Gene ontology annotations for DTX3L
Experiment description of studies that identified DTX3L in exosomes
1
Experiment ID
207
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP70|FLOT1
Enriched markers
✔
VDAC
Negative markers
✔
NTA
Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
23161513
Organism
Homo sapiens
Experiment description
Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors
"Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name
Mol Cell Proteomics
Publication year
2012
Sample
Colorectal cancer cells
Sample name
DKO-1
Isolation/purification methods
Differential centrifugation Filtration
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry
2
Experiment ID
208
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP70|FLOT1
Enriched markers
✔
VDAC
Negative markers
✔
NTA
Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
23161513
Organism
Homo sapiens
Experiment description
Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors
"Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name
Mol Cell Proteomics
Publication year
2012
Sample
Colorectal cancer cells
Sample name
Dks-8
Isolation/purification methods
Differential centrifugation Filtration
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry
3
Experiment ID
209
MISEV standards
✘
Biophysical techniques
✔
TSG101|HSP70|FLOT1
Enriched markers
✔
VDAC
Negative markers
✔
NTA
Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
23161513
Organism
Homo sapiens
Experiment description
Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors
"Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name
Mol Cell Proteomics
Publication year
2012
Sample
Colorectal cancer cells
Sample name
DLD-1
Isolation/purification methods
Differential centrifugation Filtration
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry
4
Experiment ID
226
MISEV standards
✔
EM
Biophysical techniques
✔
GAPDH|CD9|FLOT1
Enriched markers
✔
LMNA|H2AFX|ATP5A1|TOMM20
Negative markers
✘
Particle analysis
Identified molecule
mRNA
Identification method
Small RNA sequencing (Illumina HiSeq 2000 (Solexa)
PubMed ID
26027894
Organism
Homo sapiens
Experiment description
"Quantitative and qualitative analysis of small RNAs in human endothelial cells and exosomes provides insights into localized RNA processing, degradation and sorting"
Authors
"Bas W. M. van Balkom, Almut S. Eisele, D. Michiel Pegtel, Sander Bervoets, Marianne C. Verhaar"
Journal name
Journal of Extracellular Vesicles
Publication year
2015
Sample
Endothelial cells
Sample name
HMEC-1
Isolation/purification methods
Differential ultracentrifugation Sucrose density gradient
Flotation density
1.10 g/mL
Molecules identified in the study
miRNA Protein snoRNA lncRNA yRNA snRNA mRNA ncRNA mtRNA vtRNA scaRNA lincRNA
Methods used in the study
Small RNA sequencing (Illumina HiSeq 2000 (Solexa) Western blotting
5
Experiment ID
211
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
cytochrome c|GOLGA2
Negative markers
✘
Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
23333927
Organism
Homo sapiens
Experiment description
Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors
"Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name
J Proteomics
Publication year
2013
Sample
Ovarian cancer cells
Sample name
IGROV1
Isolation/purification methods
Differential centrifugation Ultracentrifugation Sucrose density gradient
Flotation density
1.09-1.15 g/mL
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry
6
Experiment ID
212
MISEV standards
✔
CEM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
Cytochrome C|GOLGA2
Negative markers
✘
Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
23333927
Organism
Homo sapiens
Experiment description
Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors
"Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name
J Proteomics
Publication year
2013
Sample
Ovarian cancer cells
Sample name
OVCAR-3
Isolation/purification methods
Differential centrifugation Ultracentrifugation Sucrose density gradient
Flotation density
1.09-1.15 g/mL
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry
Protein-protein interactions for DTX3L
Protein Interactor
ExoCarta ID
Identification method
PubMed
Species
1
UBE2H
7328
Two-hybrid
Homo sapiens
2
PHF7
Two-hybrid
Homo sapiens
3
UBE2W
Two-hybrid
Homo sapiens
4
PARP14
54625
Affinity Capture-Western
Homo sapiens
5
PARP1
142
Co-localization
Homo sapiens
6
DTX3L
151636
Affinity Capture-Western
Homo sapiens
Affinity Capture-Western
Homo sapiens
Biochemical Activity
Homo sapiens
Co-purification
Homo sapiens
Biochemical Activity
Homo sapiens
Affinity Capture-Western
Homo sapiens
Affinity Capture-Western
Homo sapiens
Biochemical Activity
Homo sapiens
Co-purification
Homo sapiens
Biochemical Activity
Homo sapiens
7
APPL1
26060
Affinity Capture-MS
Homo sapiens
8
HIST1H4A
8359
Biochemical Activity
Homo sapiens
Affinity Capture-Western
Homo sapiens
9
RPA2
6118
Affinity Capture-MS
Homo sapiens
10
UBE2E3
10477
Two-hybrid
Homo sapiens
11
RBM43
375287
Affinity Capture-MS
Homo sapiens
12
RPA1
6117
Affinity Capture-MS
Homo sapiens
13
SPG21
51324
Two-hybrid
Homo sapiens
14
HIST1H2BJ
8970
Affinity Capture-Western
Homo sapiens
Biochemical Activity
Homo sapiens
Affinity Capture-Western
Homo sapiens
15
CUL3
8452
Affinity Capture-MS
Homo sapiens
16
PARP9
83666
Affinity Capture-Western
Homo sapiens
Affinity Capture-Western
Homo sapiens
Affinity Capture-Western
Homo sapiens
Affinity Capture-Western
Homo sapiens
Affinity Capture-Western
Homo sapiens
Affinity Capture-MS
Homo sapiens
Reconstituted Complex
Homo sapiens
Affinity Capture-MS
Homo sapiens
Reconstituted Complex
Homo sapiens
Affinity Capture-MS
Homo sapiens
17
UBE2E1
Two-hybrid
Homo sapiens
18
ELAVL1
1994
Affinity Capture-RNA
Homo sapiens
19
TRIM9
Two-hybrid
Homo sapiens
20
ANTXR1
84168
Two-hybrid
Homo sapiens
21
UBE2D4
Two-hybrid
Homo sapiens
22
DTX1
Affinity Capture-Western
Homo sapiens
23
UBE2R2
54926
Two-hybrid
Homo sapiens
24
IFNGR1
3459
Affinity Capture-Western
Homo sapiens
25
UBE2D2
7322
Two-hybrid
Homo sapiens
Affinity Capture-Western
Homo sapiens
Reconstituted Complex
Homo sapiens
26
UBE2I
7329
Two-hybrid
Homo sapiens
27
STAT1
6772
Affinity Capture-Western
Homo sapiens
Affinity Capture-Western
Homo sapiens
Affinity Capture-Western
Homo sapiens
28
RPA3
6119
Affinity Capture-MS
Homo sapiens
29
JAK1
3716
Affinity Capture-Western
Homo sapiens
30
UBE2E2
Two-hybrid
Homo sapiens
31
UBE2K
3093
Two-hybrid
Homo sapiens
32
RNF111
54778
Two-hybrid
Homo sapiens
33
HGS
9146
Affinity Capture-Western
Homo sapiens
34
H2AFV
94239
Biochemical Activity
Homo sapiens
35
PTPN23
25930
Proximity Label-MS
Homo sapiens
36
LCN1
3933
Affinity Capture-MS
Homo sapiens
37
PML
5371
Affinity Capture-MS
Homo sapiens
38
UBE2D3
7323
Two-hybrid
Homo sapiens
Affinity Capture-Western
Homo sapiens
39
JAK2
3717
Affinity Capture-Western
Homo sapiens
40
ITCH
83737
Reconstituted Complex
Homo sapiens
Affinity Capture-Western
Homo sapiens
Reconstituted Complex
Homo sapiens
41
STAM
8027
Affinity Capture-Western
Homo sapiens
42
HIST1H2AB
8335
Biochemical Activity
Homo sapiens
43
NCAPG
64151
Affinity Capture-MS
Homo sapiens
44
UBE2D1
7321
Two-hybrid
Homo sapiens
Affinity Capture-Western
Homo sapiens
Reconstituted Complex
Homo sapiens
Reconstituted Complex
Homo sapiens
View the network
image/svg+xml
Pathways in which DTX3L is involved