Gene description for SPAG5
Gene name sperm associated antigen 5
Gene symbol SPAG5
Other names/aliases DEEPEST
MAP126
hMAP126
Species Homo sapiens
 Database cross references - SPAG5
ExoCarta ExoCarta_10615
Entrez Gene 10615
HGNC 13452
MIM 615562
UniProt Q96R06  
 SPAG5 identified in exosomes derived from the following tissue/cell type
Urine 21595033    
Urine 21595033    
Urine 21595033    
 Gene ontology annotations for SPAG5
Molecular Function
    protein binding GO:0005515 IPI
Biological Process
    chromosome segregation GO:0007059 IMP
    mitotic sister chromatid segregation GO:0000070 IMP
    cell division GO:0051301 IEA
    regulation of attachment of spindle microtubules to kinetochore GO:0051988 IMP
    spindle organization GO:0007051 IMP
Subcellular Localization
    spindle pole GO:0000922 IEA
    cytoplasm GO:0005737 IDA
    midbody GO:0030496 IEA
    microtubule plus-end GO:0035371 IDA
    nucleus GO:0005634 IDA
    condensed chromosome kinetochore GO:0000777 IEA
    mitotic spindle GO:0072686 IDA
    nucleoplasm GO:0005654 IDA
    microtubule organizing center GO:0005815 IEA
    kinetochore GO:0000776 IDA
 Experiment description of studies that identified SPAG5 in exosomes
1
Experiment ID 193
ISEV standards
EM
EV Biophysical techniques
EV Cytosolic markers
CD63|CD9
EV Membrane markers
PHB
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21595033    
Organism Homo sapiens
Experiment description Proteomic analysis of urinary exosomes from patients of early IgA nephropathy and thin basement membrane nephropathy.
Authors Moon PG, Lee JE, You S, Kim TK, Cho JH, Kim IS, Kwon TH, Kim CD, Park SH, Hwang D, Kim YL, Baek MC.
Journal name Proteomics
Publication year 2011
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
2
Experiment ID 194
ISEV standards
EV Biophysical techniques
EV Cytosolic markers
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21595033    
Organism Homo sapiens
Experiment description Proteomic analysis of urinary exosomes from patients of early IgA nephropathy and thin basement membrane nephropathy.
Authors Moon PG, Lee JE, You S, Kim TK, Cho JH, Kim IS, Kwon TH, Kim CD, Park SH, Hwang D, Kim YL, Baek MC.
Journal name Proteomics
Publication year 2011
Sample Urine
Sample name Urine - Patients of basement membrane nephropathy
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 195
ISEV standards
EV Biophysical techniques
EV Cytosolic markers
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21595033    
Organism Homo sapiens
Experiment description Proteomic analysis of urinary exosomes from patients of early IgA nephropathy and thin basement membrane nephropathy.
Authors Moon PG, Lee JE, You S, Kim TK, Cho JH, Kim IS, Kwon TH, Kim CD, Park SH, Hwang D, Kim YL, Baek MC.
Journal name Proteomics
Publication year 2011
Sample Urine
Sample name Urine - Patients of early IgA nephropathy
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for SPAG5
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 CDK1 983
Biochemical Activity Homo sapiens
2 ODF1  
Invitro Homo sapiens
Two-hybrid Homo sapiens
3 SNAPAP  
Two-hybrid Homo sapiens
View the network image/svg+xml
 Pathways in which SPAG5 is involved
No pathways found





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