Gene description for USP32
Gene name ubiquitin specific peptidase 32
Gene symbol USP32
Other names/aliases NY-REN-60
USP10
Species Homo sapiens
 Database cross references - USP32
ExoCarta ExoCarta_84669
Vesiclepedia VP_84669
Entrez Gene 84669
HGNC 19143
MIM 607740
UniProt Q8NFA0  
 USP32 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
 Gene ontology annotations for USP32
Molecular Function
    cysteine-type deubiquitinase activity GO:0004843 IBA
    cysteine-type deubiquitinase activity GO:0004843 IDA
    calcium ion binding GO:0005509 IEA
    protein binding GO:0005515 IPI
Biological Process
    proteolysis GO:0006508 IEA
    protein deubiquitination GO:0016579 TAS
    positive regulation of TORC1 signaling GO:1904263 IMP
Subcellular Localization
    Golgi membrane GO:0000139 IEA
    Golgi apparatus GO:0005794 IBA
    Golgi apparatus GO:0005794 IDA
    Golgi apparatus GO:0005794 IDA
    cytosol GO:0005829 IDA
 Experiment description of studies that identified USP32 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for USP32
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 USP6  
Affinity Capture-MS Homo sapiens
2 VPS35 55737
Affinity Capture-MS Homo sapiens
3 GJD3  
Proximity Label-MS Homo sapiens
4 SNX3 8724
Affinity Capture-MS Homo sapiens
5 TRIM3 10612
Two-hybrid Homo sapiens
6 LAMP3  
Proximity Label-MS Homo sapiens
7 ATG9A 79065
Proximity Label-MS Homo sapiens
8 MAPRE2 10982
Affinity Capture-MS Homo sapiens
9 ABCF3 55324
Affinity Capture-MS Homo sapiens
10 MID1IP1  
Affinity Capture-MS Homo sapiens
11 UBC 7316
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
12 LAMP2 3920
Proximity Label-MS Homo sapiens
13 CAND1 55832
Affinity Capture-MS Homo sapiens
14 PTPN1 5770
Affinity Capture-MS Homo sapiens
15 ANKRD29  
Two-hybrid Homo sapiens
16 B3GAT1  
Proximity Label-MS Homo sapiens
17 SRC 6714
Affinity Capture-MS Homo sapiens
18 ABCD3 5825
Affinity Capture-MS Homo sapiens
19 SLC31A1 1317
Affinity Capture-MS Homo sapiens
20 C3orf52  
Affinity Capture-MS Homo sapiens
21 MRPL39 54148
Affinity Capture-MS Homo sapiens
22 RAB6B 51560
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
23 DVL2 1856
Affinity Capture-MS Homo sapiens
24 LAMTOR1 55004
Proximity Label-MS Homo sapiens
25 YWHAB 7529
Affinity Capture-MS Homo sapiens
26 GTF2I 2969
Affinity Capture-MS Homo sapiens
27 FARSA 2193
Affinity Capture-MS Homo sapiens
28 ZDHHC17 23390
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
29 EIF2AK2 5610
Affinity Capture-MS Homo sapiens
30 MANSC1 54682
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
31 CX3CL1  
Affinity Capture-MS Homo sapiens
32 TUBA1A 7846
Affinity Capture-MS Homo sapiens
33 BAG6 7917
Affinity Capture-MS Homo sapiens
34 PDXDC1 23042
Affinity Capture-MS Homo sapiens
35 RAP1B 5908
Affinity Capture-MS Homo sapiens
36 ANKHD1 54882
Affinity Capture-MS Homo sapiens
37 RIN1 9610
Affinity Capture-MS Homo sapiens
38 SYNCRIP 10492
Affinity Capture-MS Homo sapiens
39 RDH11 51109
Affinity Capture-MS Homo sapiens
40 MEOX2  
Two-hybrid Homo sapiens
41 SMC1A 8243
Affinity Capture-MS Homo sapiens
42 Pcna 18538
Affinity Capture-MS Mus musculus
43 NFKB2 4791
Affinity Capture-MS Homo sapiens
44 MAPK8 5599
Affinity Capture-MS Homo sapiens
45 UBXN6 80700
Affinity Capture-MS Homo sapiens
46 TTLL12 23170
Affinity Capture-MS Homo sapiens
47 TRIP13 9319
Affinity Capture-MS Homo sapiens
48 USP4 7375
Affinity Capture-MS Homo sapiens
49 USP32P2  
Affinity Capture-MS Homo sapiens
50 CD83  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
51 CUL3 8452
Affinity Capture-MS Homo sapiens
52 HSPA14 51182
Affinity Capture-MS Homo sapiens
53 MTNR1A  
Two-hybrid Homo sapiens
54 LRRC47 57470
Affinity Capture-MS Homo sapiens
55 METTL21B  
Affinity Capture-MS Homo sapiens
56 ENTPD7  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
57 TAGLN 6876
Affinity Capture-MS Homo sapiens
58 COASY 80347
Affinity Capture-MS Homo sapiens
59 FDFT1 2222
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
60 KCNE3  
Affinity Capture-MS Homo sapiens
61 MYC  
Affinity Capture-MS Homo sapiens
62 DIS3 22894
Affinity Capture-MS Homo sapiens
63 RAB9A 9367
Proximity Label-MS Homo sapiens
64 HDLBP 3069
Affinity Capture-MS Homo sapiens
65 RHOB 388
Proximity Label-MS Homo sapiens
66 TPCN2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
67 KRT19 3880
Affinity Capture-MS Homo sapiens
68 CHUK 1147
Affinity Capture-MS Homo sapiens
69 RAB7A 7879
Proximity Label-MS Homo sapiens
70 UBXN1 51035
Affinity Capture-MS Homo sapiens
71 AQP9  
Affinity Capture-MS Homo sapiens
72 TRIM46 80128
Two-hybrid Homo sapiens
73 RGL3  
Affinity Capture-MS Homo sapiens
74 KRT18 3875
Affinity Capture-MS Homo sapiens
75 ANKFY1 51479
Affinity Capture-MS Homo sapiens
76 ERP44 23071
Affinity Capture-MS Homo sapiens
77 ZEB1  
Affinity Capture-MS Homo sapiens
78 NME4 4833
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
79 VCPIP1 80124
Affinity Capture-MS Homo sapiens
80 IKBKG 8517
Affinity Capture-MS Homo sapiens
81 DNAAF5 54919
Affinity Capture-MS Homo sapiens
82 EFNB1 1947
Affinity Capture-MS Homo sapiens
83 OXSR1 9943
Affinity Capture-MS Homo sapiens
84 DDX58 23586
Affinity Capture-RNA Homo sapiens
85 CDK1 983
Affinity Capture-MS Homo sapiens
86 TRIP12 9320
Affinity Capture-MS Homo sapiens
87 PPP4R1L  
Affinity Capture-MS Homo sapiens
88 KRT8 3856
Affinity Capture-MS Homo sapiens
89 NAB2 4665
Affinity Capture-MS Homo sapiens
90 GAPVD1 26130
Affinity Capture-MS Homo sapiens
91 SORT1 6272
Affinity Capture-MS Homo sapiens
92 RAB11A 8766
Proximity Label-MS Homo sapiens
93 ALDH3B1 221
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
94 TRIM74  
Two-hybrid Homo sapiens
95 HSPA12A 259217
Affinity Capture-MS Homo sapiens
96 RBCK1  
Two-hybrid Homo sapiens
97 RNF144A  
Two-hybrid Homo sapiens
View the network image/svg+xml
 Pathways in which USP32 is involved
No pathways found





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