Gene description for DCTN4
Gene name dynactin 4 (p62)
Gene symbol DCTN4
Other names/aliases DYN4
P62
Species Homo sapiens
 Database cross references - DCTN4
ExoCarta ExoCarta_51164
Vesiclepedia VP_51164
Entrez Gene 51164
HGNC 15518
MIM 614758
UniProt Q9UJW0  
 DCTN4 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Chondrocytes 35931686    
Chondrocytes 35931686    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Mesenchymal stem cells 36408942    
 Gene ontology annotations for DCTN4
Molecular Function
    protein binding GO:0005515 IPI
Subcellular Localization
    kinetochore GO:0000776 IEA
    spindle pole GO:0000922 IEA
    stress fiber GO:0001725 IEA
    nucleus GO:0005634 TAS
    cytoplasm GO:0005737 TAS
    centrosome GO:0005813 IDA
    cytosol GO:0005829 TAS
    cytoplasmic dynein complex GO:0005868 IEA
    dynactin complex GO:0005869 IBA
    focal adhesion GO:0005925 IEA
    cell cortex GO:0005938 IEA
    sarcomere GO:0030017 IEA
 Experiment description of studies that identified DCTN4 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 497
MISEV standards
✘ Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
6
Experiment ID 498
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
7
Experiment ID 363
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
8
Experiment ID 364
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
9
Experiment ID 365
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
10
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for DCTN4
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 RIT1 6016
Negative Genetic Homo sapiens
2 CLIP1 6249
Affinity Capture-MS Homo sapiens
3 BRDT  
Affinity Capture-MS Homo sapiens
4 ABRA  
Affinity Capture-MS Homo sapiens
5 DCTN6 10671
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
6 TNPO1 3842
Affinity Capture-MS Homo sapiens
7 RPS19BP1  
Affinity Capture-MS Homo sapiens
8 DYNC1I2 1781
Proximity Label-MS Homo sapiens
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
9 BTF3 689
Affinity Capture-MS Homo sapiens
10 ACTC1 70
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
11 DYNC1I1  
Proximity Label-MS Homo sapiens
12 BRD2  
Affinity Capture-MS Homo sapiens
13 MCCC1 56922
Proximity Label-MS Homo sapiens
14 RIC8A 60626
Affinity Capture-MS Homo sapiens
15 Actb 11461
Affinity Capture-MS Mus musculus
16 DEFB127  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
17 CAPZB 832
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Proximity Label-MS Homo sapiens
18 DCTN4 51164
Proximity Label-MS Homo sapiens
Proximity Label-MS Homo sapiens
19 CALD1 800
Proximity Label-MS Homo sapiens
20 ACTR1A 10121
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
Proximity Label-MS Homo sapiens
21 HSPH1 10808
Proximity Label-MS Homo sapiens
22 CALCOCO2  
Two-hybrid Homo sapiens
23 SMC2 10592
Co-fractionation Homo sapiens
24 MTUS2 23281
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
25 DYNLL1 8655
Affinity Capture-MS Homo sapiens
26 MED22  
Affinity Capture-MS Homo sapiens
27 ZC3HAV1 56829
Proximity Label-MS Homo sapiens
28 DNAJC7 7266
Proximity Label-MS Homo sapiens
29 Dctn5  
Affinity Capture-MS Mus musculus
30 TIMM50 92609
Proximity Label-MS Homo sapiens
31 Lima1  
Affinity Capture-MS Mus musculus
32 Dctn1 13191
Affinity Capture-MS Mus musculus
Affinity Capture-MS Mus musculus
33 N4BP2L1  
Affinity Capture-MS Homo sapiens
34 MAPK8IP3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
35 FOXA1  
Affinity Capture-MS Homo sapiens
36 CAPZA1 829
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Proximity Label-MS Homo sapiens
37 PARK2  
Affinity Capture-MS Homo sapiens
38 DCTN5 84516
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
39 ATP7B 540
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
40 MAPRE1 22919
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
41 GOLGA2 2801
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
42 ACACA 31
Proximity Label-MS Homo sapiens
43 PCCA 5095
Proximity Label-MS Homo sapiens
44 NTRK1 4914
Affinity Capture-MS Homo sapiens
45 DCTN1 1639
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
46 C4BPB 725
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
47 ACTB 60
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
48 MYH10 4628
Co-fractionation Homo sapiens
49 UQCRH 7388
Affinity Capture-MS Homo sapiens
50 ACTR10 55860
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
Two-hybrid Homo sapiens
Co-fractionation Homo sapiens
Proximity Label-MS Homo sapiens
51 ACTG1 71
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
52 HLCS  
Proximity Label-MS Homo sapiens
53 GPS2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
54 POLR2B 5431
Proximity Label-MS Homo sapiens
55 AKAP10 11216
Co-fractionation Homo sapiens
56 DCTN3 11258
Proximity Label-MS Homo sapiens
57 NASP 4678
Co-fractionation Homo sapiens
58 EIF4G1 1981
Co-fractionation Homo sapiens
59 MIS18A  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
60 TUBB4B 10383
Affinity Capture-MS Homo sapiens
61 MCM4 4173
Co-fractionation Homo sapiens
62 DUSP13  
Affinity Capture-MS Homo sapiens
63 Dctn2 69654
Affinity Capture-MS Mus musculus
Affinity Capture-MS Mus musculus
64 PRMT5 10419
Co-fractionation Homo sapiens
65 NIN 51199
Proximity Label-MS Homo sapiens
66 C16orf45  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
67 BICD1 636
Proximity Label-MS Homo sapiens
68 SEH1L 81929
Co-fractionation Homo sapiens
69 KIAA0368 23392
Proximity Label-MS Homo sapiens
70 CCL4L1  
Affinity Capture-MS Homo sapiens
71 Cep76  
Affinity Capture-MS Mus musculus
72 KLHL2 11275
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
73 GSN 2934
Affinity Capture-MS Homo sapiens
74 PAFAH1B1 5048
Proximity Label-MS Homo sapiens
75 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
76 PRKCSH 5589
Co-fractionation Homo sapiens
77 PDGFD 80310
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
78 Capza1 12340
Affinity Capture-MS Mus musculus
79 ALDH1B1 219
Proximity Label-MS Homo sapiens
80 CAPZA2 830
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
81 ACTR1B 10120
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
Proximity Label-MS Homo sapiens
82 DCTN2 10540
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
Proximity Label-MS Homo sapiens
83 CSPP1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
84 PNMA1 9240
Two-hybrid Homo sapiens
85 SHMT2 6472
Proximity Label-MS Homo sapiens
86 DDX58 23586
Affinity Capture-RNA Homo sapiens
87 DYNC1H1 1778
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
88 CLASP1 23332
Affinity Capture-MS Homo sapiens
89 Actr1b 226977
Affinity Capture-MS Mus musculus
90 Dctn3  
Affinity Capture-MS Mus musculus
Affinity Capture-MS Mus musculus
91 RAB7A 7879
Proximity Label-MS Homo sapiens
92 PCCB 5096
Proximity Label-MS Homo sapiens
93 AKR7A2 8574
Affinity Capture-MS Homo sapiens
94 RPL28 6158
Proximity Label-MS Homo sapiens
95 Actr1a 54130
Affinity Capture-MS Mus musculus
96 KRAS 3845
Negative Genetic Homo sapiens
97 C9orf72  
Affinity Capture-MS Homo sapiens
98 DYNC1LI1 51143
Affinity Capture-MS Homo sapiens
View the network image/svg+xml



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