Gene description for Pebp1
Gene name phosphatidylethanolamine binding protein 1
Gene symbol Pebp1
Other names/aliases HCNP
Pbp
Rkip
Species Rattus norvegicus
 Database cross references - Pebp1
ExoCarta ExoCarta_29542
Vesiclepedia VP_29542
Entrez Gene 29542
UniProt P31044  
 Pebp1 identified in exosomes derived from the following tissue/cell type
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
Urine 20535238    
 Gene ontology annotations for Pebp1
Molecular Function
    serine-type endopeptidase inhibitor activity GO:0004867 IEA
    signaling receptor binding GO:0005102 IDA
    ATP binding GO:0005524 IDA
    lipid binding GO:0008289 TAS
    enzyme binding GO:0019899 ISO
    kinase binding GO:0019900 IPI
    protein kinase binding GO:0019901 IPI
    protein kinase binding GO:0019901 ISO
    receptor serine/threonine kinase binding GO:0033612 IPI
    mitogen-activated protein kinase binding GO:0051019 IDA
Biological Process
    MAPK cascade GO:0000165 IDA
    negative regulation of protein phosphorylation GO:0001933 IMP
    regulation of the force of heart contraction GO:0002026 IDA
    response to oxidative stress GO:0006979 IEP
    spermatid development GO:0007286 IEP
    spermatid development GO:0007286 TAS
    response to xenobiotic stimulus GO:0009410 IEP
    response to toxic substance GO:0009636 IEP
    response to activity GO:0014823 IEP
    hippocampus development GO:0021766 IEP
    eating behavior GO:0042755 IEP
    negative regulation of MAPK cascade GO:0043409 IBA
    negative regulation of MAPK cascade GO:0043409 IMP
    response to ethanol GO:0045471 IEP
    positive regulation of mitotic nuclear division GO:0045840 IMP
    sperm capacitation GO:0048240 ISO
    response to corticosterone GO:0051412 IEP
    response to cAMP GO:0051591 IEP
    response to calcium ion GO:0051592 IEP
    response to electrical stimulus GO:0051602 IEP
    positive regulation of acetylcholine metabolic process GO:0060409 IDA
    positive regulation of cAMP/PKA signal transduction GO:0141163 IMP
    positive regulation of acetylcholine biosynthetic process GO:1905923 IDA
Subcellular Localization
    extracellular space GO:0005615 IDA
    mitochondrial outer membrane GO:0005741 IDA
    synaptic vesicle GO:0008021 IDA
    cell surface GO:0009986 IDA
    cell surface GO:0009986 ISO
    neuronal cell body GO:0043025 IDA
    axon terminus GO:0043679 IDA
    apical part of cell GO:0045177 IDA
 Experiment description of studies that identified Pebp1 in exosomes
1
Experiment ID 95
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
2
Experiment ID 97
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D4 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
3
Experiment ID 98
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
4
Experiment ID 192
MISEV standards
✔
CEM
Biophysical techniques
✔
TSG101|HSP70|CD63|CD81|FLOT
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20535238    
Organism Rattus norvegicus
Experiment description Candidate biomarkers in exosome-like vesicles purified from rat and mouse urine samples.
Authors "Conde-Vancells J, Rodriguez-Suarez E, Gonzalez E, Berisa A, Gil D, Embade N, Valle M, Luka Z, Elortza F, Wagner C, Lu SC, Mato JM, Falcon-Perez M."
Journal name Proteomics Clin Appl
Publication year 2010
Sample Urine
Sample name Urine - Rats of liver injury
Isolation/purification methods Differential centrifugation
Filtration
UltracentrifugationSucrose cushion
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for Pebp1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Map3k7  
Affinity Capture-Western Mus musculus
2 MAP3K14  
Affinity Capture-Western Homo sapiens
Phenotypic Suppression Homo sapiens
3 Prkcz  
Biochemical Activity Rattus norvegicus
Reconstituted Complex Rattus norvegicus
4 Prkcg  
Biochemical Activity Rattus norvegicus
5 Prkca  
Biochemical Activity Rattus norvegicus
6 IKBKB 3551
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
7 CHUK 1147
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
8 Prkcb  
Biochemical Activity Rattus norvegicus
9 Raf1  
Reconstituted Complex Rattus norvegicus
Affinity Capture-Western Rattus norvegicus
10 MAP3K7  
Affinity Capture-Western Homo sapiens
View the network image/svg+xml



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