Gene description for RIC8A
Gene name RIC8 guanine nucleotide exchange factor A
Gene symbol RIC8A
Other names/aliases RIC8
Species Homo sapiens
 Database cross references - RIC8A
ExoCarta ExoCarta_60626
Entrez Gene 60626
HGNC 29550
MIM 609146
UniProt Q9NPQ8  
 RIC8A identified in exosomes derived from the following tissue/cell type
Hepatocellular carcinoma cells 26054723    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Thymus 23844026    
 Gene ontology annotations for RIC8A
Molecular Function
    G-protein alpha-subunit binding GO:0001965 IBA
    guanyl-nucleotide exchange factor activity GO:0005085 IBA
    protein binding GO:0005515 IPI
    GTPase activator activity GO:0005096 IBA
Biological Process
    adenylate cyclase-inhibiting G-protein coupled receptor signaling pathway GO:0007193 IEA
    cell migration involved in gastrulation GO:0042074 IEA
    positive regulation of GTPase activity GO:0043547 IBA
    vasculature development GO:0001944 IEA
    in utero embryonic development GO:0001701 IEA
    visual learning GO:0008542 IEA
    G-protein coupled receptor signaling pathway GO:0007186 IBA
    cell-cell adhesion involved in gastrulation GO:0070586 IEA
    basement membrane organization GO:0071711 IEA
Subcellular Localization
    cytoplasm GO:0005737 ISS
    plasma membrane GO:0005886 ISS
 Experiment description of studies that identified RIC8A in exosomes
1
Experiment ID 235
ISEV standards
EM
EV Biophysical techniques
TSG101|Alix|HSC70|GAPDH
EV Cytosolic markers
EV Membrane markers
HSP90B1
EV Negative markers
qNano
EV Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
2
Experiment ID 211
ISEV standards
EM
EV Biophysical techniques
TSG101|Alix
EV Cytosolic markers
EpCAM|TFRC
EV Membrane markers
cytochrome c|GOLGA2
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 212
ISEV standards
CEM
EV Biophysical techniques
TSG101|Alix
EV Cytosolic markers
EpCAM|TFRC
EV Membrane markers
Cytochrome C|GOLGA2
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 217
ISEV standards
EM
EV Biophysical techniques
TSG101
EV Cytosolic markers
CD81|CD9|CD63
EV Membrane markers
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O.
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for RIC8A
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 TUBB2A 7280
Two-hybrid Homo sapiens
2 MAPK8IP3  
Two-hybrid Homo sapiens
3 GNA13 10672
Invitro Homo sapiens
Two-hybrid Homo sapiens
4 ZNF585B  
Two-hybrid Homo sapiens
5 TERF1 7013
Two-hybrid Homo sapiens
6 GNAQ 2776
Two-hybrid Homo sapiens
Invitro Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
7 UBQLN1 29979
Two-hybrid Homo sapiens
8 GNAI2 2771
Invitro Homo sapiens
Two-hybrid Homo sapiens
9 GNAO1 2775
Invitro Homo sapiens
Two-hybrid Homo sapiens
10 GNAS 2778
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
11 GNAI3 2773
Invitro Homo sapiens
Two-hybrid Homo sapiens
12 GNAI1 2770
Invitro Homo sapiens
Two-hybrid Homo sapiens
View the network image/svg+xml
 Pathways in which RIC8A is involved
No pathways found





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