Gene description for TCEB2
Gene name transcription elongation factor B (SIII), polypeptide 2 (18kDa, elongin B)
Gene symbol TCEB2
Other names/aliases ELOB
SIII
Species Homo sapiens
 Database cross references - TCEB2
ExoCarta ExoCarta_6923
Entrez Gene 6923
HGNC 11619
MIM 600787
UniProt Q15370  
 TCEB2 identified in exosomes derived from the following tissue/cell type
Neuroblastoma cells 25944692    
Squamous carcinoma cells 20124223    
 Gene ontology annotations for TCEB2
Molecular Function
    protein binding GO:0005515 IPI
    ubiquitin protein ligase binding GO:0031625 IDA
Biological Process
    gene expression GO:0010467 TAS
    protein ubiquitination GO:0016567 IEA
    transcription elongation from RNA polymerase II promoter GO:0006368 TAS
    transcription from RNA polymerase II promoter GO:0006366 TAS
    regulation of transcription from RNA polymerase II promoter in response to hypoxia GO:0061418 TAS
    viral process GO:0016032 TAS
    cellular response to hypoxia GO:0071456 TAS
    positive regulation of viral transcription GO:0050434 TAS
    protein complex assembly GO:0006461 TAS
Subcellular Localization
    extracellular exosome GO:0070062 IDA
    cytosol GO:0005829 TAS
    VCB complex GO:0030891 IBA
    elongin complex GO:0070449 IBA
    Cul5-RING ubiquitin ligase complex GO:0031466 IDA
    Cul2-RING ubiquitin ligase complex GO:0031462 IDA
    nucleoplasm GO:0005654 TAS
 Experiment description of studies that identified TCEB2 in exosomes
1
Experiment ID 224
ISEV standards
EM|AFM
EV Biophysical techniques
Alix|TSG101
EV Cytosolic markers
CD63|CD81
EV Membrane markers
GOLGA2
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25944692    
Organism Homo sapiens
Experiment description Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes
Authors Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S
Journal name Oncotarget
Publication year 2015
Sample Neuroblastoma cells
Sample name SH-SY5Y
Isolation/purification methods Differential centrifugation
Ultracentrifugation
OptiPrep density gradient
Flotation density 1.10 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
2
Experiment ID 191
ISEV standards
EV Biophysical techniques
Alix
EV Cytosolic markers
CD81|CD9
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20124223    
Organism Homo sapiens
Experiment description Hypoxic tumor cell modulates its microenvironment to enhance angiogenic and metastatic potential by secretion of proteins and exosomes.
Authors Park JE, Tan HS, Datta A, Lai RC, Zhang H, Meng W, Lim SK, Sze SK.
Journal name Mol Cell Proteomics
Publication year 2010
Sample Squamous carcinoma cells
Sample name Squamous carcinoma cell (A431)
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for TCEB2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 ASB8  
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
2 SOCS6  
Invitro Homo sapiens
3 TCEB3 6924
Invitro Homo sapiens
4 LRRC41  
Invitro Homo sapiens
Affinity Capture-MS Homo sapiens
5 TCEB1 6921
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
Invitro Homo sapiens
6 VHL  
Invivo Homo sapiens
Invivo Homo sapiens
Invitro Homo sapiens
Invitro Homo sapiens
Invivo Homo sapiens
Invitro Homo sapiens
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
7 USP33  
Invivo Homo sapiens
Affinity Capture-MS Homo sapiens
View the network image/svg+xml



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