Gene description for Dctn1
Gene name dynactin 1
Gene symbol Dctn1
Other names/aliases AL022633
Glued
p150
p150
Species Mus musculus
 Database cross references - Dctn1
ExoCarta ExoCarta_13191
Entrez Gene 13191
UniProt O08788  
 Dctn1 identified in exosomes derived from the following tissue/cell type
Fibroblasts 23260141    
Pancreatic cells 19351151    
 Gene ontology annotations for Dctn1
Molecular Function
    protein binding GO:0005515 IPI
    dynein binding GO:0045502 IEA
    motor activity GO:0003774 IEA
Biological Process
    retrograde transport, endosome to Golgi GO:0042147 ISO
    transport GO:0006810 IEA
    microtubule-based transport GO:0010970 IEA
    melanosome transport GO:0032402 IMP
Subcellular Localization
    cytoplasm GO:0005737 IEA
    protein complex GO:0043234 IDA
    cell GO:0005623 IMP
    cytoskeleton GO:0005856 IEA
    cytoplasmic dynein complex GO:0005868 ISO
    retromer complex GO:0030904 ISO
    kinetochore GO:0000776 ISO
    dynactin complex GO:0005869 IEA
    spindle pole GO:0000922 ISO
    membrane GO:0016020 ISO
    microtubule GO:0005874 ISO
    cell leading edge GO:0031252 IDA
    dynein complex GO:0030286 IEA
    centrosome GO:0005813 ISO
 Experiment description of studies that identified Dctn1 in exosomes
1
Experiment ID 210
ISEV standards
EM
EV Biophysical techniques
EV Cytosolic markers
CD81|FLOT1
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23260141    
Organism Mus musculus
Experiment description Exosomes Mediate Stromal Mobilization of Autocrine Wnt-PCP Signaling in Breast Cancer Cell Migration.
Authors Luga V, Zhang L, Viloria-Petit AM, Ogunjimi AA, Inanlou MR, Chiu E, Buchanan M, Hosein AN, Basik M, Wrana JL.
Journal name Cell
Publication year 2012
Sample Fibroblasts
Sample name Normal-Fibroblasts (L cells)
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
2
Experiment ID 188
ISEV standards
EM
EV Biophysical techniques
GAPDH|UCHL1|HSP90
EV Cytosolic markers
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19351151    
Organism Mus musculus
Experiment description Characterization of vesicles secreted from insulinoma NIT-1 cells.
Authors Lee HS, Jeong J, Lee KJ.
Journal name J Proteome Res
Publication year 2009
Sample Pancreatic cells
Sample name Pancreatic beta cell (NIT-1)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for Dctn1
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.



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