Gene description for SORBS3
Gene name sorbin and SH3 domain containing 3
Gene symbol SORBS3
Other names/aliases SCAM-1
SCAM1
SH3D4
Species Homo sapiens
 Database cross references - SORBS3
ExoCarta ExoCarta_10174
Entrez Gene 10174
HGNC 30907
MIM 610795
UniProt O60504  
 SORBS3 identified in exosomes derived from the following tissue/cell type
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Squamous carcinoma cells 20124223    
 Gene ontology annotations for SORBS3
Molecular Function
    transcription factor binding GO:0008134 IEA
    structural constituent of cytoskeleton GO:0005200 TAS
    protein binding GO:0005515 IPI
    vinculin binding GO:0017166 IPI
Biological Process
    actin filament organization GO:0007015 IEA
    positive regulation of cytoskeleton organization GO:0051495 NAS
    muscle contraction GO:0006936 TAS
    cell-substrate adhesion GO:0031589 IEA
    cell adhesion GO:0007155 TAS
    negative regulation of transcription from RNA polymerase II promoter GO:0000122 IEA
    positive regulation of stress fiber assembly GO:0051496 IDA
    positive regulation of MAPK cascade GO:0043410 IEA
Subcellular Localization
    cytoskeleton GO:0005856 IEA
    focal adhesion GO:0005925 IDA
    cytosol GO:0005829 TAS
    nucleus GO:0005634 IEA
 Experiment description of studies that identified SORBS3 in exosomes
1
Experiment ID 207
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|HSP70
EV Cytosolic markers
✔
FLOT1
EV Membrane markers
✔
VDAC
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ.
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DKO-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
2
Experiment ID 208
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|HSP70
EV Cytosolic markers
✔
FLOT1
EV Membrane markers
✔
VDAC
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ.
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name Dks-8
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 209
ISEV standards
✘ EV Biophysical techniques
✔
TSG101|HSP70
EV Cytosolic markers
✔
FLOT1
EV Membrane markers
✔
VDAC
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ.
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DLD-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 191
ISEV standards
✘ EV Biophysical techniques
✔
Alix
EV Cytosolic markers
✔
CD81|CD9
EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20124223    
Organism Homo sapiens
Experiment description Hypoxic tumor cell modulates its microenvironment to enhance angiogenic and metastatic potential by secretion of proteins and exosomes.
Authors Park JE, Tan HS, Datta A, Lai RC, Zhang H, Meng W, Lim SK, Sze SK.
Journal name Mol Cell Proteomics
Publication year 2010
Sample Squamous carcinoma cells
Sample name Squamous carcinoma cell (A431)
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for SORBS3
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 ZBTB25  
Two-hybrid Homo sapiens
2 HNRNPK 3190
Two-hybrid Homo sapiens
3 KIAA1217 56243
Two-hybrid Homo sapiens
4 KIAA0408  
Two-hybrid Homo sapiens
5 CPSF7 79869
Two-hybrid Homo sapiens
6 TSRC1  
Two-hybrid Homo sapiens
7 DLG5 9231
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
8 DDX17 10521
Two-hybrid Homo sapiens
9 SAFB2  
Affinity Capture-Western Homo sapiens
10 EFS  
Two-hybrid Homo sapiens
11 DVL2 1856
Two-hybrid Homo sapiens
12 CBLB  
Two-hybrid Homo sapiens
13 MAPK1 5594
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
14 DMRTB1  
Two-hybrid Homo sapiens
15 ZBED1  
Two-hybrid Homo sapiens
16 RBMX 27316
Two-hybrid Homo sapiens
View the network image/svg+xml
 Pathways in which SORBS3 is involved
PathwayEvidenceSource
Smooth Muscle Contraction TAS Reactome





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