Gene description for Dctn1
Gene name dynactin 1
Gene symbol Dctn1
Other names/aliases AL022633
Glued
p150
p150
Species Mus musculus
 Database cross references - Dctn1
ExoCarta ExoCarta_13191
Vesiclepedia VP_13191
Entrez Gene 13191
UniProt O08788  
 Dctn1 identified in exosomes derived from the following tissue/cell type
Colon cancer cells 37309723    
Fibroblasts 23260141    
Pancreatic cells 19351151    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Dctn1
Molecular Function
    protein binding GO:0005515 IPI
    microtubule binding GO:0008017 IDA
    microtubule binding GO:0008017 ISO
    tubulin binding GO:0015631 ISO
    protein kinase binding GO:0019901 IEA
    protein kinase binding GO:0019901 ISO
    identical protein binding GO:0042802 ISO
    molecular adaptor activity GO:0060090 ISO
Biological Process
    establishment of mitotic spindle orientation GO:0000132 IBA
    establishment of mitotic spindle orientation GO:0000132 ISO
    establishment of mitotic spindle orientation GO:0000132 ISS
    nuclear migration GO:0007097 IBA
    neuromuscular junction development GO:0007528 IEA
    neuromuscular junction development GO:0007528 ISO
    centriole-centriole cohesion GO:0010457 ISO
    centriole-centriole cohesion GO:0010457 ISS
    ventral spinal cord development GO:0021517 IEA
    ventral spinal cord development GO:0021517 ISO
    positive regulation of microtubule polymerization GO:0031116 ISO
    positive regulation of microtubule polymerization GO:0031116 ISS
    cytoplasmic microtubule organization GO:0031122 ISS
    melanosome transport GO:0032402 IMP
    microtubule anchoring at centrosome GO:0034454 ISO
    microtubule anchoring at centrosome GO:0034454 ISS
    retrograde transport, endosome to Golgi GO:0042147 IEA
    retrograde transport, endosome to Golgi GO:0042147 ISO
    neuromuscular process GO:0050905 IEA
    neuromuscular process GO:0050905 ISO
    nuclear membrane disassembly GO:0051081 ISO
    nuclear membrane disassembly GO:0051081 ISS
    cell division GO:0051301 IEA
    regulation of mitotic spindle organization GO:0060236 ISO
    regulation of mitotic spindle organization GO:0060236 ISS
    motor behavior GO:0061744 IEA
    motor behavior GO:0061744 ISO
    neuron cellular homeostasis GO:0070050 IEA
    neuron cellular homeostasis GO:0070050 ISO
    positive regulation of microtubule nucleation GO:0090063 ISO
    positive regulation of microtubule nucleation GO:0090063 ISS
    positive regulation of intracellular protein transport GO:0090316 ISS
    positive regulation of neuromuscular junction development GO:1904398 IEA
    positive regulation of neuromuscular junction development GO:1904398 ISO
    non-motile cilium assembly GO:1905515 ISO
    non-motile cilium assembly GO:1905515 ISS
    neuron projection maintenance GO:1990535 IEA
    neuron projection maintenance GO:1990535 ISO
Subcellular Localization
    kinetochore GO:0000776 IBA
    kinetochore GO:0000776 ISO
    kinetochore GO:0000776 ISS
    spindle pole GO:0000922 IBA
    spindle pole GO:0000922 IDA
    spindle pole GO:0000922 ISO
    nuclear envelope GO:0005635 ISO
    nuclear envelope GO:0005635 ISS
    cytoplasm GO:0005737 ISO
    centrosome GO:0005813 ISO
    centrosome GO:0005813 ISS
    centriole GO:0005814 IDA
    centriole GO:0005814 ISO
    spindle GO:0005819 ISO
    spindle GO:0005819 ISS
    cytosol GO:0005829 IEA
    cytoplasmic dynein complex GO:0005868 ISO
    microtubule GO:0005874 ISO
    microtubule associated complex GO:0005875 IBA
    microtubule associated complex GO:0005875 ISO
    cell cortex GO:0005938 ISO
    cell cortex GO:0005938 ISO
    cell cortex GO:0005938 ISS
    microtubule cytoskeleton GO:0015630 ISO
    dynein complex GO:0030286 IEA
    axon GO:0030424 IBA
    retromer complex GO:0030904 IEA
    retromer complex GO:0030904 ISO
    cell leading edge GO:0031252 IDA
    protein-containing complex GO:0032991 IDA
    microtubule plus-end GO:0035371 IDA
    microtubule plus-end GO:0035371 ISO
    ciliary basal body GO:0036064 IDA
    neuron projection GO:0043005 IEA
    neuron projection GO:0043005 ISO
    neuronal cell body GO:0043025 IEA
    neuronal cell body GO:0043025 ISO
    intercellular bridge GO:0045171 IEA
    intercellular bridge GO:0045171 ISO
    mitotic spindle GO:0072686 IEA
    mitotic spindle GO:0072686 ISO
    cell cortex region GO:0099738 ISO
    cell cortex region GO:0099738 ISS
    centriolar subdistal appendage GO:0120103 IEA
 Experiment description of studies that identified Dctn1 in exosomes
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S.
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 210
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|FLOT1
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23260141    
Organism Mus musculus
Experiment description Exosomes Mediate Stromal Mobilization of Autocrine Wnt-PCP Signaling in Breast Cancer Cell Migration.
Authors Luga V, Zhang L, Viloria-Petit AM, Ogunjimi AA, Inanlou MR, Chiu E, Buchanan M, Hosein AN, Basik M, Wrana JL.
Journal name Cell
Publication year 2012
Sample Fibroblasts
Sample name Normal-Fibroblasts (L cells)
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 188
MISEV standards
✔
EM
Biophysical techniques
✔
GAPDH|UCHL1|HSP90
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19351151    
Organism Mus musculus
Experiment description Characterization of vesicles secreted from insulinoma NIT-1 cells.
Authors Lee HS, Jeong J, Lee KJ.
Journal name J Proteome Res
Publication year 2009
Sample Pancreatic cells
Sample name Pancreatic beta cell (NIT-1)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S.
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Dctn1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 CDK1 983
Affinity Capture-MS Homo sapiens
2 KIF16B 55614
Affinity Capture-MS Homo sapiens
3 DCTN3 11258
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
4 Mycbp2 105689
Affinity Capture-MS Mus musculus
5 ANKLE2 23141
Affinity Capture-MS Homo sapiens
6 Snx6 72183
Co-fractionation Mus musculus
7 UQCRQ 27089
Affinity Capture-MS Homo sapiens
8 Bicd2  
Co-fractionation Mus musculus
9 MRPS12  
Affinity Capture-MS Homo sapiens
10 Apc  
Co-fractionation Mus musculus
11 DCTN6 10671
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
12 Htt  
Affinity Capture-Western Mus musculus
Affinity Capture-MS Mus musculus
13 Nup85  
Co-fractionation Mus musculus
14 CEP250 11190
Affinity Capture-MS Homo sapiens
15 Atxn1  
Affinity Capture-MS Mus musculus
16 Kctd13  
Affinity Capture-MS Mus musculus
17 Shank3  
Affinity Capture-MS Mus musculus
Affinity Capture-MS Mus musculus
18 CAPZA2 830
Affinity Capture-MS Homo sapiens
19 CAPZA1 829
Affinity Capture-MS Homo sapiens
20 DCTN2 10540
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
21 PTDSS1 9791
Affinity Capture-MS Homo sapiens
22 DCTN5 84516
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
23 Usp48  
Affinity Capture-MS Mus musculus
24 TRIP11 9321
Affinity Capture-MS Homo sapiens
25 ACTR1B 10120
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
26 Ndel1  
Co-purification Mus musculus
Co-fractionation Mus musculus
27 Clasp1  
Co-fractionation Mus musculus
28 Mettl21e  
Affinity Capture-MS Mus musculus
29 CAPZB 832
Affinity Capture-MS Homo sapiens
30 Cyfip1 20430
Affinity Capture-MS Mus musculus
31 Uso1 56041
Co-fractionation Mus musculus
32 DYNC1LI1 51143
Affinity Capture-MS Homo sapiens
33 DCTN4 51164
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
34 DYNLRB1 83658
Affinity Capture-MS Homo sapiens
35 DCTN1 1639
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
36 Snx2 67804
Co-fractionation Mus musculus
37 Snx5 69178
Co-fractionation Mus musculus
38 ACTR1A 10121
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
39 Eed  
Affinity Capture-MS Mus musculus
40 Ndn  
Two-hybrid Mus musculus
41 Snx1  
Co-fractionation Mus musculus
42 ACTR10 55860
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
43 Snx3 54198
Co-fractionation Mus musculus
44 CEP83  
Affinity Capture-MS Homo sapiens
45 DERL1 79139
Affinity Capture-MS Homo sapiens
46 Snx27 76742
Co-fractionation Mus musculus
View the network image/svg+xml
 Pathways in which Dctn1 is involved
PathwayEvidenceSource
Adaptive Immune System IEA Reactome
Anchoring of the basal body to the plasma membrane IEA Reactome
Asparagine N-linked glycosylation IEA Reactome
AURKA Activation by TPX2 IEA Reactome
Cell Cycle IEA Reactome
Cell Cycle, Mitotic IEA Reactome
Cellular responses to stimuli IEA Reactome
Cellular responses to stress IEA Reactome
Centrosome maturation IEA Reactome
Cilium Assembly IEA Reactome
COPI-independent Golgi-to-ER retrograde traffic IEA Reactome
COPI-mediated anterograde transport IEA Reactome
ER to Golgi Anterograde Transport IEA Reactome
G2/M Transition IEA Reactome
Golgi-to-ER retrograde transport IEA Reactome
HSP90 chaperone cycle for steroid hormone receptors (SHR) in the presence of ligand IEA Reactome
Immune System IEA Reactome
Intra-Golgi and retrograde Golgi-to-ER traffic IEA Reactome
Loss of Nlp from mitotic centrosomes IEA Reactome
Loss of proteins required for interphase microtubule organization from the centrosome IEA Reactome
M Phase IEA Reactome
Membrane Trafficking IEA Reactome
Metabolism of proteins IEA Reactome
MHC class II antigen presentation IEA Reactome
Mitotic G2-G2/M phases IEA Reactome
Mitotic Prometaphase IEA Reactome
Organelle biogenesis and maintenance IEA Reactome
Post-translational protein modification IEA Reactome
Recruitment of mitotic centrosome proteins and complexes IEA Reactome
Recruitment of NuMA to mitotic centrosomes IEA Reactome
Regulation of PLK1 Activity at G2/M Transition IEA Reactome
Transport to the Golgi and subsequent modification IEA Reactome
Vesicle-mediated transport IEA Reactome





Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here