Gene description for CLUH
Gene name clustered mitochondria (cluA/CLU1) homolog
Gene symbol CLUH
Other names/aliases CLU1
KIAA0664
Species Homo sapiens
 Database cross references - CLUH
ExoCarta ExoCarta_23277
Vesiclepedia VP_23277
Entrez Gene 23277
HGNC 29094
MIM 616184
UniProt O75153  
 CLUH identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Chondrocytes 35931686    
Chondrocytes 35931686    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Mesenchymal stem cells 36408942    
Prostate cancer cells 25844599    
Thymus 23844026    
 Gene ontology annotations for CLUH
Molecular Function
    mRNA binding GO:0003729 IBA
    mRNA binding GO:0003729 IDA
    protein binding GO:0005515 IPI
Biological Process
    mitochondrion organization GO:0007005 IMP
    intracellular distribution of mitochondria GO:0048312 IBA
    intracellular distribution of mitochondria GO:0048312 IMP
Subcellular Localization
    cytoplasm GO:0005737 IBA
    cytoplasm GO:0005737 IDA
    mitochondrion GO:0005739 HTP
 Experiment description of studies that identified CLUH in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 497
MISEV standards
✘ Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
6
Experiment ID 498
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
7
Experiment ID 363
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
8
Experiment ID 364
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
9
Experiment ID 365
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
10
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
11
Experiment ID 275
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|RAB5A|CD9|CD82|CD63|CD81
Enriched markers
✔
AIF
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25844599    
Organism Homo sapiens
Experiment description Molecular profiling of prostate cancer derived exosomes may reveal a predictive signature for response to docetaxel.
Authors "Kharaziha P, Chioureas D, Rutishauser D, Baltatzis G, Lennartsson L, Fonseca P, Azimi A, Hultenby K, Zubarev R, Ullen A, Yachnin J, Nilsson S, Panaretakis T."
Journal name Oncotarget
Publication year 2015
Sample Prostate cancer cells
Sample name DU145 - Docetaxel sensitive
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.12-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry/Flow cytometry/Western blotting
12
Experiment ID 217
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|CD81|CD9|CD63
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors "Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O."
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for CLUH
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 UBE2H 7328
Affinity Capture-MS Homo sapiens
2 RNGTT 8732
Affinity Capture-MS Homo sapiens
3 MCM7 4176
Co-fractionation Homo sapiens
4 PPP3CA 5530
Co-fractionation Homo sapiens
5 NOP58 51602
Co-fractionation Homo sapiens
6 WWP1 11059
Affinity Capture-MS Homo sapiens
7 ACSM5 54988
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
8 ERCC2 2068
Co-fractionation Homo sapiens
9 FOXD3  
Affinity Capture-MS Homo sapiens
10 HSP90AB2P 391634
Co-fractionation Homo sapiens
11 C9orf78 51759
Affinity Capture-MS Homo sapiens
12 PHYH 5264
Affinity Capture-MS Homo sapiens
13 ZAR1L  
Affinity Capture-MS Homo sapiens
14 CORO1B 57175
Co-fractionation Homo sapiens
15 ZNF321P  
Affinity Capture-MS Homo sapiens
16 DYNC1LI2 1783
Co-fractionation Homo sapiens
17 PMPCA 23203
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
18 ABCC12  
Affinity Capture-MS Homo sapiens
19 PSMB5 5693
Co-fractionation Homo sapiens
20 LIG1 3978
Co-fractionation Homo sapiens
21 GKAP1  
Affinity Capture-MS Homo sapiens
22 C8orf82  
Proximity Label-MS Homo sapiens
23 PSMB6 5694
Co-fractionation Homo sapiens
24 NUSAP1 51203
Co-fractionation Homo sapiens
25 DNAJC7 7266
Proximity Label-MS Homo sapiens
26 PFKFB3 5209
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
27 KNSTRN  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
28 VWA8 23078
Proximity Label-MS Homo sapiens
29 APPL1 26060
Co-fractionation Homo sapiens
30 KRT8 3856
Proximity Label-MS Homo sapiens
31 COX15 1355
Proximity Label-MS Homo sapiens
32 RPL9 6133
Co-fractionation Homo sapiens
33 MOS 4342
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
34 CLPP 8192
Proximity Label-MS Homo sapiens
35 B4GALT2  
Affinity Capture-MS Homo sapiens
36 HSP90B1 7184
Co-fractionation Homo sapiens
37 DNAJC2 27000
Proximity Label-MS Homo sapiens
38 ARHGEF1 9138
Co-fractionation Homo sapiens
39 MPPED1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
40 HSCB 150274
Proximity Label-MS Homo sapiens
41 ABCF2 10061
Co-fractionation Homo sapiens
42 TRIM16 10626
Co-fractionation Homo sapiens
43 NTRK1 4914
Affinity Capture-MS Homo sapiens
44 TRAPPC2L 51693
Affinity Capture-MS Homo sapiens
45 ACSM3  
Affinity Capture-MS Homo sapiens
46 PPA2 27068
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
47 NDUFAF1  
Affinity Capture-MS Homo sapiens
48 SIX6  
Affinity Capture-MS Homo sapiens
49 GAN 8139
Co-fractionation Homo sapiens
50 CKAP5 9793
Co-fractionation Homo sapiens
51 MRPL40 64976
Co-fractionation Homo sapiens
52 MRPL46  
Co-fractionation Homo sapiens
53 FECH 2235
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
54 F8A2  
Affinity Capture-MS Homo sapiens
55 SIRT7  
Affinity Capture-MS Homo sapiens
56 NCKAP1 10787
Co-fractionation Homo sapiens
57 OPA1 4976
Proximity Label-MS Homo sapiens
58 FAHD1 81889
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
59 MCM4 4173
Co-fractionation Homo sapiens
60 WDR37 22884
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
61 RIPK4  
Affinity Capture-MS Homo sapiens
62 SIX3  
Affinity Capture-MS Homo sapiens
63 MTUS1  
Affinity Capture-MS Homo sapiens
64 RPGRIP1L 23322
Affinity Capture-MS Homo sapiens
65 RPA3 6119
Proximity Label-MS Homo sapiens
66 DYNC1LI1 51143
Co-fractionation Homo sapiens
67 HDAC11  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
68 DLST 1743
Co-fractionation Homo sapiens
69 EEFSEC 60678
Co-fractionation Homo sapiens
70 UBL7  
Co-fractionation Homo sapiens
71 HDLBP 3069
Co-fractionation Homo sapiens
72 DIDO1  
Co-fractionation Homo sapiens
73 DNAJB13  
Proximity Label-MS Homo sapiens
74 CLPB 81570
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
75 NDUFA10 4705
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
76 MARS2 92935
Affinity Capture-MS Homo sapiens
77 HSPA9 3313
Proximity Label-MS Homo sapiens
78 NPHP4 261734
Affinity Capture-MS Homo sapiens
79 MCUR1 63933
Proximity Label-MS Homo sapiens
80 NIPSNAP3A  
Affinity Capture-MS Homo sapiens
81 ARPC3 10094
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
82 TEKT3 64518
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
83 MRPL42  
Affinity Capture-MS Homo sapiens
84 KIF2A 3796
Co-fractionation Homo sapiens
85 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
86 HMGB1 3146
Co-fractionation Homo sapiens
87 BKRF1  
Affinity Capture-MS
88 TP53 7157
Affinity Capture-MS Homo sapiens
89 THOC3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
90 RPP25L  
Affinity Capture-MS Homo sapiens
91 MCM5 4174
Co-fractionation Homo sapiens
92 KLHDC3  
Affinity Capture-MS Homo sapiens
93 PARP9 83666
Affinity Capture-MS Homo sapiens
94 YARS 8565
Co-fractionation Homo sapiens
95 COX8A  
Proximity Label-MS Homo sapiens
96 APOPT1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
97 DCAF4L2 138009
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
98 NIPSNAP3B 55335
Affinity Capture-MS Homo sapiens
99 PSMA1 5682
Co-fractionation Homo sapiens
100 MAGIX 79917
Affinity Capture-MS Homo sapiens
101 SPAG5 10615
Affinity Capture-MS Homo sapiens
102 TEFM  
Affinity Capture-MS Homo sapiens
103 PRKCA 5578
Co-fractionation Homo sapiens
104 PINK1  
Affinity Capture-MS Homo sapiens
105 WDR90  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
106 PER3  
Affinity Capture-MS Homo sapiens
107 CEBPB  
Co-fractionation Homo sapiens
108 PRPF4 9128
Co-fractionation Homo sapiens
109 HSPA4L 22824
Co-fractionation Homo sapiens
110 CYFIP1 23191
Co-fractionation Homo sapiens
111 DCAF4  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
112 UBA6 55236
Co-fractionation Homo sapiens
113 MCEE 84693
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which CLUH is involved
No pathways found





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