Gene description for PARP9
Gene name poly (ADP-ribose) polymerase family, member 9
Gene symbol PARP9
Other names/aliases ARTD9
BAL
BAL1
MGC:7868
Species Homo sapiens
 Database cross references - PARP9
ExoCarta ExoCarta_83666
Vesiclepedia VP_83666
Entrez Gene 83666
HGNC 24118
MIM 612065
UniProt Q8IXQ6  
 PARP9 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Mesenchymal stem cells 36408942    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Thymus 23844026    
 Gene ontology annotations for PARP9
Molecular Function
    transcription corepressor activity GO:0003714 IBA
    transcription corepressor activity GO:0003714 IMP
    NAD+-protein poly-ADP-ribosyltransferase activity GO:0003950 IDA
    NAD+-protein poly-ADP-ribosyltransferase activity GO:0003950 IMP
    NAD+-protein poly-ADP-ribosyltransferase activity GO:0003950 TAS
    enzyme inhibitor activity GO:0004857 IDA
    protein binding GO:0005515 IPI
    nucleotidyltransferase activity GO:0016779 IEA
    enzyme binding GO:0019899 IPI
    histone binding GO:0042393 IPI
    ubiquitin-like protein ligase binding GO:0044389 IBA
    ubiquitin-like protein ligase binding GO:0044389 IPI
    NAD+ binding GO:0070403 IMP
    ADP-D-ribose binding GO:0072570 IMP
    STAT family protein binding GO:0097677 IPI
    NAD+-protein-C-terminal glycine ADP-ribosyltransferase activity GO:0140802 IDA
    NAD+-protein ADP-ribosyltransferase activity GO:1990404 IBA
Biological Process
    DNA damage checkpoint signaling GO:0000077 IMP
    negative regulation of transcription by RNA polymerase II GO:0000122 IMP
    positive regulation of defense response to virus by host GO:0002230 IGI
    double-strand break repair GO:0006302 IMP
    post-transcriptional regulation of gene expression GO:0010608 IMP
    negative regulation of gene expression GO:0010629 IBA
    negative regulation of gene expression GO:0010629 IMP
    cell migration GO:0016477 TAS
    viral protein processing GO:0019082 TAS
    NAD biosynthesis via nicotinamide riboside salvage pathway GO:0034356 TAS
    positive regulation of chromatin binding GO:0035563 IGI
    positive regulation of tyrosine phosphorylation of STAT protein GO:0042531 IMP
    negative regulation of catalytic activity GO:0043086 IDA
    innate immune response GO:0045087 IEA
    positive regulation of DNA-templated transcription GO:0045893 IGI
    defense response to virus GO:0051607 IEA
    regulation of response to type II interferon GO:0060330 IDA
    positive regulation of type II interferon-mediated signaling pathway GO:0060335 IBA
    positive regulation of type II interferon-mediated signaling pathway GO:0060335 IMP
    protein poly-ADP-ribosylation GO:0070212 IMP
    positive regulation of protein localization to nucleus GO:1900182 IGI
Subcellular Localization
    nucleus GO:0005634 IC
    nucleus GO:0005634 IDA
    nucleus GO:0005634 IDA
    nucleus GO:0005634 TAS
    nucleoplasm GO:0005654 IDA
    cytoplasm GO:0005737 IBA
    cytoplasm GO:0005737 IDA
    mitochondrion GO:0005739 IDA
    cytosol GO:0005829 IDA
    cytosol GO:0005829 TAS
    membrane GO:0016020 HDA
    protein-containing complex GO:0032991 IDA
    site of DNA damage GO:0090734 IDA
 Experiment description of studies that identified PARP9 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
6
Experiment ID 211
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
cytochrome c|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
7
Experiment ID 212
MISEV standards
✔
CEM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
Cytochrome C|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
8
Experiment ID 217
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|CD81|CD9|CD63
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors "Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O."
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for PARP9
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 NAA25 80018
Co-fractionation Homo sapiens
2 RPA2 6118
Affinity Capture-MS Homo sapiens
3 RBM43 375287
Affinity Capture-MS Homo sapiens
4 RPA1 6117
Affinity Capture-MS Homo sapiens
5 PML 5371
Affinity Capture-MS Homo sapiens
6 HIST1H2BJ 8970
Affinity Capture-Western Homo sapiens
7 ANXA1 301
Protein-peptide Homo sapiens
8 CUL3 8452
Affinity Capture-MS Homo sapiens
9 MEX3A  
Affinity Capture-RNA Homo sapiens
10 STAT1 6772
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
11 PARP1 142
Co-localization Homo sapiens
Affinity Capture-Western Homo sapiens
12 DTX3L 151636
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
13 RPA3 6119
Affinity Capture-MS Homo sapiens
14 PARP14 54625
Affinity Capture-Western Homo sapiens
15 CLUH 23277
Affinity Capture-MS Homo sapiens
16 RNF166  
Affinity Capture-MS Homo sapiens
17 SORT1 6272
Affinity Capture-MS Homo sapiens
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