Gene description for SORBS3
Gene name sorbin and SH3 domain containing 3
Gene symbol SORBS3
Other names/aliases SCAM-1
SCAM1
SH3D4
Species Homo sapiens
 Database cross references - SORBS3
ExoCarta ExoCarta_10174
Vesiclepedia VP_10174
Entrez Gene 10174
HGNC 30907
MIM 610795
UniProt O60504  
 SORBS3 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Mesenchymal stem cells 36408942    
Squamous carcinoma cells 20124223    
 Gene ontology annotations for SORBS3
Molecular Function
    structural constituent of cytoskeleton GO:0005200 TAS
    protein binding GO:0005515 IPI
    vinculin binding GO:0017166 IPI
Biological Process
    negative regulation of transcription by RNA polymerase II GO:0000122 IEA
    MAPK cascade GO:0000165 IEA
    cytoskeleton organization GO:0007010 IEA
    cell adhesion GO:0007155 TAS
    cell-substrate adhesion GO:0031589 IBA
    positive regulation of MAPK cascade GO:0043410 IEA
    positive regulation of cytoskeleton organization GO:0051495 NAS
    positive regulation of stress fiber assembly GO:0051496 IDA
Subcellular Localization
    nucleus GO:0005634 IBA
    cytoplasm GO:0005737 IBA
    cytosol GO:0005829 TAS
    cytoskeleton GO:0005856 IEA
    focal adhesion GO:0005925 HDA
    focal adhesion GO:0005925 IDA
    cell-substrate junction GO:0030055 IBA
 Experiment description of studies that identified SORBS3 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 207
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP70|FLOT1
Enriched markers
✔
VDAC
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DKO-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
6
Experiment ID 208
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP70|FLOT1
Enriched markers
✔
VDAC
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name Dks-8
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
7
Experiment ID 209
MISEV standards
✘ Biophysical techniques
✔
TSG101|HSP70|FLOT1
Enriched markers
✔
VDAC
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DLD-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
8
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
9
Experiment ID 191
MISEV standards
✘ Biophysical techniques
✔
Alix|CD81|CD9
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20124223    
Organism Homo sapiens
Experiment description Hypoxic tumor cell modulates its microenvironment to enhance angiogenic and metastatic potential by secretion of proteins and exosomes.
Authors "Park JE, Tan HS, Datta A, Lai RC, Zhang H, Meng W, Lim SK, Sze SK."
Journal name Mol Cell Proteomics
Publication year 2010
Sample Squamous carcinoma cells
Sample name Squamous carcinoma cell (A431)
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for SORBS3
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 TSGA10IP  
Two-hybrid Homo sapiens
2 ENKD1  
Two-hybrid Homo sapiens
3 DLG5 9231
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
4 ABL2  
Affinity Capture-MS Homo sapiens
5 Sgol2  
Affinity Capture-MS Mus musculus
6 CCNH  
Two-hybrid Homo sapiens
7 QARS 5859
Two-hybrid Homo sapiens
8 WIPF2 147179
Two-hybrid Homo sapiens
9 DLGAP2  
Affinity Capture-MS Homo sapiens
10 RIN3  
Affinity Capture-MS Homo sapiens
11 SCNM1  
Two-hybrid Homo sapiens
12 MYO15B 80022
Two-hybrid Homo sapiens
13 ANAPC15  
Affinity Capture-MS Homo sapiens
14 DDX17 10521
Two-hybrid Homo sapiens
15 PAK2 5062
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
16 GAS8  
Two-hybrid Homo sapiens
17 DVL3 1857
Two-hybrid Homo sapiens
18 CCDC102B  
Two-hybrid Homo sapiens
19 ZBTB25  
Two-hybrid Homo sapiens
20 APEX1 328
Affinity Capture-RNA Homo sapiens
21 NOTCH3 4854
Reconstituted Complex Homo sapiens
22 PLOD3 8985
Two-hybrid Homo sapiens
23 CRACR2A  
Two-hybrid Homo sapiens
24 GLRA1  
Two-hybrid Homo sapiens
25 PPFIA3 8541
Two-hybrid Homo sapiens
26 C18orf25 147339
Affinity Capture-MS Homo sapiens
27 AKIRIN2  
Two-hybrid Homo sapiens
28 ADAT3 113179
Two-hybrid Homo sapiens
29 IMP3 55272
Two-hybrid Homo sapiens
30 PPP1R18 170954
Two-hybrid Homo sapiens
31 HGS 9146
Two-hybrid Homo sapiens
32 CPSF7 79869
Two-hybrid Homo sapiens
33 OIP5  
Two-hybrid Homo sapiens
34 MICAL1 64780
Affinity Capture-MS Homo sapiens
35 CPNE1 8904
Two-hybrid Homo sapiens
36 CCDC6 8030
Two-hybrid Homo sapiens
37 TULP3 7289
Affinity Capture-MS Homo sapiens
38 CATSPER1  
Two-hybrid Homo sapiens
39 WASL 8976
Affinity Capture-Western Homo sapiens
40 SETD5 55209
Two-hybrid Homo sapiens
41 DDX6 1656
Two-hybrid Homo sapiens
42 PLEKHN1  
Two-hybrid Homo sapiens
43 PHF1  
Two-hybrid Homo sapiens
44 CBLB 868
Two-hybrid Homo sapiens
45 SORBS3 10174
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
46 ARNT2  
Two-hybrid Homo sapiens
47 DVL2 1856
Two-hybrid Homo sapiens
48 FAM154A  
Two-hybrid Homo sapiens
49 DMRTB1  
Two-hybrid Homo sapiens
50 RBMX 27316
Two-hybrid Homo sapiens
51 MAPK1 5594
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
52 ADAMTSL4  
Two-hybrid Homo sapiens
53 E2F4  
Affinity Capture-MS Homo sapiens
54 KIAA1217 56243
Two-hybrid Homo sapiens
55 CPNE2 221184
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
56 FASLG 356
Protein-peptide Homo sapiens
57 KIFC3 3801
Two-hybrid Homo sapiens
58 UBE2T  
Co-fractionation Homo sapiens
59 PARM1  
Affinity Capture-MS Homo sapiens
60 NCKIPSD 51517
Two-hybrid Homo sapiens
61 TXNDC17 84817
Co-fractionation Homo sapiens
62 SOCS7  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
63 CBL 867
Affinity Capture-Western Homo sapiens
64 PNMA5 114824
Two-hybrid Homo sapiens
65 ACTA1 58
Affinity Capture-MS Homo sapiens
66 CCDC120  
Two-hybrid Homo sapiens
67 GEM  
Two-hybrid Homo sapiens
68 SAFB2 9667
Affinity Capture-Western Homo sapiens
69 CDKN2C  
Two-hybrid Homo sapiens
70 CCNL1  
Two-hybrid Homo sapiens
71 RIN1 9610
Two-hybrid Homo sapiens
72 GYS1 2997
Two-hybrid Homo sapiens
73 SYNCRIP 10492
Affinity Capture-MS Homo sapiens
74 IQUB  
Two-hybrid Homo sapiens
75 CCDC22 28952
Affinity Capture-MS Homo sapiens
76 ALB 213
Affinity Capture-MS Homo sapiens
77 PAK7  
Two-hybrid Homo sapiens
78 C20orf195  
Two-hybrid Homo sapiens
79 COG4 25839
Two-hybrid Homo sapiens
80 TEAD4  
Two-hybrid Homo sapiens
81 C6orf165  
Two-hybrid Homo sapiens
82 VPS37B 79720
Two-hybrid Homo sapiens
83 LRFN4  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
84 BAG3 9531
Affinity Capture-Luminescence Homo sapiens
85 SRCIN1  
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
86 SYNJ1 8867
Affinity Capture-MS Homo sapiens
87 Cdc23  
Affinity Capture-MS Mus musculus
88 PPIL6  
Two-hybrid Homo sapiens
89 OTUD1 220213
Affinity Capture-MS Homo sapiens
90 TBC1D22B  
Two-hybrid Homo sapiens
91 CCDC57 284001
Two-hybrid Homo sapiens
92 ZC4H2 55906
Two-hybrid Homo sapiens
93 DIP2A 23181
Two-hybrid Homo sapiens
94 EFS  
Two-hybrid Homo sapiens
95 VARS2  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
96 HNRNPUL1 11100
Two-hybrid Homo sapiens
97 NAF1  
Two-hybrid Homo sapiens
98 SLC39A1 27173
Affinity Capture-MS Homo sapiens
99 SYP  
Affinity Capture-Western Homo sapiens
100 GPANK1  
Two-hybrid Homo sapiens
101 NRBP1 29959
Two-hybrid Homo sapiens
102 MGC50722  
Two-hybrid Homo sapiens
103 TRIM27  
Two-hybrid Homo sapiens
104 AKT2 208
Two-hybrid Homo sapiens
105 TNS2 23371
Two-hybrid Homo sapiens
106 ZBTB7B  
Two-hybrid Homo sapiens
107 ADORA1  
Affinity Capture-MS Homo sapiens
108 TCEANC  
Two-hybrid Homo sapiens
109 ZC2HC1C  
Two-hybrid Homo sapiens
110 CPNE8 144402
Two-hybrid Homo sapiens
111 ENTHD2  
Two-hybrid Homo sapiens
112 TSGA10  
Two-hybrid Homo sapiens
113 FLOT1 10211
Reconstituted Complex Homo sapiens
114 KIAA1522 57648
Affinity Capture-MS Homo sapiens
115 ARAP1 116985
Affinity Capture-MS Homo sapiens
116 EIF3H 8667
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
117 ATP6V1H 51606
Co-fractionation Homo sapiens
118 ZNF414  
Two-hybrid Homo sapiens
119 KLHL15  
Affinity Capture-MS Homo sapiens
120 FAM53C 51307
Two-hybrid Homo sapiens
121 SYNPO2L  
Two-hybrid Homo sapiens
122 VPS37C 55048
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
123 NEK6 10783
Two-hybrid Homo sapiens
124 Sin3a  
Affinity Capture-MS Mus musculus
125 POLR1C 9533
Two-hybrid Homo sapiens
126 RAD51D  
Two-hybrid Homo sapiens
127 CCHCR1  
Two-hybrid Homo sapiens
128 LINC01420  
Co-fractionation Homo sapiens
129 TNS1 7145
Affinity Capture-MS Homo sapiens
130 MKRN3  
Two-hybrid Homo sapiens
131 FAM109A  
Two-hybrid Homo sapiens
132 UBR3  
Affinity Capture-MS Homo sapiens
133 KATNA1 11104
Affinity Capture-MS Homo sapiens
134 KLHL26  
Affinity Capture-MS Homo sapiens
135 Hdac6  
Affinity Capture-MS Mus musculus
136 OSGIN1  
Two-hybrid Homo sapiens
137 HNRNPK 3190
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
138 NFKBID  
Two-hybrid Homo sapiens
139 ABI2 10152
Two-hybrid Homo sapiens
140 BCL2L13 23786
Affinity Capture-MS Homo sapiens
141 TRIM23 373
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
142 NAB2 4665
Two-hybrid Homo sapiens
143 TRIM41 90933
Two-hybrid Homo sapiens
144 C1orf109  
Two-hybrid Homo sapiens
145 KANK2 25959
Two-hybrid Homo sapiens
146 HNRNPF 3185
Two-hybrid Homo sapiens
147 KRAS 3845
Affinity Capture-MS Homo sapiens
148 SH2D4A 63898
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
149 NHSL2  
Two-hybrid Homo sapiens
150 INSC  
Two-hybrid Homo sapiens
151 MLLT4 4301
Affinity Capture-MS Homo sapiens
152 ZBED1  
Two-hybrid Homo sapiens
153 ANKRD54  
Affinity Capture-MS Homo sapiens
154 KIAA0408  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
155 Cdc26  
Affinity Capture-MS Mus musculus
View the network image/svg+xml
 Pathways in which SORBS3 is involved
PathwayEvidenceSource
Muscle contraction TAS Reactome
Smooth Muscle Contraction TAS Reactome





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