Gene description for Cyfip1
Gene name cytoplasmic FMR1 interacting protein 1
Gene symbol Cyfip1
Other names/aliases E030028J09Rik
P140SRA-1
P140sra1
Shyc
Sra-1
Sra1
l(7)1Rl
l71Rl
l7Rl1
mKIAA0068
pl-1
Species Mus musculus
 Database cross references - Cyfip1
ExoCarta ExoCarta_20430
Vesiclepedia VP_20430
Entrez Gene 20430
UniProt Q7TMB8  
 Cyfip1 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
Macrophages 23658846    
Macrophages 23658846    
Macrophages 23658846    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Cyfip1
Molecular Function
    RNA 7-methylguanosine cap binding GO:0000340 IBA
    RNA 7-methylguanosine cap binding GO:0000340 IEA
    RNA 7-methylguanosine cap binding GO:0000340 ISO
    protein binding GO:0005515 IPI
    profilin binding GO:0005522 TAS
    translation repressor activity GO:0030371 IDA
    small GTPase binding GO:0031267 ISO
    small GTPase binding GO:0031267 ISS
    actin filament binding GO:0051015 ISS
Biological Process
    cell morphogenesis GO:0000902 IBA
    regulation of translation GO:0006417 IBA
    axon guidance GO:0007411 IBA
    regulation of cell shape GO:0008360 IEA
    positive regulation of lamellipodium assembly GO:0010592 IEA
    positive regulation of lamellipodium assembly GO:0010592 ISO
    Rac protein signal transduction GO:0016601 IEA
    Rac protein signal transduction GO:0016601 ISO
    negative regulation of translation GO:0017148 IEA
    lamellipodium assembly GO:0030032 IBA
    lamellipodium assembly GO:0030032 IDA
    regulation of actin filament polymerization GO:0030833 IEA
    neuron projection development GO:0031175 IMP
    ruffle organization GO:0031529 ISS
    regulation of myelination GO:0031641 IEA
    regulation of myelination GO:0031641 ISO
    cellular response to insulin stimulus GO:0032869 IEA
    cellular response to insulin stimulus GO:0032869 ISO
    positive regulation of axon extension GO:0045773 IEA
    positive regulation of axon extension GO:0045773 ISO
    axon extension GO:0048675 ISO
    axon extension GO:0048675 ISS
    positive regulation of axonogenesis GO:0050772 ISO
    cognition GO:0050890 IEA
    cognition GO:0050890 ISO
    positive regulation of neurotrophin TRK receptor signaling pathway GO:0051388 IMP
    response to electrical stimulus GO:0051602 IEA
    response to electrical stimulus GO:0051602 ISO
    dendrite extension GO:0097484 IMP
    modification of synaptic structure GO:0099563 IBA
    regulation of translation at postsynapse, modulating synaptic transmission GO:0099578 EXP
    regulation of translation at postsynapse, modulating synaptic transmission GO:0099578 IDA
    regulation of translation at postsynapse, modulating synaptic transmission GO:0099578 IEP
    regulation of translation at postsynapse, modulating synaptic transmission GO:0099578 IMP
    positive regulation of dendrite development GO:1900006 IEA
    positive regulation of dendrite development GO:1900006 ISO
    positive regulation of ruffle assembly GO:1900029 IEA
    positive regulation of ruffle assembly GO:1900029 ISO
    negative regulation of synaptic vesicle recycling GO:1903422 IEA
    negative regulation of synaptic vesicle recycling GO:1903422 ISO
    regulation of modification of postsynaptic actin cytoskeleton GO:1905274 IDA
    regulation of modification of postsynaptic actin cytoskeleton GO:1905274 IMP
    positive regulation of Arp2/3 complex-mediated actin nucleation GO:2000601 IEA
    positive regulation of Arp2/3 complex-mediated actin nucleation GO:2000601 ISO
Subcellular Localization
    ruffle GO:0001726 ISS
    lamellipodium GO:0030027 IDA
    SCAR complex GO:0031209 IBA
    SCAR complex GO:0031209 IEA
    SCAR complex GO:0031209 ISO
    cell leading edge GO:0031252 IDA
    filopodium tip GO:0032433 IEA
    filopodium tip GO:0032433 ISO
    neuron projection GO:0043005 IBA
    neuron projection GO:0043005 IDA
    neuronal cell body GO:0043025 IEA
    neuronal cell body GO:0043025 ISO
    terminal bouton GO:0043195 IEA
    terminal bouton GO:0043195 ISO
    dendritic spine GO:0043197 IEA
    dendritic spine GO:0043197 ISO
    dendritic growth cone GO:0044294 IEA
    dendritic growth cone GO:0044294 ISO
    axonal growth cone GO:0044295 IEA
    axonal growth cone GO:0044295 ISO
    synapse GO:0045202 IBA
    perinuclear region of cytoplasm GO:0048471 IDA
    excitatory synapse GO:0060076 IEA
    excitatory synapse GO:0060076 ISO
    central region of growth cone GO:0090724 IEA
    central region of growth cone GO:0090724 ISO
    peripheral region of growth cone GO:0090725 IEA
    peripheral region of growth cone GO:0090725 ISO
    postsynapse GO:0098794 IDA
 Experiment description of studies that identified Cyfip1 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 214
MISEV standards
✔
EM
Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors "Hassani K, Olivier M."
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name Leishmania-infected-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 215
MISEV standards
✔
EM
Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors "Hassani K, Olivier M."
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name LPS-treated-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 216
MISEV standards
✔
EM
Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors "Hassani K, Olivier M."
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name Normal-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
5
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Cyfip1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Grm3  
Affinity Capture-MS Mus musculus
2 Rhoc 11853
Affinity Capture-MS Mus musculus
3 Tmem240  
Affinity Capture-MS Mus musculus
4 Matr3 17184
Affinity Capture-MS Mus musculus
5 Prkcg  
Affinity Capture-MS Mus musculus
6 Ntrk2  
Affinity Capture-MS Mus musculus
7 Dctn1 13191
Affinity Capture-MS Mus musculus
8 Mfn2  
Affinity Capture-MS Mus musculus
9 Pik3r1  
Affinity Capture-Western Mus musculus
10 Rpl27 19942
Affinity Capture-MS Mus musculus
11 Lrrc24  
Affinity Capture-MS Mus musculus
12 Cul3  
Affinity Capture-MS Mus musculus
13 Actb 11461
Affinity Capture-MS Mus musculus
14 Snap91  
Affinity Capture-MS Mus musculus
15 Sptbn1 20742
Affinity Capture-MS Mus musculus
16 Impdh2 23918
Affinity Capture-MS Mus musculus
17 Tmem173  
Proximity Label-MS Mus musculus
18 Ptpn9  
Affinity Capture-MS Mus musculus
19 Dync1h1 13424
Affinity Capture-MS Mus musculus
20 Abi1 11308
Affinity Capture-MS Mus musculus
21 Prepl  
Affinity Capture-MS Mus musculus
22 Rheb  
Affinity Capture-MS Mus musculus
23 Iqsec1  
Affinity Capture-MS Mus musculus
24 Trappc3  
Affinity Capture-MS Mus musculus
25 Ap1s1  
Affinity Capture-MS Mus musculus
26 Trpc4  
Affinity Capture-MS Mus musculus
27 Golga2  
Affinity Capture-MS Mus musculus
28 Apbb1ip  
Co-fractionation Mus musculus
29 Sptan1 20740
Affinity Capture-MS Mus musculus
30 Gsk3b  
Affinity Capture-MS Mus musculus
31 Capza2 12343
Affinity Capture-MS Mus musculus
32 Erc2 238988
Affinity Capture-MS Mus musculus
33 Kif2a 16563
Affinity Capture-MS Mus musculus
34 Tnik  
Affinity Capture-MS Mus musculus
35 Nefh  
Affinity Capture-MS Mus musculus
36 Tpd52l2  
Affinity Capture-MS Mus musculus
37 Dnm1l 74006
Affinity Capture-MS Mus musculus
38 Nefm 18040
Affinity Capture-MS Mus musculus
39 Dbn1  
Affinity Capture-MS Mus musculus
40 Cntnap1  
Affinity Capture-MS Mus musculus
41 Ap2b1 71770
Affinity Capture-MS Mus musculus
42 Eif4e  
Affinity Capture-Western Mus musculus
43 Dlg4  
Affinity Capture-MS Mus musculus
Affinity Capture-MS Mus musculus
Affinity Capture-MS Mus musculus
44 Dnajc6  
Affinity Capture-MS Mus musculus
45 Ube2o 217342
Affinity Capture-MS Mus musculus
46 Dnm2 13430
Affinity Capture-MS Mus musculus
47 Wasf2 242687
Co-fractionation Mus musculus
48 Epb4.1l3 13823
Affinity Capture-MS Mus musculus
49 Agap2  
Affinity Capture-MS Mus musculus
Affinity Capture-MS Mus musculus
50 Sgip1  
Affinity Capture-MS Mus musculus
51 Dclk1  
Affinity Capture-MS Mus musculus
52 Rtn1  
Affinity Capture-MS Mus musculus
53 Sptbn2  
Affinity Capture-MS Mus musculus
54 Arhgef2  
Affinity Capture-MS Mus musculus
55 Ncdn  
Affinity Capture-MS Mus musculus
56 Trim3  
Affinity Capture-MS Mus musculus
57 Rac1 19353
Affinity Capture-MS Mus musculus
58 Npepps 19155
Affinity Capture-MS Mus musculus
59 Ank2  
Affinity Capture-MS Mus musculus
60 Ipo4 75751
Affinity Capture-MS Mus musculus
61 Dstn 56431
Affinity Capture-MS Mus musculus
62 Dnm3 103967
Affinity Capture-MS Mus musculus
63 P2rx7 18439
Affinity Capture-MS Mus musculus
64 Glul 14645
Affinity Capture-MS Mus musculus
65 Opa1  
Affinity Capture-MS Mus musculus
66 Pfn2  
Affinity Capture-MS Mus musculus
67 Myo5a 17918
Affinity Capture-MS Mus musculus
68 Cct3 12462
Affinity Capture-MS Mus musculus
69 Pde4a  
Affinity Capture-MS Mus musculus
70 Dnm1 13429
Affinity Capture-MS Mus musculus
71 Camkv  
Affinity Capture-MS Mus musculus
72 Arpc4 68089
Affinity Capture-MS Mus musculus
73 Ncan  
Affinity Capture-MS Mus musculus
74 Numbl  
Affinity Capture-MS Mus musculus
75 Slc4a8  
Affinity Capture-MS Mus musculus
76 Nckap1 50884
Affinity Capture-MS Mus musculus
Affinity Capture-MS Mus musculus
77 Nyap2  
Affinity Capture-MS Mus musculus
Affinity Capture-MS Mus musculus
78 Crmp1 12933
Affinity Capture-MS Mus musculus
79 Grin2a  
Affinity Capture-MS Mus musculus
80 Nefl  
Affinity Capture-MS Mus musculus
81 Prkce  
Affinity Capture-MS Mus musculus
82 Sbf1  
Affinity Capture-MS Mus musculus
83 Kcnb1  
Affinity Capture-MS Mus musculus
84 Eed  
Affinity Capture-MS Mus musculus
85 Pi4ka 224020
Affinity Capture-MS Mus musculus
86 Ylpm1  
Affinity Capture-MS Mus musculus
87 Camk2b 12323
Affinity Capture-MS Mus musculus
88 Ppp1cc 19047
Affinity Capture-MS Mus musculus
89 Prox1  
Affinity Capture-MS Mus musculus
90 Wasf1  
Affinity Capture-Western Mus musculus
Affinity Capture-MS Mus musculus
Co-fractionation Mus musculus
91 Camk2a  
Affinity Capture-MS Mus musculus
92 Ap1b1 11764
Affinity Capture-MS Mus musculus
93 Cyfip2 76884
Affinity Capture-MS Mus musculus
Affinity Capture-MS Mus musculus
94 Prkcb  
Affinity Capture-MS Mus musculus
95 Rps6kb1  
Affinity Capture-MS Mus musculus
96 Nsf 18195
Affinity Capture-MS Mus musculus
97 Dpysl2 12934
Affinity Capture-MS Mus musculus
98 Dlgap1  
Affinity Capture-MS Mus musculus
Affinity Capture-MS Mus musculus
99 Phactr1  
Affinity Capture-MS Mus musculus
100 Syngap1  
Affinity Capture-MS Mus musculus
Affinity Capture-MS Mus musculus
101 Kctd13  
Affinity Capture-MS Mus musculus
102 Rapgef2  
Affinity Capture-MS Mus musculus
103 Macf1  
Affinity Capture-MS Mus musculus
104 Shank3  
Affinity Capture-MS Mus musculus
Affinity Capture-MS Mus musculus
Affinity Capture-MS Mus musculus
105 Chaf1a  
Affinity Capture-MS Mus musculus
106 Fermt3 108101
Affinity Capture-MS Mus musculus
107 Baiap2 108100
Affinity Capture-MS Mus musculus
108 Synpo  
Affinity Capture-MS Mus musculus
109 Shank1 243961
Affinity Capture-MS Mus musculus
110 Pdcd6ip 18571
Affinity Capture-MS Mus musculus
111 Aak1 269774
Affinity Capture-MS Mus musculus
112 Uba6 231380
Affinity Capture-MS Mus musculus
113 Ankrd17  
Affinity Capture-MS Mus musculus
114 Eef1a2 13628
Affinity Capture-MS Mus musculus
115 Tsc1  
Affinity Capture-MS Mus musculus
116 Ap2a1 11771
Affinity Capture-MS Mus musculus
117 Lnx1  
Affinity Capture-MS Mus musculus
118 Farp1 223254
Affinity Capture-MS Mus musculus
119 Dpysl4  
Affinity Capture-MS Mus musculus
120 Etf1 225363
Affinity Capture-MS Mus musculus
121 Dlg2 23859
Affinity Capture-MS Mus musculus
122 Epb4.1l1 13821
Affinity Capture-MS Mus musculus
123 Pak1  
Affinity Capture-MS Mus musculus
124 Picalm  
Affinity Capture-MS Mus musculus
125 Arf3 11842
Affinity Capture-MS Mus musculus
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