Gene description for Snx5
Gene name sorting nexin 5
Gene symbol Snx5
Other names/aliases 0910001N05Rik
1810032P22Rik
AU019504
D2Ertd52e
Species Mus musculus
 Database cross references - Snx5
ExoCarta ExoCarta_69178
Vesiclepedia VP_69178
Entrez Gene 69178
UniProt Q9D8U8  
 Snx5 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Snx5
Molecular Function
    protein binding GO:0005515 IPI
    phosphatidylinositol-5-phosphate binding GO:0010314 IEA
    phosphatidylinositol-5-phosphate binding GO:0010314 ISO
    D1 dopamine receptor binding GO:0031748 IEA
    D1 dopamine receptor binding GO:0031748 ISO
    dynactin binding GO:0034452 IBA
    dynactin binding GO:0034452 ISO
    dynactin binding GO:0034452 ISS
    phosphatidylinositol binding GO:0035091 IBA
    phosphatidylinositol binding GO:0035091 ISO
    phosphatidylinositol binding GO:0035091 ISS
    phosphatidylinositol-4-phosphate binding GO:0070273 IEA
    phosphatidylinositol-4-phosphate binding GO:0070273 ISO
    phosphatidylinositol-3,5-bisphosphate binding GO:0080025 IEA
    phosphatidylinositol-3,5-bisphosphate binding GO:0080025 ISO
Biological Process
    intracellular protein transport GO:0006886 IEA
    pinocytosis GO:0006907 IBA
    pinocytosis GO:0006907 ISO
    pinocytosis GO:0006907 ISS
    epidermal growth factor catabolic process GO:0007174 IEA
    epidermal growth factor catabolic process GO:0007174 ISO
    retrograde transport, endosome to Golgi GO:0042147 IBA
    retrograde transport, endosome to Golgi GO:0042147 IEA
    retrograde transport, endosome to Golgi GO:0042147 ISO
    negative regulation of blood pressure GO:0045776 IEA
    negative regulation of blood pressure GO:0045776 ISO
    positive regulation of DNA-templated transcription GO:0045893 IEA
    positive regulation of DNA-templated transcription GO:0045893 ISO
    positive regulation of insulin receptor signaling pathway GO:0046628 IEA
    positive regulation of insulin receptor signaling pathway GO:0046628 ISO
Subcellular Localization
    ruffle GO:0001726 IEA
    phagocytic cup GO:0001891 IEA
    endosome GO:0005768 IBA
    endosome GO:0005768 ISO
    cytosol GO:0005829 IEA
    cytosol GO:0005829 ISO
    brush border GO:0005903 IEA
    brush border GO:0005903 ISO
    cytoplasmic side of plasma membrane GO:0009898 ISO
    cytoplasmic side of plasma membrane GO:0009898 ISS
    retromer complex GO:0030904 ISO
    retromer complex GO:0030904 ISS
    intracellular membrane-bounded organelle GO:0043231 ISO
    perinuclear region of cytoplasm GO:0048471 IEA
    perinuclear region of cytoplasm GO:0048471 ISO
    macropinocytic cup GO:0070685 ISO
    macropinocytic cup GO:0070685 ISS
    tubular endosome GO:0097422 ISO
    tubular endosome GO:0097422 ISS
    cytoplasmic side of early endosome membrane GO:0098559 ISO
    cytoplasmic side of early endosome membrane GO:0098559 ISS
 Experiment description of studies that identified Snx5 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Snx5
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Arfip2  
Co-fractionation Mus musculus
2 Snx1  
Co-fractionation Mus musculus
3 Snx2 67804
Co-fractionation Mus musculus
4 Tmem173  
Proximity Label-MS Mus musculus
5 Lamtor5 68576
Co-fractionation Mus musculus
6 Pip5k1c  
Co-fractionation Mus musculus
7 Vps29 56433
Co-fractionation Mus musculus
8 Dnajb11  
Co-fractionation Mus musculus
9 Snx6 72183
Co-fractionation Mus musculus
10 Sars 20226
Co-fractionation Mus musculus
11 Sh3glb2  
Co-fractionation Mus musculus
12 Vps26a 30930
Co-fractionation Mus musculus
13 Cdc37 12539
Co-fractionation Mus musculus
14 Zyx  
Co-fractionation Mus musculus
15 Dctn1 13191
Co-fractionation Mus musculus
16 Map1s  
Co-fractionation Mus musculus
17 Snx27 76742
Co-fractionation Mus musculus
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