Gene description for PRKCE
Gene name protein kinase C, epsilon
Gene symbol PRKCE
Other names/aliases PKCE
nPKC-epsilon
Species Homo sapiens
 Database cross references - PRKCE
ExoCarta ExoCarta_5581
Vesiclepedia VP_5581
Entrez Gene 5581
HGNC 9401
MIM 176975
UniProt Q02156  
 PRKCE identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
 Gene ontology annotations for PRKCE
Molecular Function
    actin monomer binding GO:0003785 ISS
    enzyme activator activity GO:0008047 IMP
    signal transducer activity GO:0004871 TAS
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IEA
    metal ion binding GO:0046872 IEA
    protein serine/threonine kinase activity GO:0004674 IDA
    receptor activator activity GO:0030546 IEA
    enzyme binding GO:0019899 IPI
    protein kinase C activity GO:0004697 IDA
    ethanol binding GO:0035276 IEA
    calcium-independent protein kinase C activity GO:0004699 IEA
    14-3-3 protein binding GO:0071889 IEA
Biological Process
    positive regulation of lipid catabolic process GO:0050996 IEA
    activation of phospholipase C activity GO:0007202 TAS
    negative regulation of sodium ion transmembrane transporter activity GO:2000650 TAS
    regulation of peptidyl-tyrosine phosphorylation GO:0050730 IEA
    apoptotic process GO:0006915 TAS
    response to morphine GO:0043278 IEA
    positive regulation of cytokinesis GO:0032467 IMP
    positive regulation of epithelial cell migration GO:0010634 IMP
    positive regulation of cell-substrate adhesion GO:0010811 IEA
    peptidyl-serine phosphorylation GO:0018105 IDA
    neurotrophin TRK receptor signaling pathway GO:0048011 TAS
    cell division GO:0051301 IEA
    positive regulation of insulin secretion GO:0032024 IEA
    lipopolysaccharide-mediated signaling pathway GO:0031663 ISS
    cellular response to ethanol GO:0071361 IEA
    epidermal growth factor receptor signaling pathway GO:0007173 TAS
    macrophage activation involved in immune response GO:0002281 IEA
    protein phosphorylation GO:0006468 IDA
    regulation of insulin secretion involved in cellular response to glucose stimulus GO:0061178 IEA
    positive regulation of actin filament polymerization GO:0030838 ISS
    locomotory exploration behavior GO:0035641 IEA
    cell adhesion GO:0007155 IEA
    platelet activation GO:0030168 TAS
    intracellular signal transduction GO:0035556 IEA
    Fc-gamma receptor signaling pathway involved in phagocytosis GO:0038096 TAS
    positive regulation of wound healing GO:0090303 IMP
    positive regulation of receptor activity GO:2000273 IEA
    signal transduction GO:0007165 TAS
    positive regulation of fibroblast migration GO:0010763 ISS
    fibroblast growth factor receptor signaling pathway GO:0008543 TAS
    positive regulation of I-kappaB kinase/NF-kappaB signaling GO:0043123 IEA
    release of sequestered calcium ion into cytosol GO:0051209 IEA
    innate immune response GO:0045087 TAS
    positive regulation of MAPK cascade GO:0043410 IEA
    positive regulation of synaptic transmission, GABAergic GO:0032230 IEA
    cellular response to hypoxia GO:0071456 IEA
    blood coagulation GO:0007596 TAS
    positive regulation of cellular glucuronidation GO:2001031 IMP
    positive regulation of mucus secretion GO:0070257 IEA
    cell cycle GO:0007049 IEA
    TRAM-dependent toll-like receptor 4 signaling pathway GO:0035669 ISS
Subcellular Localization
    cytosol GO:0005829 TAS
    mitochondrion GO:0005739 IEA
    endoplasmic reticulum GO:0005783 IDA
    Golgi apparatus GO:0005794 ISS
    cell periphery GO:0071944 ISS
    nucleus GO:0005634 IEA
    plasma membrane GO:0005886 TAS
    cytoplasm GO:0005737 IDA
    cytoskeleton GO:0005856 IEA
    perinuclear region of cytoplasm GO:0048471 ISS
 Experiment description of studies that identified PRKCE in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for PRKCE
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 HK2 3099
Affinity Capture-Western Homo sapiens
2 KRT18 3875
Invivo Homo sapiens
3 SNCA 6622
Invivo Homo sapiens
4 GNA12 2768
Biochemical Activity Homo sapiens
5 GAD2  
Biochemical Activity Homo sapiens
6 RASGRP3  
Biochemical Activity Homo sapiens
7 CNN1 1264
Invivo Homo sapiens
8 GJA1 2697
Affinity Capture-Western Homo sapiens
9 MAPK1 5594
Affinity Capture-Western Homo sapiens
10 ACTC1 70
Invivo Homo sapiens
11 GAD1  
Biochemical Activity Homo sapiens
12 AFAP1 60312
Invitro Homo sapiens
13 ACTA1 58
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
14 CFTR  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
15 PDLIM5 10611
Two-hybrid Homo sapiens
16 SLC25A4 291
Affinity Capture-Western Homo sapiens
17 MYH9 4627
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
18 PIK3CB 5291
Invivo Homo sapiens
19 COPB2 9276
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
20 RAF1 5894
Affinity Capture-Western Homo sapiens
21 GNA13 10672
Biochemical Activity Homo sapiens
22 CENTA1  
Biochemical Activity Homo sapiens
23 SRC 6714
Invitro Homo sapiens
24 GNB2L1 10399
Far Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
25 VDAC1 7416
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Biochemical Activity Homo sapiens
26 TIAM1  
Invitro Homo sapiens
27 KRT1 3848
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
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