Gene description for IKBKB
Gene name inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase beta
Gene symbol IKBKB
Other names/aliases IKK-beta
IKK2
IKKB
IMD15
NFKBIKB
Species Homo sapiens
 Database cross references - IKBKB
ExoCarta ExoCarta_3551
Vesiclepedia VP_3551
Entrez Gene 3551
HGNC 5960
MIM 603258
UniProt O14920  
 IKBKB identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Mesenchymal stem cells 36408942    
Thymus 23844026    
 Gene ontology annotations for IKBKB
Molecular Function
    scaffold protein binding GO:0097110 IDA
    protein heterodimerization activity GO:0046982 IDA
    protein binding GO:0005515 IPI
    IkappaB kinase activity GO:0008384 TAS
    ATP binding GO:0005524 IEA
    protein serine/threonine kinase activity GO:0004674 TAS
    protein kinase binding GO:0019901 IPI
    protein homodimerization activity GO:0042803 IDA
    protein kinase activity GO:0004672 IDA
Biological Process
    cellular response to tumor necrosis factor GO:0071356 IDA
    stimulatory C-type lectin receptor signaling pathway GO:0002223 TAS
    serine phosphorylation of STAT protein GO:0042501 IDA
    positive regulation of I-kappaB kinase/NF-kappaB signaling GO:0043123 TAS
    positive regulation of type I interferon production GO:0032481 TAS
    B cell homeostasis GO:0001782 IEA
    positive regulation of sodium ion transport GO:0010765 IEA
    toll-like receptor 2 signaling pathway GO:0034134 TAS
    toll-like receptor 4 signaling pathway GO:0034142 TAS
    inflammatory response GO:0006954 TAS
    negative regulation of apoptotic process GO:0043066 TAS
    nucleotide-binding oligomerization domain containing signaling pathway GO:0070423 TAS
    Fc-epsilon receptor signaling pathway GO:0038095 TAS
    toll-like receptor signaling pathway GO:0002224 TAS
    I-kappaB phosphorylation GO:0007252 TAS
    toll-like receptor 9 signaling pathway GO:0034162 TAS
    response to virus GO:0009615 TAS
    positive regulation of transcription from RNA polymerase II promoter GO:0045944 IDA
    TRIF-dependent toll-like receptor signaling pathway GO:0035666 TAS
    positive regulation of NF-kappaB transcription factor activity GO:0051092 TAS
    nucleotide-binding domain, leucine rich repeat containing receptor signaling pathway GO:0035872 TAS
    toll-like receptor TLR6:TLR2 signaling pathway GO:0038124 TAS
    toll-like receptor 10 signaling pathway GO:0034166 TAS
    I-kappaB kinase/NF-kappaB signaling GO:0007249 TAS
    MyD88-dependent toll-like receptor signaling pathway GO:0002755 TAS
    innate immune response GO:0045087 TAS
    positive regulation of cation channel activity GO:2001259 IEA
    MyD88-independent toll-like receptor signaling pathway GO:0002756 TAS
    T cell receptor signaling pathway GO:0050852 TAS
    positive regulation of transcription, DNA-templated GO:0045893 NAS
    toll-like receptor 5 signaling pathway GO:0034146 TAS
    toll-like receptor 3 signaling pathway GO:0034138 TAS
    protein phosphorylation GO:0006468 NAS
    neurotrophin TRK receptor signaling pathway GO:0048011 TAS
    toll-like receptor TLR1:TLR2 signaling pathway GO:0038123 TAS
Subcellular Localization
    CD40 receptor complex GO:0035631 ISS
    cytosol GO:0005829 TAS
    IkappaB kinase complex GO:0008385 TAS
    membrane raft GO:0045121 IEA
    cytoplasm GO:0005737 IDA
    cytoplasmic side of plasma membrane GO:0009898 ISS
    nucleus GO:0005634 IEA
 Experiment description of studies that identified IKBKB in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 488
MISEV standards
✔
EM
EV Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
EV Enriched markers
✔
CANX
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
6
Experiment ID 217
MISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|CD81|CD9|CD63
EV Enriched markers
✘ EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O.
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for IKBKB
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 TNFAIP3 7128
Invivo Homo sapiens
Invitro Homo sapiens
2 IKBKAP 8518
Invivo Homo sapiens
Invitro Homo sapiens
3 TNFRSF1A 7132
Affinity Capture-Western Homo sapiens
4 MAP3K14  
Invivo Homo sapiens
5 BTRC 8945
Affinity Capture-Western Homo sapiens
6 TRAF3IP2  
Invivo Homo sapiens
7 TRAF2 7186
Reconstituted Complex Homo sapiens
8 CFLAR  
Invivo Homo sapiens
9 PPM1B 5495
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
10 CTNNB1 1499
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
11 CDC37 11140
Co-purification Homo sapiens
Reconstituted Complex Homo sapiens
12 IKBKG 8517
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Invitro Homo sapiens
Co-purification Homo sapiens
13 CASP8 841
Invivo Homo sapiens
14 IRAK1  
Affinity Capture-Western Homo sapiens
15 MAP3K1 4214
Invivo Homo sapiens
16 PRKCQ 5588
Affinity Capture-Western Homo sapiens
17 PRKDC 5591
Biochemical Activity Homo sapiens
18 FANCA  
Invivo Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Co-purification Homo sapiens
19 MAP3K13 9175
Affinity Capture-Western Homo sapiens
20 PRDX3 10935
Affinity Capture-Western Homo sapiens
21 MAP3K11 4296
Invivo Homo sapiens
22 EIF2AK2 5610
Invivo Homo sapiens
Invitro Homo sapiens
23 NFKB1 4790
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
24 NCOA3  
Invitro Homo sapiens
25 IRS1 3667
Affinity Capture-Western Homo sapiens
26 CHUK 1147
Co-purification Homo sapiens
Affinity Capture-Western Homo sapiens
Co-purification Homo sapiens
27 TRPC4AP  
Affinity Capture-Western Homo sapiens
View the network image/svg+xml



Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here