ExoCarta: Gene summary

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Gene description for DCTN6
Gene name	dynactin 6
Gene symbol	DCTN6
Other names/aliases	WS-3 WS3 p27
Species	Homo sapiens
Database cross references - DCTN6
ExoCarta	ExoCarta_10671
Vesiclepedia	VP_10671
Entrez Gene	10671
HGNC	16964
MIM	612963
UniProt	O00399
DCTN6 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells	36408942
Bone marrow mesenchymal stem cells	36408942
Bone marrow mesenchymal stem cells	36408942
Bone marrow mesenchymal stem cells	36408942

Gene ontology annotations for DCTN6

Molecular Function
	dynein complex binding	GO:0070840	IBA
Biological Process
	mitotic spindle organization	GO:0007052	IBA
Subcellular Localization
	kinetochore	GO:0000776	IEA
	centrosome	GO:0005813	IDA
	cytosol	GO:0005829	TAS
	dynactin complex	GO:0005869	IBA

Experiment description of studies that identified DCTN6 in sEVs

Experiment ID

489

MISEV standards
✔ EM	Biophysical techniques
✔ Cd9\|Cd81\|Cd63\|Gapdh\|Sdcbp\|Lamp1\|Aqp1\|Rab5a\|Icam1\|Cd82\|Itga2b\|Tsg101\|Lamp2\|Rab35\|Flot1\|Flot2\|Cd151\|Rab5b\|Tfrc\|Uchl1	Enriched markers
✔ Canx	Negative markers
✔ NTA	Particle analysis

Identified molecule

Protein

Identification method

Mass spectrometry

PubMed ID

36408942

Organism

Rattus norvegicus

Experiment description

Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells

Authors

"Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."

Journal name

Proteomics

Publication year

2023

Sample

Bone marrow mesenchymal stem cells

Sample name

BMSC - Passage 6

Isolation/purification methods

Differential centrifugation
Filtration
Ultracentrifugation

Flotation density

Molecules identified in the study

Protein

Methods used in the study

Western blotting
Mass spectometry

Experiment ID

490

MISEV standards
✔ EM	Biophysical techniques
✔ Cd9\|Cd81\|Cd63\|Gapdh\|Sdcbp\|Lamp1\|Aqp1\|Rab5a\|Icam1\|Cd82\|Itga2b\|Tsg101\|Lamp2\|Rab35\|Flot1\|Flot2\|Cd151\|Rab5b\|Tfrc\|Uchl1	Enriched markers
✔ Canx	Negative markers
✔ NTA	Particle analysis

Identified molecule

Protein

Identification method

Mass spectrometry

PubMed ID

36408942

Organism

Rattus norvegicus

Experiment description

Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells

Authors

"Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."

Journal name

Proteomics

Publication year

2023

Sample

Bone marrow mesenchymal stem cells

Sample name

BMSC - Passage 7

Isolation/purification methods

Differential centrifugation
Filtration
Ultracentrifugation

Flotation density

Molecules identified in the study

Protein

Methods used in the study

Western blotting
Mass spectometry

Experiment ID

491

MISEV standards
✔ EM	Biophysical techniques
✔ Cd9\|Cd81\|Cd63\|Gapdh\|Sdcbp\|Lamp1\|Aqp1\|Rab5a\|Icam1\|Cd82\|Itga2b\|Tsg101\|Lamp2\|Rab35\|Flot1\|Flot2\|Cd151\|Rab5b\|Tfrc\|Uchl1	Enriched markers
✔ Canx	Negative markers
✔ NTA	Particle analysis

Identified molecule

Protein

Identification method

Mass spectrometry

PubMed ID

36408942

Organism

Rattus norvegicus

Experiment description

Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells

Authors

"Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."

Journal name

Proteomics

Publication year

2023

Sample

Bone marrow mesenchymal stem cells

Sample name

BMSC - Passage 8

Isolation/purification methods

Differential centrifugation
Filtration
Ultracentrifugation

Flotation density

Molecules identified in the study

Protein

Methods used in the study

Western blotting
Mass spectometry

Experiment ID

492

MISEV standards
✔ EM	Biophysical techniques
✔ Cd9\|Cd81\|Cd63\|Gapdh\|Sdcbp\|Lamp1\|Aqp1\|Rab5a\|Icam1\|Cd82\|Itga2b\|Tsg101\|Lamp2\|Rab35\|Flot1\|Flot2\|Cd151\|Rab5b\|Tfrc\|Uchl1	Enriched markers
✔ Canx	Negative markers
✔ NTA	Particle analysis

Identified molecule

Protein

Identification method

Mass spectrometry

PubMed ID

36408942

Organism

Rattus norvegicus

Experiment description

Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells

Authors

"Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."

Journal name

Proteomics

Publication year

2023

Sample

Bone marrow mesenchymal stem cells

Sample name

BMSC - Passage 9

Isolation/purification methods

Differential centrifugation
Filtration
Ultracentrifugation

Flotation density

Molecules identified in the study

Protein

Methods used in the study

Western blotting
Mass spectometry

Protein-protein interactions for DCTN6

Protein Interactor

ExoCarta ID

Identification method

PubMed

Species

CAPZA1

829

Affinity Capture-MS

Homo sapiens

RPSA

3921

Two-hybrid

Homo sapiens

CALML3

810

Affinity Capture-MS

Homo sapiens

RIT1

6016

Negative Genetic

Homo sapiens

Dctn5

Affinity Capture-MS

Mus musculus

RPS6KA2

Affinity Capture-MS

Homo sapiens

DCTN3

11258

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

SEPT14

Affinity Capture-MS

Homo sapiens

HSPB2

Two-hybrid

Homo sapiens

PDGFD

80310

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

C9orf24

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

AVIL

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

Dctn1

13191

Affinity Capture-MS		Mus musculus
Affinity Capture-MS		Mus musculus

N4BP2L1

Affinity Capture-MS

Homo sapiens

CXCL14

Affinity Capture-MS

Homo sapiens

RPS19BP1

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

C1orf106

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

UQCRH

7388

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

CCDC120

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

PMEPA1

56937

Two-hybrid		Homo sapiens
Reconstituted Complex		Homo sapiens

ABRA

Affinity Capture-MS

Homo sapiens

MAPK8IP3

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

Capza1

12340

Affinity Capture-MS

Mus musculus

FOXA1

Affinity Capture-MS

Homo sapiens

TIMM10

Affinity Capture-MS

Homo sapiens

CAPZA2

830

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

ACTR1B

10120

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

DCTN2

10540

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

RIC8A

60626

Affinity Capture-MS

Homo sapiens

CSPP1

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

DCTN5

84516

Affinity Capture-MS		Homo sapiens
Co-fractionation		Homo sapiens
Affinity Capture-MS		Homo sapiens

KLC3

Affinity Capture-MS

Homo sapiens

C16orf45

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

CCDC102B

Affinity Capture-MS

Homo sapiens

CCL4L1

Affinity Capture-MS

Homo sapiens

APP

351

Reconstituted Complex

Homo sapiens

DYNC1I2

1781

Proximity Label-MS

Homo sapiens

MAPRE1

22919

Affinity Capture-MS

Homo sapiens

Dctn2

69654

Affinity Capture-MS		Mus musculus
Affinity Capture-MS		Mus musculus

CAPZB

832

Co-fractionation		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

CPVL

54504

Affinity Capture-MS

Homo sapiens

GSN

2934

Affinity Capture-MS

Homo sapiens

C12orf49

Negative Genetic

Homo sapiens

Actr1b

226977

Affinity Capture-MS

Mus musculus

IL17B

27190

Affinity Capture-MS

Homo sapiens

Dctn3

Affinity Capture-MS		Mus musculus
Affinity Capture-MS		Mus musculus

ERRFI1

54206

Two-hybrid

Homo sapiens

LOC554223

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

DCTN4

51164

Co-fractionation		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

RAB11A

8766

Proximity Label-MS

Homo sapiens

ZBTB43

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

AKR7A2

8574

Affinity Capture-MS

Homo sapiens

DEFB127

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

DCTN1

1639

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

C4BPB

725

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

CCL19

Affinity Capture-MS

Homo sapiens

Actr1a

54130

Affinity Capture-MS

Mus musculus

KRAS

3845

Negative Genetic

Homo sapiens

ACTR1A

10121

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

MIS18A

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

NCOA3

Two-hybrid

Homo sapiens

ACTR10

55860

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

ORC6

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

MED22

Affinity Capture-MS

Homo sapiens

KLHL2

11275

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

Dctn4

Affinity Capture-MS

Mus musculus

GPS2

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

View the network

Pathways in which DCTN6 is involved

Pathway	Evidence	Source
Adaptive Immune System	TAS	Reactome
Asparagine N-linked glycosylation	TAS	Reactome
Cellular responses to stimuli	TAS	Reactome
Cellular responses to stress	TAS	Reactome
COPI-independent Golgi-to-ER retrograde traffic	TAS	Reactome
COPI-mediated anterograde transport	TAS	Reactome
ER to Golgi Anterograde Transport	TAS	Reactome
Golgi-to-ER retrograde transport	TAS	Reactome
HSP90 chaperone cycle for steroid hormone receptors (SHR) in the presence of ligand	TAS	Reactome
Immune System	TAS	Reactome
Intra-Golgi and retrograde Golgi-to-ER traffic	TAS	Reactome
Membrane Trafficking	TAS	Reactome
Metabolism of proteins	TAS	Reactome
MHC class II antigen presentation	TAS	Reactome
Post-translational protein modification	TAS	Reactome
Transport to the Golgi and subsequent modification	TAS	Reactome
Vesicle-mediated transport	TAS	Reactome

Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here