Gene description for Wasf2
Gene name WAS protein family, member 2
Gene symbol Wasf2
Other names/aliases AW742646
D4Ertd13e
WAVE2
Species Mus musculus
 Database cross references - Wasf2
ExoCarta ExoCarta_242687
Vesiclepedia VP_242687
Entrez Gene 242687
UniProt Q8BH43  
 Wasf2 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
Macrophages 23658846    
Macrophages 23658846    
Macrophages 23658846    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Wasf2
Molecular Function
    actin binding GO:0003779 IEA
    protein binding GO:0005515 IPI
    SH3 domain binding GO:0017124 IEA
    SH3 domain binding GO:0017124 ISO
    protein kinase A regulatory subunit binding GO:0034237 IBA
    protein kinase A binding GO:0051018 IEA
    protein kinase A binding GO:0051018 ISO
    Arp2/3 complex binding GO:0071933 IBA
Biological Process
    angiogenesis GO:0001525 IMP
    ameboidal-type cell migration GO:0001667 IMP
    endocytosis GO:0006897 IDA
    positive regulation of lamellipodium assembly GO:0010592 IEA
    positive regulation of lamellipodium assembly GO:0010592 ISO
    Rac protein signal transduction GO:0016601 IMP
    lamellipodium assembly GO:0030032 IMP
    actin cytoskeleton organization GO:0030036 IBA
    actin cytoskeleton organization GO:0030036 IMP
    actin filament-based movement GO:0030048 IMP
    megakaryocyte development GO:0035855 IMP
    cell motility GO:0048870 IMP
    negative regulation of stress fiber assembly GO:0051497 IEA
    negative regulation of stress fiber assembly GO:0051497 ISO
    lamellipodium morphogenesis GO:0072673 IMP
    postsynaptic actin cytoskeleton organization GO:0098974 IDA
    postsynaptic actin cytoskeleton organization GO:0098974 IMP
    postsynapse organization GO:0099173 IDA
    postsynapse organization GO:0099173 IMP
    positive regulation of Arp2/3 complex-mediated actin nucleation GO:2000601 IBA
Subcellular Localization
    ruffle GO:0001726 IDA
    intracellular anatomical structure GO:0005622 ISO
    early endosome GO:0005769 IDA
    cytoskeleton GO:0005856 IEA
    cell-cell junction GO:0005911 IDA
    basolateral plasma membrane GO:0016323 IEA
    lamellipodium GO:0030027 IBA
    lamellipodium GO:0030027 IDA
    lamellipodium GO:0030027 ISO
    SCAR complex GO:0031209 IBA
    SCAR complex GO:0031209 IDA
    SCAR complex GO:0031209 ISO
    protein-containing complex GO:0032991 ISO
    synapse GO:0045202 EXP
    synapse GO:0045202 IDA
 Experiment description of studies that identified Wasf2 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 214
MISEV standards
✔
EM
Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors "Hassani K, Olivier M."
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name Leishmania-infected-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 215
MISEV standards
✔
EM
Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors "Hassani K, Olivier M."
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name LPS-treated-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 216
MISEV standards
✔
EM
Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors "Hassani K, Olivier M."
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name Normal-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
5
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Wasf2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Wasf1  
Co-fractionation Mus musculus
2 Nckap1 50884
Co-fractionation Mus musculus
3 Atg16l1  
Affinity Capture-MS Mus musculus
4 Cyfip1 20430
Co-fractionation Mus musculus
5 Cyfip2 76884
Co-fractionation Mus musculus
6 Prpf40a  
Reconstituted Complex Mus musculus
7 Abi1 11308
Affinity Capture-MS Mus musculus
8 Shank3  
Affinity Capture-MS Mus musculus
9 Abi2  
Co-fractionation Mus musculus
10 Tcerg1  
Co-fractionation Mus musculus
11 Golga4  
Co-fractionation Mus musculus
12 Apbb1ip  
Co-fractionation Mus musculus
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