Gene description for NCKIPSD
Gene name NCK interacting protein with SH3 domain
Gene symbol NCKIPSD
Other names/aliases AF3P21
DIP
DIP1
ORF1
SPIN90
VIP54
WASLBP
WISH
Species Homo sapiens
 Database cross references - NCKIPSD
ExoCarta ExoCarta_51517
Vesiclepedia VP_51517
Entrez Gene 51517
HGNC 15486
MIM 606671
UniProt Q9NZQ3  
 NCKIPSD identified in exosomes derived from the following tissue/cell type
Hepatocytes 26054723    
Mesenchymal stem cells 36408942    
 Gene ontology annotations for NCKIPSD
Molecular Function
    protein binding GO:0005515 IPI
    SH3 domain binding GO:0017124 IEA
    cytoskeletal protein binding GO:0008092 NAS
Biological Process
    Fc-gamma receptor signaling pathway involved in phagocytosis GO:0038096 TAS
    NLS-bearing protein import into nucleus GO:0006607 TAS
    innate immune response GO:0045087 TAS
    cytoskeleton organization GO:0007010 NAS
    signal transduction GO:0007165 TAS
    small GTPase mediated signal transduction GO:0007264 TAS
Subcellular Localization
    cytosol GO:0005829 TAS
    COP9 signalosome GO:0008180 IDA
    intermediate filament GO:0005882 NAS
 Experiment description of studies that identified NCKIPSD in exosomes
1
Experiment ID 237
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocytes
Sample name MIHA
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RNA Sequencing
2
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for NCKIPSD
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 DIAPH3 81624
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
2 NCK2 8440
Two-hybrid Homo sapiens
3 NCK1 4690
Two-hybrid Homo sapiens
4 GRB2 2885
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Invivo Homo sapiens
Invitro Homo sapiens
5 BAIAP2 10458
Two-hybrid Homo sapiens
6 DMRTB1  
Two-hybrid Homo sapiens
View the network image/svg+xml
 Pathways in which NCKIPSD is involved
PathwayEvidenceSource
Regulation of actin dynamics for phagocytic cup formation TAS Reactome
RHO GTPases Activate WASPs and WAVEs TAS Reactome





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