    cell adhesion GO:0007155 IDA
    regulation of fibroblast migration GO:0010762 IMP
    actin cytoskeleton organization GO:0030036 ISO
    positive regulation of protein phosphorylation GO:0001934 ISO
    ruffle organization GO:0031529 ISO
    substrate adhesion-dependent cell spreading GO:0034446 ISO
    cell-cell junction organization GO:0045216 IMP
    positive regulation of substrate adhesion-dependent cell spreading GO:1900026 ISO
    hyperosmotic response GO:0006972 IDA
    positive regulation of DNA replication GO:0045740 ISO
    cerebral cortex radially oriented cell migration GO:0021799 IMP
    regulation of neuron maturation GO:0014041 IGI
    cytoskeleton organization GO:0007010 IDA
    regulation of cell morphogenesis GO:0022604 IGI
    semaphorin-plexin signaling pathway GO:0071526 ISO
    bone resorption GO:0045453 ISO
    homeostasis of number of cells within a tissue GO:0048873 IGI
    ruffle assembly GO:0097178 IMP
    auditory receptor cell morphogenesis GO:0002093 IMP
    positive regulation of stress fiber assembly GO:0051496 ISO
    positive regulation of actin filament polymerization GO:0030838 IDA
    G-protein coupled receptor signaling pathway GO:0007186 IGI
    positive regulation of phosphatidylinositol 3-kinase activity GO:0043552 IMP
Subcellular Localization
    plasma membrane GO:0005886 IDA
    cytoplasmic vesicle GO:0031410 ISO
    extrinsic component of plasma membrane GO:0019897 IDA
    cell projection GO:0042995 IDA
    nucleus GO:0005634 IDA
    ruffle membrane GO:0032587 IDA
    extracellular exosome GO:0070062 ISO
    cytoplasmic ribonucleoprotein granule GO:0036464 ISO
    membrane GO:0016020 ISO
    lamellipodium GO:0030027 ISO
    focal adhesion GO:0005925 ISO
    cytoplasmic membrane-bounded vesicle GO:0016023 IDA
    actin filament GO:0005884 ISO
    phagocytic cup GO:0001891 IDA
    Golgi membrane GO:0000139 ISO
    trans-Golgi network GO:0005802 ISO
    cytoplasm GO:0005737 ISO
    intracellular GO:0005622 IEA
    cytosol GO:0005829 ISO
    early endosome membrane GO:0031901 IDA
 Experiment description of studies that identified Rac1 in exosomes
1
Experiment ID 907
MISEV standards
✔
EM
EV Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
EV Enriched markers
✔
HSPA5
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S.
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 73
MISEV standards
✔
EM
EV Biophysical techniques
✔
HSP70|HSP90
EV Enriched markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 18494037    
Organism Mus musculus
Experiment description Difference gel electrophoresis analysis of Ras-transformed fibroblast cell-derived exosomes.
Authors Ji H, Erfani N, Tauro BJ, Kapp EA, Zhu HJ, Moritz RL, Lim JW, Simpson RJ
Journal name ELEC
Publication year 2008
Sample Embryonic fibroblasts
Sample name NIH3T3
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LCQ DECA]
Western blotting
3
Experiment ID 210
MISEV standards
✔
EM
EV Biophysical techniques
✔
CD81|FLOT1
EV Enriched markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23260141    
Organism Mus musculus
Experiment description Exosomes Mediate Stromal Mobilization of Autocrine Wnt-PCP Signaling in Breast Cancer Cell Migration.
Authors Luga V, Zhang L, Viloria-Petit AM, Ogunjimi AA, Inanlou MR, Chiu E, Buchanan M, Hosein AN, Basik M, Wrana JL.
Journal name Cell
Publication year 2012
Sample Fibroblasts
Sample name Normal-Fibroblasts (L cells)
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 15
MISEV standards
✔
EM
EV Biophysical techniques
✔
CD63
EV Enriched markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
5
Experiment ID 188
MISEV standards
✔
EM
EV Biophysical techniques
✔
GAPDH|UCHL1|HSP90
EV Enriched markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19351151    
Organism Mus musculus
Experiment description Characterization of vesicles secreted from insulinoma NIT-1 cells.
Authors Lee HS, Jeong J, Lee KJ.
Journal name J Proteome Res
Publication year 2009
Sample Pancreatic cells
Sample name Pancreatic beta cell (NIT-1)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
6
Experiment ID 908
MISEV standards
✔
EM
EV Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
EV Enriched markers
✔
HSPA5
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S.
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Rac1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 SMURF2 64750
Affinity Capture-Luminescence Homo sapiens
2 N/A  
Biochemical Activity Mus musculus
View the network image/svg+xml
 Pathways in which Rac1 is involved
PathwayEvidenceSource
Activation of Rac IEA Reactome
CD28 dependent Vav1 pathway IEA Reactome
DAP12 signaling IEA Reactome
DCC mediated attractive signaling IEA Reactome
DSCAM interactions IEA Reactome
EPH-ephrin mediated repulsion of cells IEA Reactome
EPHB-mediated forward signaling IEA Reactome
Ephrin signaling IEA Reactome
Factors involved in megakaryocyte development and platelet production IEA Reactome
FCERI mediated MAPK activation IEA Reactome
G alpha (12/13) signalling events IEA Reactome
GPVI-mediated activation cascade IEA Reactome
Inactivation of Cdc42 and Rac IEA Reactome
NRAGE signals death through JNK IEA Reactome
PCP/CE pathway IEA Reactome
Regulation of actin dynamics for phagocytic cup formation IEA Reactome
Rho GTPase cycle IEA Reactome
RHO GTPases activate CIT IEA Reactome
RHO GTPases Activate Formins IEA Reactome
RHO GTPases activate IQGAPs IEA Reactome
RHO GTPases activate KTN1 IEA Reactome
RHO GTPases Activate NADPH Oxidases IEA Reactome
RHO GTPases activate PAKs IEA Reactome
RHO GTPases Activate WASPs and WAVEs IEA Reactome
Sema3A PAK dependent Axon repulsion IEA Reactome
SEMA3A-Plexin repulsion signaling by inhibiting Integrin adhesion IEA Reactome
Sema4D induced cell migration and growth-cone collapse IEA Reactome
Sema4D mediated inhibition of cell attachment and migration IEA Reactome
Signal transduction by L1 IEA Reactome
Signaling by SCF-KIT IEA Reactome
Translocation of GLUT4 to the plasma membrane IEA Reactome
VEGFA-VEGFR2 Pathway IEA Reactome
VEGFR2 mediated vascular permeability IEA Reactome





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