Gene description for Dnm3
Gene name dynamin 3
Gene symbol Dnm3
Other names/aliases 9630020E24Rik
AW061159
B230343F03Rik
C530045C17
mKIAA0820
Species Mus musculus
 Database cross references - Dnm3
ExoCarta ExoCarta_103967
Vesiclepedia VP_103967
Entrez Gene 103967
UniProt Q8BZ98  
 Dnm3 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Dnm3
Molecular Function
    GTPase activity GO:0003924 IBA
    GTPase activity GO:0003924 ISO
    protein binding GO:0005515 IPI
    GTP binding GO:0005525 IEA
    microtubule binding GO:0008017 IBA
    type 1 metabotropic glutamate receptor binding GO:0031798 ISO
    type 5 metabotropic glutamate receptor binding GO:0031802 ISO
    identical protein binding GO:0042802 ISO
    nitric-oxide synthase binding GO:0050998 ISO
    structural constituent of postsynapse GO:0099186 ISO
    protein serine/threonine kinase binding GO:0120283 ISO
    membrane scission GTPase motor activity GO:1990606 IEA
Biological Process
    endocytosis GO:0006897 ISO
    synapse assembly GO:0007416 ISO
    synaptic vesicle budding from presynaptic endocytic zone membrane GO:0016185 IBA
    receptor internalization GO:0031623 IBA
    filopodium assembly GO:0046847 ISO
    synaptic vesicle endocytosis GO:0048488 IDA
    synaptic vesicle endocytosis GO:0048488 IMP
    positive regulation of filopodium assembly GO:0051491 ISO
    regulation of dendritic spine morphogenesis GO:0061001 ISO
    negative regulation of dendritic spine morphogenesis GO:0061002 ISO
    postsynaptic neurotransmitter receptor internalization GO:0098884 ISO
    positive regulation of synaptic vesicle recycling GO:1903423 ISO
Subcellular Localization
    photoreceptor inner segment GO:0001917 IDA
    cytoplasm GO:0005737 IBA
    mitochondrion GO:0005739 HDA
    microtubule GO:0005874 IBA
    microtubule GO:0005874 IEA
    plasma membrane GO:0005886 IBA
    postsynaptic density GO:0014069 ISO
    axon GO:0030424 ISO
    synaptic cleft GO:0043083 ISO
    dendritic spine GO:0043197 ISO
    dendritic spine head GO:0044327 ISO
    synapse GO:0045202 IBA
    perinuclear region of cytoplasm GO:0048471 ISO
    apical tubulobulbar complex GO:0061828 ISO
    basal tubulobulbar complex GO:0061829 ISO
    presynapse GO:0098793 IDA
    postsynaptic endocytic zone membrane GO:0098844 ISO
    postsynaptic endocytic zone membrane GO:0098844 ISO
    glutamatergic synapse GO:0098978 ISO
 Experiment description of studies that identified Dnm3 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Dnm3
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Cyfip1 20430
Affinity Capture-MS Mus musculus
2 Gab1  
Co-fractionation Mus musculus
3 Krt36  
Co-fractionation Mus musculus
View the network image/svg+xml



Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here