Gene description for Npepps
Gene name aminopeptidase puromycin sensitive
Gene symbol Npepps
Other names/aliases AAP-S
MP100
Psa
R74825
goku
Species Mus musculus
 Database cross references - Npepps
ExoCarta ExoCarta_19155
Vesiclepedia VP_19155
Entrez Gene 19155
UniProt Q11011  
 Npepps identified in exosomes derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Npepps
Molecular Function
    hydrolase activity GO:0016787 IEA
    metallopeptidase activity GO:0008237 IEA
    zinc ion binding GO:0008270 IEA
    metal ion binding GO:0046872 IEA
    aminopeptidase activity GO:0004177 ISO
    peptidase activity GO:0008233 IEA
Biological Process
    proteolysis GO:0006508 ISO
    positive regulation of protein targeting to mitochondrion GO:1903955 ISO
    cellular response to hypoxia GO:0071456 ISO
Subcellular Localization
    extracellular exosome GO:0070062 ISO
    nucleus GO:0005634 IEA
    cytoplasm GO:0005737 IEA
 Experiment description of studies that identified Npepps in exosomes
1
Experiment ID 907
MISEV standards
✔
EM
EV Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
EV Enriched markers
✔
HSPA5
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S.
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
EV Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
EV Enriched markers
✔
HSPA5
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S.
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Npepps
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Npepps is involved
PathwayEvidenceSource
Antigen processing: Ubiquitination & Proteasome degradation IEA Reactome





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