Gene description for DTX3L
Gene name deltex 3 like, E3 ubiquitin ligase
Gene symbol DTX3L
Other names/aliases BBAP
Species Homo sapiens
 Database cross references - DTX3L
ExoCarta ExoCarta_151636
Vesiclepedia VP_151636
Entrez Gene 151636
HGNC 30323
MIM 613143
UniProt Q8TDB6  
 DTX3L identified in exosomes derived from the following tissue/cell type
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Endothelial cells 26027894    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
 Gene ontology annotations for DTX3L
Molecular Function
    ubiquitin-protein transferase activity GO:0004842 IDA
    ubiquitin-protein transferase activity GO:0004842 IMP
    enzyme inhibitor activity GO:0004857 IMP
    protein binding GO:0005515 IPI
    enzyme binding GO:0019899 IPI
    histone binding GO:0042393 IDA
    histone binding GO:0042393 IPI
    ubiquitin-like protein ligase binding GO:0044389 IPI
    metal ion binding GO:0046872 IEA
    ubiquitin protein ligase activity GO:0061630 IBA
    STAT family protein binding GO:0097677 IPI
    protein ADP-ribosyltransferase-substrate adaptor activity GO:0140768 IDA
    histone ubiquitin ligase activity GO:0140852 IMP
    histone H4K91 ubiquitin ligase activity GO:0141000 IDA
Biological Process
    DNA damage checkpoint signaling GO:0000077 IMP
    positive regulation of defense response to virus by host GO:0002230 IGI
    double-strand break repair GO:0006302 IMP
    ubiquitin-dependent protein catabolic process GO:0006511 IMP
    Notch signaling pathway GO:0007219 IBA
    Notch signaling pathway GO:0007219 IEA
    endosome to lysosome transport GO:0008333 IMP
    protein transport GO:0015031 IEA
    protein ubiquitination GO:0016567 IBA
    positive regulation of protein binding GO:0032092 IMP
    positive regulation of chromatin binding GO:0035563 IGI
    innate immune response GO:0045087 IEA
    positive regulation of DNA-templated transcription GO:0045893 IGI
    negative regulation of ubiquitin-protein transferase activity GO:0051444 IMP
    defense response to virus GO:0051607 IEA
    protein autoubiquitination GO:0051865 IMP
    protein K48-linked ubiquitination GO:0070936 IDA
    DNA repair-dependent chromatin remodeling GO:0140861 IMP
    positive regulation of protein localization to nucleus GO:1900182 IGI
    positive regulation of protein localization to early endosome GO:1902966 IMP
    positive regulation of receptor catabolic process GO:2000646 IMP
Subcellular Localization
    nucleus GO:0005634 IDA
    nucleus GO:0005634 IDA
    nucleoplasm GO:0005654 IBA
    nucleoplasm GO:0005654 IDA
    cytoplasm GO:0005737 IDA
    lysosome GO:0005764 IDA
    lysosomal membrane GO:0005765 IEA
    early endosome GO:0005769 IDA
    cytosol GO:0005829 IDA
    cytosol GO:0005829 TAS
    early endosome membrane GO:0031901 IEA
    protein-containing complex GO:0032991 IDA
 Experiment description of studies that identified DTX3L in exosomes
1
Experiment ID 207
MISEV standards
EM
Biophysical techniques
TSG101|HSP70|FLOT1
Enriched markers
VDAC
Negative markers
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DKO-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
2
Experiment ID 208
MISEV standards
EM
Biophysical techniques
TSG101|HSP70|FLOT1
Enriched markers
VDAC
Negative markers
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name Dks-8
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 209
MISEV standards
Biophysical techniques
TSG101|HSP70|FLOT1
Enriched markers
VDAC
Negative markers
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DLD-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 226
MISEV standards
EM
Biophysical techniques
GAPDH|CD9|FLOT1
Enriched markers
LMNA|H2AFX|ATP5A1|TOMM20
Negative markers
Particle analysis
Identified molecule mRNA
Identification method Small RNA sequencing (Illumina HiSeq 2000 (Solexa)
PubMed ID 26027894    
Organism Homo sapiens
Experiment description "Quantitative and qualitative analysis of small RNAs in human endothelial cells and exosomes provides insights into localized RNA processing, degradation and sorting"
Authors "Bas W. M. van Balkom, Almut S. Eisele, D. Michiel Pegtel, Sander Bervoets, Marianne C. Verhaar"
Journal name Journal of Extracellular Vesicles
Publication year 2015
Sample Endothelial cells
Sample name HMEC-1
Isolation/purification methods Differential ultracentrifugation
Sucrose density gradient
Flotation density 1.10 g/mL
Molecules identified in the study miRNA
Protein
snoRNA
lncRNA
yRNA
snRNA
mRNA
ncRNA
mtRNA
vtRNA
scaRNA
lincRNA
Methods used in the study Small RNA sequencing (Illumina HiSeq 2000 (Solexa)
Western blotting
5
Experiment ID 211
MISEV standards
EM
Biophysical techniques
TSG101|Alix|EpCAM|TFRC
Enriched markers
cytochrome c|GOLGA2
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
6
Experiment ID 212
MISEV standards
CEM
Biophysical techniques
TSG101|Alix|EpCAM|TFRC
Enriched markers
Cytochrome C|GOLGA2
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for DTX3L
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 UBE2H 7328
Two-hybrid Homo sapiens
2 PHF7  
Two-hybrid Homo sapiens
3 UBE2W  
Two-hybrid Homo sapiens
4 PARP14 54625
Affinity Capture-Western Homo sapiens
5 PARP1 142
Co-localization Homo sapiens
6 DTX3L 151636
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
Co-purification Homo sapiens
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
Co-purification Homo sapiens
Biochemical Activity Homo sapiens
7 APPL1 26060
Affinity Capture-MS Homo sapiens
8 HIST1H4A 8359
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
9 RPA2 6118
Affinity Capture-MS Homo sapiens
10 UBE2E3 10477
Two-hybrid Homo sapiens
11 RBM43 375287
Affinity Capture-MS Homo sapiens
12 RPA1 6117
Affinity Capture-MS Homo sapiens
13 SPG21 51324
Two-hybrid Homo sapiens
14 HIST1H2BJ 8970
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
15 CUL3 8452
Affinity Capture-MS Homo sapiens
16 PARP9 83666
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
17 UBE2E1  
Two-hybrid Homo sapiens
18 ELAVL1 1994
Affinity Capture-RNA Homo sapiens
19 TRIM9  
Two-hybrid Homo sapiens
20 ANTXR1 84168
Two-hybrid Homo sapiens
21 UBE2D4  
Two-hybrid Homo sapiens
22 DTX1  
Affinity Capture-Western Homo sapiens
23 UBE2R2 54926
Two-hybrid Homo sapiens
24 IFNGR1 3459
Affinity Capture-Western Homo sapiens
25 UBE2D2 7322
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
26 UBE2I 7329
Two-hybrid Homo sapiens
27 STAT1 6772
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
28 RPA3 6119
Affinity Capture-MS Homo sapiens
29 JAK1 3716
Affinity Capture-Western Homo sapiens
30 UBE2E2  
Two-hybrid Homo sapiens
31 UBE2K 3093
Two-hybrid Homo sapiens
32 RNF111 54778
Two-hybrid Homo sapiens
33 HGS 9146
Affinity Capture-Western Homo sapiens
34 H2AFV 94239
Biochemical Activity Homo sapiens
35 PTPN23 25930
Proximity Label-MS Homo sapiens
36 LCN1 3933
Affinity Capture-MS Homo sapiens
37 PML 5371
Affinity Capture-MS Homo sapiens
38 UBE2D3 7323
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
39 JAK2 3717
Affinity Capture-Western Homo sapiens
40 ITCH 83737
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
41 STAM 8027
Affinity Capture-Western Homo sapiens
42 HIST1H2AB 8335
Biochemical Activity Homo sapiens
43 NCAPG 64151
Affinity Capture-MS Homo sapiens
44 UBE2D1 7321
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
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