Gene description for SPG21
Gene name spastic paraplegia 21 (autosomal recessive, Mast syndrome)
Gene symbol SPG21
Other names/aliases ACP33
BM-019
GL010
MAST
Species Homo sapiens
 Database cross references - SPG21
ExoCarta ExoCarta_51324
Vesiclepedia VP_51324
Entrez Gene 51324
HGNC 20373
MIM 608181
UniProt Q9NZD8  
 SPG21 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Hepatocellular carcinoma cells 26054723    
Thymus 23844026    
 Gene ontology annotations for SPG21
Molecular Function
    protein binding GO:0005515 IPI
    CD4 receptor binding GO:0042609 IBA
    CD4 receptor binding GO:0042609 IPI
Biological Process
    epidermal growth factor receptor signaling pathway GO:0007173 IEA
    locomotory behavior GO:0007626 IEA
    gene expression GO:0010467 IEA
    neuron maturation GO:0042551 IEA
    collateral sprouting GO:0048668 IEA
    antigen receptor-mediated signaling pathway GO:0050851 IC
    neuromuscular process GO:0050905 IEA
    limb development GO:0060173 IEA
    response to epidermal growth factor GO:0070849 IEA
Subcellular Localization
    Golgi apparatus GO:0005794 IEA
    cytosol GO:0005829 IBA
    cytosol GO:0005829 IDA
    endosome membrane GO:0010008 IEA
    trans-Golgi network transport vesicle GO:0030140 IBA
    trans-Golgi network transport vesicle GO:0030140 IDA
    intracellular membrane-bounded organelle GO:0043231 IDA
 Experiment description of studies that identified SPG21 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 235
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
6
Experiment ID 217
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|CD81|CD9|CD63
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors "Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O."
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for SPG21
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 XXYLT1 152002
Affinity Capture-MS Homo sapiens
2 VPS35 55737
Affinity Capture-MS Homo sapiens
3 PLS1 5357
Affinity Capture-MS Homo sapiens
4 TRIM54  
Two-hybrid Homo sapiens
5 CMTM5  
Two-hybrid Homo sapiens
6 MICAL1 64780
Affinity Capture-MS Homo sapiens
7 TRAF2 7186
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
8 SPRED2 200734
Two-hybrid Homo sapiens
9 EFHC2  
Two-hybrid Homo sapiens
10 FAM114A1 92689
Two-hybrid Homo sapiens
11 LAMTOR1 55004
Proximity Label-MS Homo sapiens
12 KHK 3795
Affinity Capture-MS Homo sapiens
13 CTPS2 56474
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
14 IKZF3  
Two-hybrid Homo sapiens
15 APOO 79135
Affinity Capture-MS Homo sapiens
16 CD4 920
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
17 CRYAA 1409
Two-hybrid Homo sapiens
18 INCA1  
Two-hybrid Homo sapiens
19 GAPDHS 26330
Affinity Capture-MS Homo sapiens
20 VAMP7 6845
Affinity Capture-MS Homo sapiens
21 ARL8A 127829
Affinity Capture-MS Homo sapiens
22 CCDC33 80125
Two-hybrid Homo sapiens
23 YLPM1 56252
Co-fractionation Homo sapiens
24 HMGCS1 3157
Co-fractionation Homo sapiens
25 ARL6IP1 23204
Two-hybrid Homo sapiens
26 RTN4 57142
Two-hybrid Homo sapiens
27 TRIM9  
Two-hybrid Homo sapiens
28 MTMR9 66036
Two-hybrid Homo sapiens
29 TFG 10342
Two-hybrid Homo sapiens
30 VPS29 51699
Affinity Capture-MS Homo sapiens
31 FAM50A 9130
Affinity Capture-MS Homo sapiens
32 PCNA 5111
Co-fractionation Homo sapiens
33 GSK3A 2931
Two-hybrid Homo sapiens
34 DHX40  
Co-fractionation Homo sapiens
35 AGTRAP 57085
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
36 ARL8B 55207
Affinity Capture-MS Homo sapiens
37 KRT15 3866
Two-hybrid Homo sapiens
38 TCF4  
Two-hybrid Homo sapiens
39 MOV10 4343
Affinity Capture-RNA Homo sapiens
40 RABAC1 10567
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
41 TRIM23 373
Two-hybrid Homo sapiens
42 TCF12  
Two-hybrid Homo sapiens
43 RAB7A 7879
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
44 RAB9A 9367
Proximity Label-MS Homo sapiens
45 AKIRIN2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
46 REEP6  
Two-hybrid Homo sapiens
47 DTX2 113878
Two-hybrid Homo sapiens
Proximity Label-MS Homo sapiens
Two-hybrid Homo sapiens
48 HSPA4 3308
Co-fractionation Homo sapiens
49 ATPAF2 91647
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
50 SERBP1 26135
Affinity Capture-MS Homo sapiens
51 S100B 6285
Two-hybrid Homo sapiens
52 GGA2 23062
Two-hybrid Homo sapiens
53 CUTC  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
54 SRC 6714
Affinity Capture-MS Homo sapiens
55 GNAZ 2781
Affinity Capture-MS Homo sapiens
56 DTX3L 151636
Two-hybrid Homo sapiens
57 PRPS1 5631
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
58 FAM213A 84293
Affinity Capture-MS Homo sapiens
59 AGTR1  
Two-hybrid Homo sapiens
60 CCDC102B  
Two-hybrid Homo sapiens
61 MID2 11043
Two-hybrid Homo sapiens
62 ZNF263  
Two-hybrid Homo sapiens
63 RRAGB 10325
Affinity Capture-MS Homo sapiens
64 TRAF1 7185
Two-hybrid Homo sapiens
View the network image/svg+xml
 Pathways in which SPG21 is involved
No pathways found





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