Gene description for Wasf2
Gene name WAS protein family, member 2
Gene symbol Wasf2
Other names/aliases AW742646
D4Ertd13e
WAVE2
Species Mus musculus
 Database cross references - Wasf2
ExoCarta ExoCarta_242687
Entrez Gene 242687
UniProt Q8BH43  
 Wasf2 identified in exosomes derived from the following tissue/cell type
Macrophages 23658846    
Macrophages 23658846    
Macrophages 23658846    
 Gene ontology annotations for Wasf2
Molecular Function
    protein complex binding GO:0032403 ISO
    protein binding GO:0005515 IPI
    actin binding GO:0003779 IEA
Biological Process
    ameboidal-type cell migration GO:0001667 IMP
    lamellipodium assembly GO:0030032 IMP
    negative regulation of stress fiber assembly GO:0051497 ISO
    Rac protein signal transduction GO:0016601 IMP
    positive regulation of lamellipodium assembly GO:0010592 ISO
    lamellipodium morphogenesis GO:0072673 IMP
    actin filament-based movement GO:0030048 IMP
    megakaryocyte development GO:0035855 IMP
    angiogenesis GO:0001525 IMP
    endocytosis GO:0006897 IDA
    actin cytoskeleton organization GO:0030036 IMP
    movement of cell or subcellular component GO:0006928 IMP
Subcellular Localization
    lamellipodium GO:0030027 ISA
    cytoplasm GO:0005737 IEA
    extracellular exosome GO:0070062 ISO
    early endosome GO:0005769 IDA
    SCAR complex GO:0031209 ISO
    cell-cell junction GO:0005911 IDA
    intracellular GO:0005622 ISA
    cytoskeleton GO:0005856 IEA
    ruffle GO:0001726 IDA
 Experiment description of studies that identified Wasf2 in exosomes
1
Experiment ID 214
ISEV standards
EM
EV Biophysical techniques
EV Cytosolic markers
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors Hassani K, Olivier M.
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name Leishmania-infected-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
2
Experiment ID 215
ISEV standards
EM
EV Biophysical techniques
EV Cytosolic markers
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors Hassani K, Olivier M.
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name LPS-treated-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 216
ISEV standards
EM
EV Biophysical techniques
EV Cytosolic markers
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors Hassani K, Olivier M.
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name Normal-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for Wasf2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 PRPF40A  
Reconstituted Complex Mus musculus
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