Gene description for ACTA1
Gene name actin, alpha 1, skeletal muscle
Gene symbol ACTA1
Other names/aliases ACTA
ASMA
CFTD
CFTD1
CFTDM
MPFD
NEM1
NEM2
NEM3
Species Homo sapiens
 Database cross references - ACTA1
ExoCarta ExoCarta_58
Vesiclepedia VP_58
Entrez Gene 58
HGNC 129
MIM 102610
UniProt P68133  
 ACTA1 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Breast cancer cells 35918900    
Breast cancer cells 35918900    
Chondrocytes 35931686    
Chondrocytes 35931686    
Chondrocytes 35931686    
Chondrocytes 35931686    
Colorectal cancer cells 17956143    
Colorectal cancer cells 19837982    
Glioma cells 19109410    
Medulloblastoma cells 19109410    
Melanoma cells 25950383    
Melanoma cells 25950383    
Melanoma cells 25950383    
Melanoma cells 25950383    
Melanoma cells 25950383    
Mesenchymal stem cells Unpublished / Not applicable
Nasopharyngeal carcinoma cells 21059916    
Neuroblastoma cells 25944692    
Platelets 25332113    
Platelets 25332113    
Platelets 25332113    
Prostate cancer cells 22723089    
Prostate cancer cells 22723089    
Prostate cancer cells 22723089    
Prostate cancer cells 22723089    
Prostate cancer cells 22723089    
Prostate cancer cells 22723089    
Prostate cancer cells 22723089    
Prostate cancer cells 22723089    
Prostate cancer cells 22723089    
Prostate cancer cells 22723089    
Prostate cancer cells 22723089    
Prostate cancer cells 25844599    
Prostate cancer cells 25844599    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
Saliva 19199708    
Squamous carcinoma cells 20124223    
Urine 21557262    
Urine 21595033    
Urine 21595033    
Urine 21595033    
 Gene ontology annotations for ACTA1
Molecular Function
    structural constituent of cytoskeleton GO:0005200 TAS
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 TAS
    hydrolase activity GO:0016787 IEA
    myosin binding GO:0017022 TAS
    ADP binding GO:0043531 TAS
Biological Process
    muscle contraction GO:0006936 TAS
    response to mechanical stimulus GO:0009612 IEA
    positive regulation of gene expression GO:0010628 ISS
    skeletal muscle thin filament assembly GO:0030240 IBA
    skeletal muscle thin filament assembly GO:0030240 IMP
    cellular response to potassium ion GO:0035865 IEA
    skeletal muscle fiber adaptation GO:0043503 IEA
    response to steroid hormone GO:0048545 IEA
    skeletal muscle fiber development GO:0048741 ISS
    mesenchyme migration GO:0090131 ISS
Subcellular Localization
    stress fiber GO:0001725 IBA
    stress fiber GO:0001725 IDA
    extracellular space GO:0005615 HDA
    cytosol GO:0005829 TAS
    striated muscle thin filament GO:0005865 IBA
    striated muscle thin filament GO:0005865 IDA
    actin filament GO:0005884 IBA
    actin filament GO:0005884 IDA
    actin cytoskeleton GO:0015629 IBA
    actin cytoskeleton GO:0015629 IMP
    sarcomere GO:0030017 IDA
    lamellipodium GO:0030027 ISS
    filopodium GO:0030175 ISS
    cell body GO:0044297 ISS
    extracellular exosome GO:0070062 HDA
    blood microparticle GO:0072562 HDA
 Experiment description of studies that identified ACTA1 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 1334
MISEV standards
Biophysical techniques
✔
CD9
Enriched markers
✔
ACTB
Negative markers
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35918900    
Organism Homo sapiens
Experiment description Characterization of protein complexes in extracellular vesicles by intact extracellular vesicle crosslinking mass spectrometry (iEVXL)
Authors "Bauzá-Martinez J, Armony G, Pronker MF, Wu W. "
Journal name J Extracell Vesicles
Publication year 2022
Sample Breast cancer cells
Sample name LM2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectometry
6
Experiment ID 1336
MISEV standards
Biophysical techniques
✔
CD9
Enriched markers
✔
ACTB
Negative markers
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35918900    
Organism Homo sapiens
Experiment description Characterization of protein complexes in extracellular vesicles by intact extracellular vesicle crosslinking mass spectrometry (iEVXL)
Authors "Bauzá-Martinez J, Armony G, Pronker MF, Wu W. "
Journal name J Extracell Vesicles
Publication year 2022
Sample Breast cancer cells
Sample name LM2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectometry
7
Experiment ID 494
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD151|CD63|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|SDCBP|TFRC|TSG101
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name Osteoarthritic cartilage
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
8
Experiment ID 496
MISEV standards
✘ Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|SDCBP|TFRC|TSG101
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name Healthy cartilage
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
9
Experiment ID 497
MISEV standards
✘ Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
10
Experiment ID 498
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
11
Experiment ID 20
MISEV standards
✔
EM
Biophysical techniques
✔
HSP90|CD63|CD81|LAMP1
Enriched markers
✔
GOLGA2|cytochrome c
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 17956143    
Organism Homo sapiens
Experiment description Proteomic analysis of microvesicles derived from human colorectal cancer cells.
Authors "Choi DS, Lee JM, Park GW, Lim HW, Bang JY, Kim YK, Kwon KH, Kwon HJ, Kim KP, Gho YS"
Journal name JPR
Publication year 2007
Sample Colorectal cancer cells
Sample name HT29
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Diafiltration
Flotation density 1.16 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
12
Experiment ID 21
MISEV standards
✔
EM|IEM
Biophysical techniques
✔
Alix|TSG101|HSP70|CD63
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method
Mass spectrometry   
PubMed ID 19837982    
Organism Homo sapiens
Experiment description Proteomic and bioinformatic analysis of immunoaffinity-purified exosomes derived from the human colon tumor cell line LIM1215.
Authors "Suresh Mathivanan, Justin W.E. Lim, Bow J. Tauro, Hong Ji, Robert L. Moritz and Richard J. Simpson"
Journal name MCP
Publication year 2009
Sample Colorectal cancer cells
Sample name LIM1215
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.10-1.12 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [Orbitrap]
Western blotting
13
Experiment ID 28
MISEV standards
✔
EM
Biophysical techniques
✔
HSP70|HSC70
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 19109410    
Organism Homo sapiens
Experiment description Proteomic and immunologic analyses of brain tumor exosomes.
Authors "Graner MW, Alzate O, Dechkovskaia AM, Keene JD, Sampson JH, Mitchell DA, Bigner DD"
Journal name FASEB
Publication year 2008
Sample Glioma cells
Sample name "X43,T
D456MG
H2159MG
D54MG
D247MG"
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
14
Experiment ID 27
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 19109410    
Organism Homo sapiens
Experiment description Proteomic and immunologic analyses of brain tumor exosomes.
Authors "Graner MW, Alzate O, Dechkovskaia AM, Keene JD, Sampson JH, Mitchell DA, Bigner DD"
Journal name FASEB
Publication year 2008
Sample Medulloblastoma cells
Sample name D283MED
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
15
Experiment ID 254
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|FLOT1|CD81
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors "Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L"
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name MNT-1
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
16
Experiment ID 255
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|FLOT1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors "Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L"
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name G1
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
17
Experiment ID 256
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|FLOT1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors "Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L"
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name 501mel
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
18
Experiment ID 259
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|FLOT1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors "Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L"
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name A375M
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
19
Experiment ID 260
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|FLOT1|CD81
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors "Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L"
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name 1205Lu
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
20
Experiment ID 126
MISEV standards
✘ Biophysical techniques
✔
GAPDH
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [LTQ-FT Ultra]
PubMed ID Unpublished / Not applicable
Organism Homo sapiens
Experiment description Mesenchymal Stem Cell Exosomes: The Future MSC-based Therapy?
Authors "Ruenn Chai Lai, Ronne Wee Yeh Yeo, Soon Sim Tan, Bin Zhang, Yijun Yin, Newman Siu Kwan Sze, Andre Choo, and Sai Kiang Lim"
Journal name Mesenchymal Stem Cell Therapy
Publication year 2011
Sample Mesenchymal stem cells
Sample name huES9.E1
Isolation/purification methods HPLC
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Antobody array
Mass spectrometry
21
Experiment ID 125
MISEV standards
✘ Biophysical techniques
✔
HSC70|GAPDH|FLOT2
Enriched markers
✔
HSPA5
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 21059916    
Organism Homo sapiens
Experiment description Human tumor virus utilizes exosomes for intercellular communication.
Authors "Meckes DG Jr, Shair KH, Marquitz AR, Kung CP, Edwards RH, Raab-Traub N."
Journal name PNAS
Publication year 2010
Sample Nasopharyngeal carcinoma cells
Sample name C666
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
22
Experiment ID 224
MISEV standards
✔
EM|AFM
Biophysical techniques
✔
Alix|TSG101|CD63|CD81
Enriched markers
✔
GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25944692    
Organism Homo sapiens
Experiment description Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes
Authors "Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S"
Journal name Oncotarget
Publication year 2015
Sample Neuroblastoma cells
Sample name SH-SY5Y
Isolation/purification methods Differential centrifugation
Ultracentrifugation
OptiPrep density gradient
Flotation density 1.10 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
23
Experiment ID 231
MISEV standards
✘ Biophysical techniques
✔
Alix|CD63|CD9
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Western blotting
Mass spectrometry
24
Experiment ID 232
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
25
Experiment ID 233
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
26
Experiment ID 136
MISEV standards
✔
EM
Biophysical techniques
✔
HSP70|HSP90|RAB5|CD9
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method
Mass spectrometry [QTOF]   
PubMed ID 22723089    
Organism Homo sapiens
Experiment description Prostate cancer cell derived exosomes
Authors "Hosseini-Beheshti E, Guns ES."
Journal name MCP
Publication year 2012
Sample Prostate cancer cells
Sample name PC3 - Rep 2
Isolation/purification methods Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QTOF]
27
Experiment ID 137
MISEV standards
✔
EM
Biophysical techniques
✔
HSP70|HSP90|RAB6|CD10
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method
Mass spectrometry [QTOF]   
PubMed ID 22723089    
Organism Homo sapiens
Experiment description Prostate cancer cell derived exosomes
Authors "Hosseini-Beheshti E, Guns ES."
Journal name MCP
Publication year 2012
Sample Prostate cancer cells
Sample name PC3 - Rep 3
Isolation/purification methods Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QTOF]
28
Experiment ID 138
MISEV standards
✔
EM
Biophysical techniques
✔
HSP70|HSP90|RAB5|LAMP2|CD9
Enriched markers
✔
HSP90B1
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method
Mass spectrometry [QTOF]   
PubMed ID 22723089    
Organism Homo sapiens
Experiment description Prostate cancer cell derived exosomes
Authors "Hosseini-Beheshti E, Guns ES."
Journal name MCP
Publication year 2012
Sample Prostate cancer cells
Sample name DU145 - Rep 2
Isolation/purification methods Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QTOF]
29
Experiment ID 139
MISEV standards
✔
EM
Biophysical techniques
✔
HSP70|HSP90|RAB5|LAMP2|CD9
Enriched markers
✔
HSP90B1
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method
Mass spectrometry [QTOF]   
PubMed ID 22723089    
Organism Homo sapiens
Experiment description Prostate cancer cell derived exosomes
Authors "Hosseini-Beheshti E, Guns ES."
Journal name MCP
Publication year 2012
Sample Prostate cancer cells
Sample name DU145 - Rep 3
Isolation/purification methods Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QTOF]
30
Experiment ID 140
MISEV standards
✔
EM
Biophysical techniques
✔
HSP70|HSP90|RAB5|LAMP2|CD9
Enriched markers
✔
HSP90B1
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method
Mass spectrometry [QTOF]   
PubMed ID 22723089    
Organism Homo sapiens
Experiment description Prostate cancer cell derived exosomes
Authors "Hosseini-Beheshti E, Guns ES."
Journal name MCP
Publication year 2012
Sample Prostate cancer cells
Sample name VCaP - Rep 2
Isolation/purification methods Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QTOF]
31
Experiment ID 141
MISEV standards
✔
EM
Biophysical techniques
✔
HSP70|HSP90|RAB5|LAMP2|CD9
Enriched markers
✔
HSP90B1
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method
Mass spectrometry [QTOF]   
PubMed ID 22723089    
Organism Homo sapiens
Experiment description Prostate cancer cell derived exosomes
Authors "Hosseini-Beheshti E, Guns ES."
Journal name MCP
Publication year 2012
Sample Prostate cancer cells
Sample name VCaP - Rep 3
Isolation/purification methods Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QTOF]
32
Experiment ID 142
MISEV standards
✔
EM
Biophysical techniques
✔
HSP70|HSP90|RAB5|LAMP2|CD9
Enriched markers
✔
HSP90B1
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method
Mass spectrometry [QTOF]   
PubMed ID 22723089    
Organism Homo sapiens
Experiment description Prostate cancer cell derived exosomes
Authors "Hosseini-Beheshti E, Guns ES."
Journal name MCP
Publication year 2012
Sample Prostate cancer cells
Sample name LNCaP - Rep 2
Isolation/purification methods Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QTOF]
33
Experiment ID 143
MISEV standards
✔
EM
Biophysical techniques
✔
HSP70|HSP90|RAB5|LAMP2|CD9
Enriched markers
✔
HSP90B1
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method
Mass spectrometry [QTOF]   
PubMed ID 22723089    
Organism Homo sapiens
Experiment description Prostate cancer cell derived exosomes
Authors "Hosseini-Beheshti E, Guns ES."
Journal name MCP
Publication year 2012
Sample Prostate cancer cells
Sample name LNCaP - Rep 3
Isolation/purification methods Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QTOF]
34
Experiment ID 144
MISEV standards
✔
EM
Biophysical techniques
✔
HSP70|HSP90|RAB5|CD9
Enriched markers
✔
HSP90B1
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method
Mass spectrometry [QTOF]   
PubMed ID 22723089    
Organism Homo sapiens
Experiment description Prostate cancer cell derived exosomes
Authors "Hosseini-Beheshti E, Guns ES."
Journal name MCP
Publication year 2012
Sample Prostate cancer cells
Sample name C4-2 - Rep 2
Isolation/purification methods Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QTOF]
35
Experiment ID 145
MISEV standards
✔
EM
Biophysical techniques
✔
HSP70|HSP90|RAB5|CD10
Enriched markers
✔
HSP90B1
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method
Mass spectrometry [QTOF]   
PubMed ID 22723089    
Organism Homo sapiens
Experiment description Prostate cancer cell derived exosomes
Authors "Hosseini-Beheshti E, Guns ES."
Journal name MCP
Publication year 2012
Sample Prostate cancer cells
Sample name C4-2 - Rep 3
Isolation/purification methods Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QTOF]
36
Experiment ID 146
MISEV standards
✔
EM
Biophysical techniques
✔
HSP70|HSP90|CD9
Enriched markers
✔
HSP90B1
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method
Mass spectrometry [QTOF]   
PubMed ID 22723089    
Organism Homo sapiens
Experiment description Prostate cancer cell derived exosomes
Authors "Hosseini-Beheshti E, Guns ES."
Journal name MCP
Publication year 2012
Sample Prostate cancer cells
Sample name RWPE - Rep 2
Isolation/purification methods Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QTOF]
37
Experiment ID 275
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|RAB5A|CD9|CD82|CD63|CD81
Enriched markers
✔
AIF
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25844599    
Organism Homo sapiens
Experiment description Molecular profiling of prostate cancer derived exosomes may reveal a predictive signature for response to docetaxel.
Authors "Kharaziha P, Chioureas D, Rutishauser D, Baltatzis G, Lennartsson L, Fonseca P, Azimi A, Hultenby K, Zubarev R, Ullen A, Yachnin J, Nilsson S, Panaretakis T."
Journal name Oncotarget
Publication year 2015
Sample Prostate cancer cells
Sample name DU145 - Docetaxel sensitive
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.12-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry/Flow cytometry/Western blotting
38
Experiment ID 274
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|RAB5A|CD9|CD82|CD63|CD81
Enriched markers
✔
AIF
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25844599    
Organism Homo sapiens
Experiment description Molecular profiling of prostate cancer derived exosomes may reveal a predictive signature for response to docetaxel.
Authors "Kharaziha P, Chioureas D, Rutishauser D, Baltatzis G, Lennartsson L, Fonseca P, Azimi A, Hultenby K, Zubarev R, Ullen A, Yachnin J, Nilsson S, Panaretakis T."
Journal name Oncotarget
Publication year 2015
Sample Prostate cancer cells
Sample name DU145 - Docetaxel resistant
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.18 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Flow cytometry
Western blotting
39
Experiment ID 834
MISEV standards
✔
EM
Biophysical techniques
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
40
Experiment ID 835
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
41
Experiment ID 66
MISEV standards
✔
IEM
Biophysical techniques
✔
TSG101|Alix|CD63|CD81
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19199708    
Organism Homo sapiens
Experiment description Proteomic analysis of human parotid gland exosomes by multidimensional protein identification technology (MudPIT).
Authors "Gonzalez-Begne M, Lu B, Han X, Hagen FK, Hand AR, Melvin JE, Yates JR"
Journal name JPR
Publication year 2009
Sample Saliva
Sample name Saliva
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
Immunoelectron Microscopy
42
Experiment ID 191
MISEV standards
✘ Biophysical techniques
✔
Alix|CD81|CD9
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20124223    
Organism Homo sapiens
Experiment description Hypoxic tumor cell modulates its microenvironment to enhance angiogenic and metastatic potential by secretion of proteins and exosomes.
Authors "Park JE, Tan HS, Datta A, Lai RC, Zhang H, Meng W, Lim SK, Sze SK."
Journal name Mol Cell Proteomics
Publication year 2010
Sample Squamous carcinoma cells
Sample name Squamous carcinoma cell (A431)
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
43
Experiment ID 114
MISEV standards
✔
IEM
Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21557262    
Organism Homo sapiens
Experiment description Proteomic identification of exosomal LRG1: A potential urinary biomarker for detecting NSCLC.
Authors "Li Y, Zhang Y, Qiu F, Qiu Z."
Journal name ELEC
Publication year 2011
Sample Urine
Sample name Urine - Lung cancer
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
44
Experiment ID 193
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|CD9
Enriched markers
✔
PHB
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21595033    
Organism Homo sapiens
Experiment description Proteomic analysis of urinary exosomes from patients of early IgA nephropathy and thin basement membrane nephropathy.
Authors "Moon PG, Lee JE, You S, Kim TK, Cho JH, Kim IS, Kwon TH, Kim CD, Park SH, Hwang D, Kim YL, Baek MC."
Journal name Proteomics
Publication year 2011
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
45
Experiment ID 194
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21595033    
Organism Homo sapiens
Experiment description Proteomic analysis of urinary exosomes from patients of early IgA nephropathy and thin basement membrane nephropathy.
Authors "Moon PG, Lee JE, You S, Kim TK, Cho JH, Kim IS, Kwon TH, Kim CD, Park SH, Hwang D, Kim YL, Baek MC."
Journal name Proteomics
Publication year 2011
Sample Urine
Sample name Urine - Patients of basement membrane nephropathy
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
46
Experiment ID 195
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21595033    
Organism Homo sapiens
Experiment description Proteomic analysis of urinary exosomes from patients of early IgA nephropathy and thin basement membrane nephropathy.
Authors "Moon PG, Lee JE, You S, Kim TK, Cho JH, Kim IS, Kwon TH, Kim CD, Park SH, Hwang D, Kim YL, Baek MC."
Journal name Proteomics
Publication year 2011
Sample Urine
Sample name Urine - Patients of early IgA nephropathy
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for ACTA1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 HNRNPH1 3187
Co-fractionation Homo sapiens
2 ACTR2 10097
Proximity Label-MS Homo sapiens
3 ISG15 9636
Affinity Capture-MS Homo sapiens
4 SCIN 85477
Reconstituted Complex Homo sapiens
5 PPP1CB 5500
Affinity Capture-MS Homo sapiens
6 ETV6  
Co-localization Homo sapiens
7 RRN3  
Affinity Capture-Western Homo sapiens
8 Tpm1 22003
Affinity Capture-MS Mus musculus
9 SHC1 6464
Affinity Capture-MS Homo sapiens
10 CCT5 22948
Co-crystal Structure Homo sapiens
11 PTPN22  
Affinity Capture-MS Homo sapiens
12 ABRA  
Two-hybrid Homo sapiens
13 ZNF324B  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
14 TPM2 7169
Reconstituted Complex Homo sapiens
15 CSNK1A1 1452
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
16 USP5 8078
Co-fractionation Homo sapiens
17 NRAP  
Reconstituted Complex Homo sapiens
18 CAP1 10487
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
19 ACTC1 70
Affinity Capture-MS Homo sapiens
Cross-Linking-MS (XL-MS) Homo sapiens
20 ANXA8 653145
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
21 UBE3A 7337
Affinity Capture-MS Homo sapiens
22 TLE3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
23 UBC 7316
Reconstituted Complex Homo sapiens
24 CCT4 10575
Co-crystal Structure Homo sapiens
25 TRIM55  
Two-hybrid Homo sapiens
26 CFL1 1072
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
27 RPS6KB2  
Affinity Capture-MS Homo sapiens
28 MTRF1  
Affinity Capture-MS Homo sapiens
29 ADAM19 8728
Affinity Capture-MS Homo sapiens
30 SLC16A10 117247
Affinity Capture-MS Homo sapiens
31 SLC39A3  
Affinity Capture-MS Homo sapiens
32 NMRAL1 57407
Affinity Capture-MS Homo sapiens
33 POTEE 445582
Cross-Linking-MS (XL-MS) Homo sapiens
34 EBP  
Affinity Capture-MS Homo sapiens
35 RDX 5962
Co-fractionation Homo sapiens
36 CCDC69 26112
Affinity Capture-MS Homo sapiens
37 MCM2 4171
Affinity Capture-MS Homo sapiens
38 KCNQ2  
Affinity Capture-Western Homo sapiens
39 ELOVL3  
Affinity Capture-MS Homo sapiens
40 AMOT 154796
Reconstituted Complex Homo sapiens
41 CDH2 1000
Affinity Capture-Western Homo sapiens
42 MRPS5 64969
Affinity Capture-MS Homo sapiens
43 KRT40  
Affinity Capture-MS Homo sapiens
44 ACTG2 72
Cross-Linking-MS (XL-MS) Homo sapiens
45 UBL4A 8266
Affinity Capture-MS Homo sapiens
46 PSMD12 5718
Co-fractionation Homo sapiens
47 POU5F1  
Affinity Capture-RNA Homo sapiens
48 DNASE1  
Co-crystal Structure Homo sapiens
49 OBSL1 23363
Affinity Capture-MS Homo sapiens
50 DMD 1756
Reconstituted Complex Homo sapiens
51 NCOA3  
Affinity Capture-MS Homo sapiens
52 WASH1 100287171
Affinity Capture-MS Homo sapiens
53 POTEI 653269
Cross-Linking-MS (XL-MS) Homo sapiens
54 CHMP1B 57132
Affinity Capture-MS Homo sapiens
55 LRSAM1 90678
Affinity Capture-MS Homo sapiens
56 HTRA2 27429
Protein-peptide Homo sapiens
Biochemical Activity Homo sapiens
57 CEP57  
Affinity Capture-MS Homo sapiens
58 SFN 2810
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
59 RPS6KB1 6198
Reconstituted Complex Homo sapiens
60 KLHL20  
Reconstituted Complex Homo sapiens
61 HDAC8 55869
Affinity Capture-Western Homo sapiens
62 MARK4  
Affinity Capture-MS Homo sapiens
63 CUL1 8454
Affinity Capture-MS Homo sapiens
64 TFCP2 7024
Affinity Capture-MS Homo sapiens
65 BRK1 55845
Co-fractionation Homo sapiens
66 Lima1  
Affinity Capture-MS Mus musculus
67 SMARCB1 6598
Affinity Capture-Western Homo sapiens
68 FGFR1 2260
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
69 NCSTN 23385
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
70 TES 26136
Affinity Capture-Western Homo sapiens
71 CCT2 10576
Reconstituted Complex Homo sapiens
72 PPM1B 5495
Affinity Capture-MS Homo sapiens
73 ACTA2 59
Affinity Capture-MS Homo sapiens
Cross-Linking-MS (XL-MS) Homo sapiens
74 PPP1CA 5499
Affinity Capture-Western Homo sapiens
75 VRK3 51231
Affinity Capture-MS Homo sapiens
76 MYO18A 399687
Affinity Capture-MS Homo sapiens
77 POLD1 5424
Affinity Capture-MS Homo sapiens
78 COASY 80347
Affinity Capture-MS Homo sapiens
79 SPTA1  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
80 HIST1H4A 8359
Affinity Capture-MS Homo sapiens
81 POTEF 728378
Cross-Linking-MS (XL-MS) Homo sapiens
82 TSPAN33 340348
Affinity Capture-MS Homo sapiens
83 FKBP6  
Affinity Capture-MS Homo sapiens
84 ERBB3 2065
Affinity Capture-MS Homo sapiens
85 CHRDL2 25884
Affinity Capture-MS Homo sapiens
86 CBL 867
Affinity Capture-MS Homo sapiens
87 RSPH6A  
Affinity Capture-MS Homo sapiens
88 HLX  
Affinity Capture-MS Homo sapiens
89 XPOT 11260
Co-fractionation Homo sapiens
90 UBAC2 337867
Affinity Capture-MS Homo sapiens
91 MAPT  
Reconstituted Complex Homo sapiens
92 YAP1 10413
Affinity Capture-MS Homo sapiens
93 MYH9 4627
Affinity Capture-MS Homo sapiens
94 VCP 7415
Affinity Capture-MS Homo sapiens
95 TRIM21 6737
Affinity Capture-MS Homo sapiens
96 NTRK1 4914
Affinity Capture-MS Homo sapiens
97 U2AF2 11338
Co-fractionation Homo sapiens
98 GC 2638
Co-crystal Structure Homo sapiens
99 PHACTR1 221692
Affinity Capture-Western Homo sapiens
100 ACTB 60
Cross-Linking-MS (XL-MS) Homo sapiens
Cross-Linking-MS (XL-MS) Homo sapiens
101 S100A2 6273
Co-fractionation Homo sapiens
102 PIK3R1 5295
Affinity Capture-Western Homo sapiens
103 DHX9 1660
Co-purification Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
104 BTK 695
Affinity Capture-MS Homo sapiens
105 DNM2 1785
Affinity Capture-MS Homo sapiens
106 HDAC4  
Reconstituted Complex Homo sapiens
107 Actb 11461
Affinity Capture-MS Mus musculus
108 CTNNBL1 56259
Affinity Capture-MS Homo sapiens
109 BIN1 274
Reconstituted Complex Homo sapiens
110 PRKCE 5581
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
111 TRIM63  
Biochemical Activity Homo sapiens
Reconstituted Complex Homo sapiens
Co-fractionation Homo sapiens
Two-hybrid Homo sapiens
112 LRCH3 84859
Affinity Capture-MS Homo sapiens
113 HNRNPDL 9987
Co-fractionation Homo sapiens
114 LIMA1 51474
Affinity Capture-MS Homo sapiens
Cross-Linking-MS (XL-MS) Homo sapiens
115 PPP1R1A  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
116 Myh9 17886
Affinity Capture-MS Mus musculus
117 TNNI3K  
Two-hybrid Homo sapiens
118 SMARCD1 6602
Affinity Capture-MS Homo sapiens
119 RC3H2  
Affinity Capture-MS Homo sapiens
120 NCKAP1 10787
Co-fractionation Homo sapiens
121 TRAF3IP1 26146
Affinity Capture-MS Homo sapiens
122 ANXA1 301
Affinity Capture-Western Homo sapiens
123 PRKAR1A 5573
Co-fractionation Homo sapiens
124 ABLIM2  
Co-purification Homo sapiens
125 ACTBL2 345651
Co-fractionation Homo sapiens
Cross-Linking-MS (XL-MS) Homo sapiens
126 BTF3 689
Affinity Capture-MS Homo sapiens
127 MRGBP  
Affinity Capture-MS Homo sapiens
128 MORF4L1  
Affinity Capture-MS Homo sapiens
129 TAGLN 6876
Co-fractionation Homo sapiens
130 MYO1C 4641
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
131 XPO6 23214
Affinity Capture-MS Homo sapiens
132 CDK2 1017
Affinity Capture-MS Homo sapiens
133 ABLIM3 22885
Co-purification Homo sapiens
134 GART 2618
Co-fractionation Homo sapiens
135 ABTB2 25841
Affinity Capture-MS Homo sapiens
136 MYC  
Affinity Capture-MS Homo sapiens
137 PCYT1B 9468
Co-crystal Structure Homo sapiens
138 Apc2  
Co-localization Drosophila melanogaster
139 KAT5  
Affinity Capture-MS Homo sapiens
140 SORBS3 10174
Affinity Capture-MS Homo sapiens
141 MIB2 142678
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
142 MAK16  
Affinity Capture-MS Homo sapiens
143 RHOB 388
Proximity Label-MS Homo sapiens
144 DVL1P1  
Affinity Capture-Western Homo sapiens
145 MECOM 2122
Affinity Capture-MS Homo sapiens
146 AKT1 207
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
147 FSCN1 6624
Reconstituted Complex Homo sapiens
148 TMSB4Y  
Reconstituted Complex Homo sapiens
149 CLDN20  
Affinity Capture-MS Homo sapiens
150 HIF1A 3091
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
151 GSN 2934
Co-crystal Structure Homo sapiens
Co-crystal Structure Homo sapiens
152 DUSP19  
Affinity Capture-MS Homo sapiens
153 RELA 5970
Affinity Capture-MS Homo sapiens
154 TRIP6 7205
Co-localization Homo sapiens
155 SNCA 6622
Reconstituted Complex Homo sapiens
156 POTEJ 653781
Cross-Linking-MS (XL-MS) Homo sapiens
157 STYXL1  
Affinity Capture-MS Homo sapiens
158 SPTAN1 6709
Affinity Capture-Western Homo sapiens
159 HSPB2  
Two-hybrid Homo sapiens
160 USP25  
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
161 FOXRED2  
Affinity Capture-MS Homo sapiens
162 PLEC 5339
Cross-Linking-MS (XL-MS) Homo sapiens
163 POLR1B 84172
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
164 FOLR1 2348
Affinity Capture-MS Homo sapiens
165 CNN1 1264
Reconstituted Complex Homo sapiens
166 SRC 6714
Affinity Capture-Western Homo sapiens
167 CDKN2A 1029
Co-fractionation Homo sapiens
168 DMC1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
169 APC2  
Co-localization Homo sapiens
170 LGALS3 3958
Affinity Capture-MS Homo sapiens
171 Myo1c 17913
Affinity Capture-MS Mus musculus
172 PFDN1 5201
Reconstituted Complex Homo sapiens
173 CAPZA2 830
Affinity Capture-MS Homo sapiens
174 ABL1 25
Reconstituted Complex Homo sapiens
175 MORF4L2  
Affinity Capture-MS Homo sapiens
176 PPP1R9B 84687
Reconstituted Complex Homo sapiens
177 CAP2 10486
Affinity Capture-MS Homo sapiens
178 HDAC1 3065
Affinity Capture-MS Homo sapiens
179 DMAP1 55929
Affinity Capture-MS Homo sapiens
180 TSC1 7248
Co-localization Homo sapiens
181 DNMBP 23268
Affinity Capture-MS Homo sapiens
182 LATS1  
Affinity Capture-MS Homo sapiens
183 Myh10 77579
Affinity Capture-MS Mus musculus
184 MLPH  
Affinity Capture-Western Homo sapiens
185 ACTG1 71
Co-fractionation Homo sapiens
Cross-Linking-MS (XL-MS) Homo sapiens
186 TRIM37  
Proximity Label-MS Homo sapiens
187 CADM4 199731
Affinity Capture-MS Homo sapiens
188 ZNF213  
Affinity Capture-MS Homo sapiens
189 ERRFI1 54206
Affinity Capture-MS Homo sapiens
190 ARRB1 408
Affinity Capture-MS Homo sapiens
191 TRIM31  
Affinity Capture-MS Homo sapiens
192 MAEA 10296
Affinity Capture-MS Homo sapiens
193 CUL7 9820
Affinity Capture-MS Homo sapiens
194 TMSB4X 7114
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
195 C9orf72  
Affinity Capture-MS Homo sapiens
196 RC3H1 149041
Affinity Capture-MS Homo sapiens
197 VIM 7431
Affinity Capture-Western Homo sapiens
198 C19orf80  
Affinity Capture-MS Homo sapiens
199 MECP2 4204
Affinity Capture-MS Homo sapiens
200 EP300 2033
Affinity Capture-MS Homo sapiens
201 CXCL16 58191
Affinity Capture-MS Homo sapiens
202 COTL1 23406
Reconstituted Complex Homo sapiens
View the network image/svg+xml
 Pathways in which ACTA1 is involved
PathwayEvidenceSource
Muscle contraction TAS Reactome
Striated Muscle Contraction TAS Reactome





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