Gene description for Impdh2
Gene name inosine 5'-phosphate dehydrogenase 2
Gene symbol Impdh2
Other names/aliases IMPD
Species Mus musculus
 Database cross references - Impdh2
ExoCarta ExoCarta_23918
Entrez Gene 23918
UniProt P24547  
 Impdh2 identified in exosomes derived from the following tissue/cell type
Macrophages 23658846    
Macrophages 23658846    
Macrophages 23658846    
 Gene ontology annotations for Impdh2
Molecular Function
    DNA binding GO:0003677 IEA
    catalytic activity GO:0003824 IEA
    RNA binding GO:0003723 IEA
    IMP dehydrogenase activity GO:0003938 ISO
    oxidoreductase activity GO:0016491 IEA
    metal ion binding GO:0046872 IEA
    nucleotide binding GO:0000166 ISO
Biological Process
    purine nucleotide biosynthetic process GO:0006164 ISO
    GMP biosynthetic process GO:0006177 IEA
    lymphocyte proliferation GO:0046651 IMP
    protein homotetramerization GO:0051289 ISO
    cellular response to interleukin-4 GO:0071353 IDA
    oxidation-reduction process GO:0055114 ISO
Subcellular Localization
    cytoplasm GO:0005737 ISO
    membrane GO:0016020 ISO
    nucleus GO:0005634 ISO
    peroxisomal membrane GO:0005778 ISO
    extracellular exosome GO:0070062 ISO
 Experiment description of studies that identified Impdh2 in exosomes
1
Experiment ID 214
ISEV standards
✔
EM
EV Biophysical techniques
✘ EV Cytosolic markers
✘ EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors Hassani K, Olivier M.
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name Leishmania-infected-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
2
Experiment ID 215
ISEV standards
✔
EM
EV Biophysical techniques
✘ EV Cytosolic markers
✘ EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors Hassani K, Olivier M.
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name LPS-treated-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 216
ISEV standards
✔
EM
EV Biophysical techniques
✘ EV Cytosolic markers
✘ EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors Hassani K, Olivier M.
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name Normal-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for Impdh2
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Impdh2 is involved
PathwayEvidenceSource
Purine ribonucleoside monophosphate biosynthesis IEA Reactome





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