Gene description for FAM50A
Gene name family with sequence similarity 50, member A
Gene symbol FAM50A
Other names/aliases 9F
DXS9928E
HXC-26
HXC26
XAP5
Species Homo sapiens
 Database cross references - FAM50A
ExoCarta ExoCarta_9130
Vesiclepedia VP_9130
Entrez Gene 9130
HGNC 18786
MIM 300453
UniProt Q14320  
 FAM50A identified in exosomes derived from the following tissue/cell type
Breast cancer cells 34108659    
Breast cancer cells 34108659    
Embryonic kidney cells 34108659    
Embryonic kidney cells 34108659    
Pancreatic cancer cells 34108659    
Pancreatic cancer cells 34108659    
 Gene ontology annotations for FAM50A
Molecular Function
    RNA binding GO:0003723 HDA
    protein binding GO:0005515 IPI
Biological Process
    chromatin organization GO:0006325 IBA
    mRNA processing GO:0006397 IEA
    spermatogenesis GO:0007283 NAS
    RNA splicing GO:0008380 IEA
    regulation of RNA splicing GO:0043484 IMP
Subcellular Localization
    nucleus GO:0005634 IBA
    nucleus GO:0005634 IDA
    nucleus GO:0005634 NAS
    nucleoplasm GO:0005654 IDA
    nucleoplasm GO:0005654 TAS
 Experiment description of studies that identified FAM50A in exosomes
1
Experiment ID 426
MISEV standards
Biophysical techniques
SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
CANX
Negative markers
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Breast cancer cells
Sample name MDA-MB-231 - Exo-rich fractions 7-10 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 427
MISEV standards
Biophysical techniques
CD81|SDCBP|FLOT1|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
CANX
Negative markers
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Breast cancer cells
Sample name MDA-MB-231 - Exo-rich fractions 1-6 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 419
MISEV standards
Biophysical techniques
SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
CANX
Negative markers
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T - Exo-rich fractions 7-10 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
4
Experiment ID 419
MISEV standards
Biophysical techniques
SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
CANX
Negative markers
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T - Exo-rich fractions 7-10 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
5
Experiment ID 420
MISEV standards
Biophysical techniques
CD81|SDCBP|FLOT1|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
CANX
Negative markers
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T - Exo-rich fractions 1-6 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
6
Experiment ID 420
MISEV standards
Biophysical techniques
CD81|SDCBP|FLOT1|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
CANX
Negative markers
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T - Exo-rich fractions 1-6 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
7
Experiment ID 434
MISEV standards
Biophysical techniques
SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
CANX
Negative markers
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Pancreatic cancer cells
Sample name PANC-1 - Exo-rich fractions 7-10 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
8
Experiment ID 435
MISEV standards
Biophysical techniques
CD81|SDCBP|FLOT1|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
CANX
Negative markers
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Pancreatic cancer cells
Sample name PANC-1 - Exo-rich fractions 1-6 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for FAM50A
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 CD3EAP  
Proximity Label-MS Homo sapiens
2 SNRPA 6626
Cross-Linking-MS (XL-MS) Homo sapiens
3 SPATS2  
Affinity Capture-MS Homo sapiens
4 RNF40 9810
Affinity Capture-MS Homo sapiens
5 ZBTB2 57621
Affinity Capture-MS Homo sapiens
6 EAPP  
Affinity Capture-MS Homo sapiens
7 PARP1 142
Proximity Label-MS Homo sapiens
8 RPS3 6188
Cross-Linking-MS (XL-MS) Homo sapiens
9 CLIP1 6249
Affinity Capture-MS Homo sapiens
10 RPL19 6143
Cross-Linking-MS (XL-MS) Homo sapiens
11 C10orf107  
Affinity Capture-MS Homo sapiens
12 FBXL6  
Proximity Label-MS Homo sapiens
13 GPI 2821
Cross-Linking-MS (XL-MS) Homo sapiens
14 C9orf78 51759
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
15 LMNB1 4001
Proximity Label-MS Homo sapiens
16 ARPC2 10109
Affinity Capture-MS Homo sapiens
17 USP22 23326
Affinity Capture-MS Homo sapiens
18 FAM50B  
Negative Genetic Homo sapiens
Affinity Capture-MS Homo sapiens
Cross-Linking-MS (XL-MS) Homo sapiens
19 RPS20 6224
Cross-Linking-MS (XL-MS) Homo sapiens
20 CD2BP2 10421
Affinity Capture-MS Homo sapiens
21 STAM2 10254
Co-fractionation Homo sapiens
22 HIST1H2BG 8339
Proximity Label-MS Homo sapiens
23 RPL21 6144
Cross-Linking-MS (XL-MS) Homo sapiens
Cross-Linking-MS (XL-MS) Homo sapiens
24 SPG21 51324
Affinity Capture-MS Homo sapiens
25 DHX8 1659
Proximity Label-MS Homo sapiens
26 ATP5F1 515
Affinity Capture-MS Homo sapiens
27 ERCC3  
Co-fractionation Homo sapiens
28 PPIA 5478
Cross-Linking-MS (XL-MS) Homo sapiens
29 CAPZB 832
Affinity Capture-MS Homo sapiens
30 DDX58 23586
Affinity Capture-RNA Homo sapiens
31 NCDN 23154
Affinity Capture-MS Homo sapiens
32 APP 351
Reconstituted Complex Homo sapiens
33 ZNF696  
Affinity Capture-MS Homo sapiens
34 C10orf62  
Affinity Capture-MS Homo sapiens
35 NXF1 10482
Affinity Capture-RNA Homo sapiens
36 APEX1 328
Proximity Label-MS Homo sapiens
37 EFTUD2 9343
Affinity Capture-MS Homo sapiens
38 CBX3 11335
Proximity Label-MS Homo sapiens
39 MEIS2  
Affinity Capture-MS Homo sapiens
40 DDX23 9416
Proximity Label-MS Homo sapiens
41 MBNL1 4154
Affinity Capture-MS Homo sapiens
42 C12orf49  
Negative Genetic Homo sapiens
43 MOV10 4343
Affinity Capture-RNA Homo sapiens
44 PRPF19 27339
Affinity Capture-MS Homo sapiens
45 RPA3 6119
Proximity Label-MS Homo sapiens
46 RPL31 6160
Proximity Label-MS Homo sapiens
47 Snw1  
Affinity Capture-MS Mus musculus
48 PRPF8 10594
Affinity Capture-MS Homo sapiens
49 IFNA1  
Affinity Capture-MS Homo sapiens
50 TCEB2 6923
Co-fractionation Homo sapiens
51 KRAS 3845
Synthetic Lethality Homo sapiens
52 SNRNP200 23020
Affinity Capture-MS Homo sapiens
53 PKM 5315
Cross-Linking-MS (XL-MS) Homo sapiens
54 MRPL38  
Affinity Capture-MS Homo sapiens
55 METTL16 79066
Co-fractionation Homo sapiens
56 LMNA 4000
Proximity Label-MS Homo sapiens
57 GSPT1 2935
Affinity Capture-MS Homo sapiens
58 ZNF14  
Affinity Capture-MS Homo sapiens
59 EIF5 1983
Affinity Capture-MS Homo sapiens
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