Gene description for NFKB2
Gene name nuclear factor of kappa light polypeptide gene enhancer in B-cells 2 (p49/p100)
Gene symbol NFKB2
Other names/aliases CVID10
H2TF1
LYT-10
LYT10
NF-kB2
p100
p52
Species Homo sapiens
 Database cross references - NFKB2
ExoCarta ExoCarta_4791
Entrez Gene 4791
HGNC 7795
MIM 164012
UniProt Q00653  
 NFKB2 identified in exosomes derived from the following tissue/cell type
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
 Gene ontology annotations for NFKB2
Molecular Function
    sequence-specific DNA binding transcription factor activity GO:0003700 TAS
    chromatin binding GO:0003682 IBA
    RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription GO:0001077 IDA
    transcription coactivator activity GO:0003713 TAS
    RNA polymerase II core promoter proximal region sequence-specific DNA binding GO:0000978 IDA
    protein binding GO:0005515 IPI
Biological Process
    positive regulation of transcription from RNA polymerase II promoter GO:0045944 IDA
    toll-like receptor signaling pathway GO:0002224 TAS
    toll-like receptor TLR1:TLR2 signaling pathway GO:0038123 TAS
    negative regulation of transcription from RNA polymerase II promoter GO:0000122 IBA
    rhythmic process GO:0048511 IEA
    TRIF-dependent toll-like receptor signaling pathway GO:0035666 TAS
    germinal center formation GO:0002467 IEA
    inflammatory response GO:0006954 IBA
    toll-like receptor 2 signaling pathway GO:0034134 TAS
    I-kappaB kinase/NF-kappaB signaling GO:0007249 IBA
    MyD88-dependent toll-like receptor signaling pathway GO:0002755 TAS
    toll-like receptor TLR6:TLR2 signaling pathway GO:0038124 TAS
    toll-like receptor 10 signaling pathway GO:0034166 TAS
    MyD88-independent toll-like receptor signaling pathway GO:0002756 TAS
    NIK/NF-kappaB signaling GO:0038061 IBA
    toll-like receptor 9 signaling pathway GO:0034162 TAS
    extracellular matrix organization GO:0030198 IEA
    toll-like receptor 3 signaling pathway GO:0034138 TAS
    follicular dendritic cell differentiation GO:0002268 IEA
    innate immune response GO:0045087 TAS
    positive regulation of NF-kappaB transcription factor activity GO:0051092 TAS
    transcription, DNA-templated GO:0006351 IEA
    toll-like receptor 4 signaling pathway GO:0034142 TAS
    response to cytokine GO:0034097 IBA
    regulation of transcription, DNA-templated GO:0006355 IDA
    toll-like receptor 5 signaling pathway GO:0034146 TAS
    stimulatory C-type lectin receptor signaling pathway GO:0002223 TAS
    positive regulation of type I interferon production GO:0032481 TAS
    spleen development GO:0048536 IEA
Subcellular Localization
    nucleoplasm GO:0005654 TAS
    cytoplasm GO:0005737 IDA
    Bcl3/NF-kappaB2 complex GO:0033257 IDA
    cytosol GO:0005829 TAS
    nucleus GO:0005634 IDA
 Experiment description of studies that identified NFKB2 in exosomes
1
Experiment ID 211
ISEV standards
EM
EV Biophysical techniques
TSG101|Alix
EV Cytosolic markers
EpCAM|TFRC
EV Membrane markers
cytochrome c|GOLGA2
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
2
Experiment ID 212
ISEV standards
CEM
EV Biophysical techniques
TSG101|Alix
EV Cytosolic markers
EpCAM|TFRC
EV Membrane markers
Cytochrome C|GOLGA2
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for NFKB2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 USP2  
Affinity Capture-MS Homo sapiens
2 MAP3K14  
Affinity Capture-Western Homo sapiens
3 BTRC 8945
Affinity Capture-Western Homo sapiens
4 RPL6 6128
Affinity Capture-MS Homo sapiens
5 RPL30 6156
Affinity Capture-MS Homo sapiens
6 DDX3X 1654
Affinity Capture-MS Homo sapiens
7 SP1  
Reconstituted Complex Homo sapiens
8 REL 5966
Invivo Homo sapiens
Affinity Capture-MS Homo sapiens
9 MEN1 4221
Reconstituted Complex Homo sapiens
10 IKBKG 8517
Affinity Capture-MS Homo sapiens
11 NR3C1 2908
Reconstituted Complex Homo sapiens
12 NFKBIE  
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
13 RELA  
Invivo Homo sapiens
Affinity Capture-MS Homo sapiens
14 BCL3  
Invivo Homo sapiens
Invivo Homo sapiens
Co-localization Homo sapiens
15 MAP3K8  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
16 TNIP2  
Affinity Capture-MS Homo sapiens
17 TBK1 29110
Affinity Capture-MS Homo sapiens
18 RPS13 6207
Affinity Capture-MS Homo sapiens
19 NKRF 55922
Invitro Homo sapiens
20 SEC16A 9919
Affinity Capture-MS Homo sapiens
21 RELB  
Affinity Capture-MS Homo sapiens
Invivo Homo sapiens
Invitro Homo sapiens
Affinity Capture-MS Homo sapiens
Co-localization Homo sapiens
22 NFKB1 4790
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
23 FBXW7  
Affinity Capture-MS Homo sapiens
24 NFKB2 4791
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
25 TSC22D3  
Invivo Homo sapiens
Invitro Homo sapiens
26 GLG1 2734
Affinity Capture-MS Homo sapiens
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