Gene description for Pcna
Gene name proliferating cell nuclear antigen
Gene symbol Pcna
Other names/aliases -
Species Mus musculus
 Database cross references - Pcna
ExoCarta ExoCarta_18538
Entrez Gene 18538
UniProt P17918  
 Pcna identified in exosomes derived from the following tissue/cell type
Brain cancer cells 19109410    
Fibroblasts 23260141    
Pancreatic cells 19351151    
 Gene ontology annotations for Pcna
Molecular Function
    DNA polymerase binding GO:0070182 ISO
    MutLalpha complex binding GO:0032405 ISO
    DNA binding GO:0003677 IEA
    histone acetyltransferase binding GO:0035035 ISO
    chromatin binding GO:0003682 ISO
    damaged DNA binding GO:0003684 ISO
    DNA polymerase processivity factor activity GO:0030337 IBA
    dinucleotide insertion or deletion binding GO:0032139 ISO
    identical protein binding GO:0042802 ISO
    purine-specific mismatch base pair DNA N-glycosylase activity GO:0000701 ISO
    estrogen receptor binding GO:0030331 ISO
    receptor tyrosine kinase binding GO:0030971 ISO
    transcription factor binding GO:0008134 IPI
    protein binding GO:0005515 IPI
Biological Process
    cellular response to xenobiotic stimulus GO:0071466 IDA
    base-excision repair, gap-filling GO:0006287 IDA
    cellular response to DNA damage stimulus GO:0006974 IEA
    DNA repair GO:0006281 IEA
    regulation of catalytic activity GO:0050790 IBA
    leading strand elongation GO:0006272 IBA
    DNA replication GO:0006260 IEA
    response to lipid GO:0033993 ISO
    cellular response to UV GO:0034644 ISO
    positive regulation of DNA repair GO:0045739 ISO
    translesion synthesis GO:0019985 ISO
    mismatch repair GO:0006298 ISO
    positive regulation of deoxyribonuclease activity GO:0032077 ISO
    negative regulation of transcription from RNA polymerase II promoter GO:0000122 IDA
    positive regulation of DNA replication GO:0045740 ISO
    regulation of DNA replication GO:0006275 IEA
Subcellular Localization
    nuclear lamina GO:0005652 IDA
    nucleus GO:0005634 ISS
    nucleoplasm GO:0005654 ISO
    nuclear replication fork GO:0043596 ISO
    extracellular exosome GO:0070062 ISO
    PCNA-p21 complex GO:0070557 ISO
    intracellular GO:0005622 IDA
    cyclin-dependent protein kinase holoenzyme complex GO:0000307 IPI
    cytoplasm GO:0005737 ISO
    replication fork GO:0005657 IDA
    centrosome GO:0005813 ISO
    PCNA complex GO:0043626 ISO
 Experiment description of studies that identified Pcna in exosomes
1
Experiment ID 26
ISEV standards
✔
EM
EV Biophysical techniques
✔
Alix|GAPDH|HSP70
EV Cytosolic markers
✔
CD9
EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19109410    
Organism Mus musculus
Experiment description Proteomic and immunologic analyses of brain tumor exosomes.
Authors Graner MW, Alzate O, Dechkovskaia AM, Keene JD, Sampson JH, Mitchell DA, Bigner DD
Journal name FASEB
Publication year 2008
Sample Brain cancer cells
Sample name SMA560vIII
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.13-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [MALDI TOF/TOF]
Western blotting
2
Experiment ID 210
ISEV standards
✔
EM
EV Biophysical techniques
✘ EV Cytosolic markers
✔
CD81|FLOT1
EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23260141    
Organism Mus musculus
Experiment description Exosomes Mediate Stromal Mobilization of Autocrine Wnt-PCP Signaling in Breast Cancer Cell Migration.
Authors Luga V, Zhang L, Viloria-Petit AM, Ogunjimi AA, Inanlou MR, Chiu E, Buchanan M, Hosein AN, Basik M, Wrana JL.
Journal name Cell
Publication year 2012
Sample Fibroblasts
Sample name Normal-Fibroblasts (L cells)
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 188
ISEV standards
✔
EM
EV Biophysical techniques
✔
GAPDH|UCHL1|HSP90
EV Cytosolic markers
✘ EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19351151    
Organism Mus musculus
Experiment description Characterization of vesicles secreted from insulinoma NIT-1 cells.
Authors Lee HS, Jeong J, Lee KJ.
Journal name J Proteome Res
Publication year 2009
Sample Pancreatic cells
Sample name Pancreatic beta cell (NIT-1)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for Pcna
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Rev1l  
Affinity Capture-Western Mus musculus
Co-purification Mus musculus
2 Polb  
Affinity Capture-Western Mus musculus
Two-hybrid Mus musculus
View the network image/svg+xml



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