Gene description for Pak2
Gene name p21 protein (Cdc42/Rac)-activated kinase 2
Gene symbol Pak2
Other names/aliases 5330420P17Rik
A130002K10Rik
AI836325
D16Ertd269e
PAK-2
mKIAA4182
Species Mus musculus
 Database cross references - Pak2
ExoCarta ExoCarta_224105
Vesiclepedia VP_224105
Entrez Gene 224105
UniProt Q8CIN4  
 Pak2 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Pak2
Molecular Function
    protein kinase activity GO:0004672 IDA
    protein serine/threonine kinase activity GO:0004674 IBA
    protein serine/threonine kinase activity GO:0004674 IDA
    protein serine/threonine kinase activity GO:0004674 ISO
    protein serine/threonine kinase activity GO:0004674 ISS
    3-phosphoinositide-dependent protein kinase activity GO:0004676 IEA
    DNA-dependent protein kinase activity GO:0004677 IEA
    AMP-activated protein kinase activity GO:0004679 IEA
    eukaryotic translation initiation factor 2alpha kinase activity GO:0004694 IEA
    ribosomal protein S6 kinase activity GO:0004711 IEA
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IEA
    protein kinase binding GO:0019901 IEA
    protein kinase binding GO:0019901 ISO
    protein tyrosine kinase activator activity GO:0030296 IEA
    protein tyrosine kinase activator activity GO:0030296 ISO
    small GTPase binding GO:0031267 IEA
    small GTPase binding GO:0031267 ISO
    histone H3S10 kinase activity GO:0035175 IEA
    histone H3T11 kinase activity GO:0035402 IEA
    histone H3T6 kinase activity GO:0035403 IEA
    histone H2AXS139 kinase activity GO:0035979 IEA
    identical protein binding GO:0042802 IEA
    identical protein binding GO:0042802 ISO
    histone H3S28 kinase activity GO:0044022 IEA
    histone H4S1 kinase activity GO:0044023 IEA
    histone H2AS1 kinase activity GO:0044024 IEA
    histone H2BS14 kinase activity GO:0044025 IEA
    histone H3T3 kinase activity GO:0072354 IEA
    histone H2AS121 kinase activity GO:0072371 IEA
    Rho-dependent protein serine/threonine kinase activity GO:0072518 IEA
    protein serine kinase activity GO:0106310 IEA
    histone H2BS36 kinase activity GO:0140823 IEA
    histone H3S57 kinase activity GO:0140855 IEA
    histone H3T45 kinase activity GO:0140857 IEA
    histone H2AT120 kinase activity GO:1990244 IEA
Biological Process
    cardiac muscle hypertrophy GO:0003300 IEA
    chromatin remodeling GO:0006338 IEA
    protein phosphorylation GO:0006468 ISO
    apoptotic process GO:0006915 IEA
    cellular response to starvation GO:0009267 IBA
    phosphorylation GO:0016310 ISO
    cell migration GO:0016477 IBA
    peptidyl-serine phosphorylation GO:0018105 ISO
    adherens junction assembly GO:0034333 IEA
    adherens junction assembly GO:0034333 ISO
    intracellular signal transduction GO:0035556 IBA
    negative regulation of apoptotic process GO:0043066 IEA
    negative regulation of apoptotic process GO:0043066 ISO
    regulation of MAPK cascade GO:0043408 IBA
    protein autophosphorylation GO:0046777 ISO
    positive regulation of peptidyl-tyrosine phosphorylation GO:0050731 IEA
    positive regulation of peptidyl-tyrosine phosphorylation GO:0050731 ISO
    regulation of axonogenesis GO:0050770 IBA
    regulation of cytoskeleton organization GO:0051493 ISO
    negative regulation of stress fiber assembly GO:0051497 IEA
    negative regulation of stress fiber assembly GO:0051497 ISO
    dendritic spine development GO:0060996 IGI
    bicellular tight junction assembly GO:0070830 IEA
    bicellular tight junction assembly GO:0070830 ISO
    cellular response to organic cyclic compound GO:0071407 IDA
    cellular response to transforming growth factor beta stimulus GO:0071560 IEA
    protein localization to cell-cell junction GO:0150105 IEA
    protein localization to cell-cell junction GO:0150105 ISO
    negative regulation of cysteine-type endopeptidase activity GO:2000117 ISO
    positive regulation of extrinsic apoptotic signaling pathway GO:2001238 IEA
    positive regulation of extrinsic apoptotic signaling pathway GO:2001238 ISO
Subcellular Localization
    nucleus GO:0005634 IEA
    nucleus GO:0005634 ISO
    cytoplasm GO:0005737 IBA
    cytoplasm GO:0005737 ISO
    cytoplasm GO:0005737 ISO
    cytosol GO:0005829 IEA
    cytosol GO:0005829 ISO
    cell-cell junction GO:0005911 IEA
    cell-cell junction GO:0005911 ISO
    postsynaptic density GO:0014069 IDA
    postsynaptic density GO:0014069 IEP
    membrane GO:0016020 IEA
    secretory granule GO:0030141 IEA
    secretory granule GO:0030141 ISO
    perinuclear region of cytoplasm GO:0048471 IEA
    glutamatergic synapse GO:0098978 IDA
    glutamatergic synapse GO:0098978 IEP
 Experiment description of studies that identified Pak2 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Pak2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Fxr1 14359
Affinity Capture-MS Mus musculus
2 Tmem173  
Proximity Label-MS Mus musculus
3 Scly  
Co-fractionation Mus musculus
4 Eed  
Affinity Capture-MS Mus musculus
5 Isoc1 66307
Co-fractionation Mus musculus
6 Npepps 19155
Co-fractionation Mus musculus
7 Tcf3  
Affinity Capture-MS Mus musculus
View the network image/svg+xml
 Pathways in which Pak2 is involved
PathwayEvidenceSource
Adaptive Immune System IEA Reactome
Axon guidance IEA Reactome
C-type lectin receptors (CLRs) IEA Reactome
CD209 (DC-SIGN) signaling IEA Reactome
CD28 co-stimulation IEA Reactome
CD28 dependent Vav1 pathway IEA Reactome
Costimulation by the CD28 family IEA Reactome
Developmental Biology IEA Reactome
EPH-Ephrin signaling IEA Reactome
Ephrin signaling IEA Reactome
Fc epsilon receptor (FCERI) signaling IEA Reactome
FCERI mediated MAPK activation IEA Reactome
Generation of second messenger molecules IEA Reactome
Immune System IEA Reactome
Innate Immune System IEA Reactome
MAPK family signaling cascades IEA Reactome
MAPK6/MAPK4 signaling IEA Reactome
Muscle contraction IEA Reactome
Nervous system development IEA Reactome
RAC1 GTPase cycle IEA Reactome
RAC2 GTPase cycle IEA Reactome
RAC3 GTPase cycle IEA Reactome
RHO GTPase cycle IEA Reactome
RHO GTPase Effectors IEA Reactome
RHO GTPases activate PAKs IEA Reactome
RHOG GTPase cycle IEA Reactome
RHOH GTPase cycle IEA Reactome
RHOQ GTPase cycle IEA Reactome
RHOU GTPase cycle IEA Reactome
RHOV GTPase cycle IEA Reactome
Sema3A PAK dependent Axon repulsion IEA Reactome
Semaphorin interactions IEA Reactome
Signal Transduction IEA Reactome
Signaling by Receptor Tyrosine Kinases IEA Reactome
Signaling by Rho GTPases IEA Reactome
Signaling by Rho GTPases, Miro GTPases and RHOBTB3 IEA Reactome
Signaling by VEGF IEA Reactome
Smooth Muscle Contraction IEA Reactome
TCR signaling IEA Reactome
VEGFA-VEGFR2 Pathway IEA Reactome
VEGFR2 mediated vascular permeability IEA Reactome





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