Gene description for Tuba3a
Gene name tubulin, alpha 3A
Gene symbol Tuba3a
Other names/aliases AI528779
M[a]3
Tuba3
Species Mus musculus
 Database cross references - Tuba3a
ExoCarta ExoCarta_22144
Vesiclepedia VP_22144
Entrez Gene 22144
UniProt P05214  
 Tuba3a identified in exosomes derived from the following tissue/cell type
Fibroblasts 23260141    
Oligodendrocytes 21136642    
 Gene ontology annotations for Tuba3a
Molecular Function
    molecular_function GO:0003674 ND
    structural constituent of cytoskeleton GO:0005200 IBA
    GTP binding GO:0005525 IBA
Biological Process
    microtubule cytoskeleton organization GO:0000226 IBA
    mitotic cell cycle GO:0000278 IBA
    male germ-line stem cell population maintenance GO:0036098 IMP
Subcellular Localization
    cytoplasm GO:0005737 IBA
    microtubule GO:0005874 IBA
    microtubule GO:0005874 IDA
    cilium GO:0005929 IDA
    microtubule cytoskeleton GO:0015630 ISO
    ciliary basal body GO:0036064 IDA
 Experiment description of studies that identified Tuba3a in exosomes
1
Experiment ID 210
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|FLOT1
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23260141    
Organism Mus musculus
Experiment description Exosomes Mediate Stromal Mobilization of Autocrine Wnt-PCP Signaling in Breast Cancer Cell Migration.
Authors "Luga V, Zhang L, Viloria-Petit AM, Ogunjimi AA, Inanlou MR, Chiu E, Buchanan M, Hosein AN, Basik M, Wrana JL."
Journal name Cell
Publication year 2012
Sample Fibroblasts
Sample name Normal-Fibroblasts (L cells)
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
2
Experiment ID 33
MISEV standards
✔
EM|IEM
Biophysical techniques
✔
Alix|TSG101
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21136642    
Organism Mus musculus
Experiment description Oligodendrocytes secrete exosomes containing major myelin and stress-protective proteins: Trophic support for axons?
Authors "Kramer-Albers EM, Bretz N, Tenzer S, Winterstein C, Mobius W, Berger H, Nave KA, Schild H, Trotter J"
Journal name PROTEOMICS_CL
Publication year 2007
Sample Oligodendrocytes
Sample name Oligodendrocytes
Oli-neu
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.10-1.14 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QTOF]
Western blotting
Immunoelectron Microscopy
 Protein-protein interactions for Tuba3a
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 SEC22B 9554
Affinity Capture-MS Homo sapiens
2 TUBB 203068
Affinity Capture-MS Homo sapiens
3 HEXB 3074
Affinity Capture-MS Homo sapiens
4 PRDX4 10549
Affinity Capture-MS Homo sapiens
5 OXA1L  
Affinity Capture-MS Homo sapiens
6 SLC25A13 10165
Affinity Capture-MS Homo sapiens
7 CCT7 10574
Affinity Capture-MS Homo sapiens
8 SLC38A2 54407
Affinity Capture-MS Homo sapiens
9 CCT8 10694
Affinity Capture-MS Homo sapiens
10 CCT3 7203
Affinity Capture-MS Homo sapiens
11 REEP5 7905
Affinity Capture-MS Homo sapiens
12 TRPS1  
Affinity Capture-MS Homo sapiens
13 CCT5 22948
Affinity Capture-MS Homo sapiens
14 CTCF  
Affinity Capture-MS Homo sapiens
15 TATDN3  
Affinity Capture-MS Homo sapiens
16 CCHCR1  
Affinity Capture-MS Homo sapiens
17 TYMS 7298
Affinity Capture-MS Homo sapiens
18 CCT2 10576
Affinity Capture-MS Homo sapiens
19 GTF2I 2969
Affinity Capture-MS Homo sapiens
20 DNAJA2 10294
Affinity Capture-MS Homo sapiens
21 CCT6A 908
Affinity Capture-MS Homo sapiens
22 Atxn1  
Proximity Label-MS Mus musculus
Affinity Capture-MS Mus musculus
23 TCP1 6950
Affinity Capture-MS Homo sapiens
24 UNC50  
Affinity Capture-MS Homo sapiens
25 PFDN1 5201
Affinity Capture-MS Homo sapiens
26 SF1 7536
Affinity Capture-MS Homo sapiens
27 TTC3 7267
Affinity Capture-MS Homo sapiens
28 TUBB4B 10383
Affinity Capture-MS Homo sapiens
29 HSP90AB1 3326
Affinity Capture-MS Homo sapiens
30 CCT4 10575
Affinity Capture-MS Homo sapiens
31 PIK3R4 30849
Affinity Capture-MS Homo sapiens
32 TMEM126A 84233
Affinity Capture-MS Homo sapiens
33 FKBP1A 2280
Affinity Capture-MS Homo sapiens
34 HSP90B1 7184
Affinity Capture-MS Homo sapiens
35 TUBA1A 7846
Affinity Capture-MS Homo sapiens
36 TXNDC9 10190
Affinity Capture-MS Homo sapiens
37 HEXA 3073
Affinity Capture-MS Homo sapiens
38 NCKIPSD 51517
Affinity Capture-MS Homo sapiens
39 LGALS3BP 3959
Affinity Capture-MS Homo sapiens
40 GCN1L1 10985
Affinity Capture-MS Homo sapiens
41 PFDN2 5202
Affinity Capture-MS Homo sapiens
42 ASCC1 51008
Affinity Capture-MS Homo sapiens
43 TUBA1B 10376
Affinity Capture-MS Homo sapiens
44 APOL2 23780
Affinity Capture-MS Homo sapiens
45 CTSC 1075
Affinity Capture-MS Homo sapiens
46 SRCAP  
Affinity Capture-MS Homo sapiens
47 ERICH3 127254
Affinity Capture-MS Homo sapiens
48 COL5A1 1289
Affinity Capture-MS Homo sapiens
49 PKP4 8502
Affinity Capture-MS Homo sapiens
50 PIAS2  
Affinity Capture-MS Homo sapiens
51 IFT88 8100
Affinity Capture-MS Homo sapiens
52 BLMH 642
Affinity Capture-MS Homo sapiens
53 DNAJA1 3301
Affinity Capture-MS Homo sapiens
54 SPAST 6683
Affinity Capture-MS Homo sapiens
55 TRIP11 9321
Affinity Capture-MS Homo sapiens
56 TTC9C 283237
Affinity Capture-MS Homo sapiens
57 C11orf30  
Affinity Capture-MS Homo sapiens
58 Snca  
Affinity Capture-Western Mus musculus
Reconstituted Complex Mus musculus
59 INPP5B 3633
Affinity Capture-MS Homo sapiens
60 PLXNA3 55558
Affinity Capture-MS Homo sapiens
61 TRIM29 23650
Affinity Capture-MS Homo sapiens
62 TMEM209 84928
Affinity Capture-MS Homo sapiens
63 RBM33  
Affinity Capture-MS Homo sapiens
64 BCL7A  
Affinity Capture-MS Homo sapiens
65 CRIPT 9419
Affinity Capture-MS Homo sapiens
66 SYNM 23336
Affinity Capture-MS Homo sapiens
67 ESCO1  
Affinity Capture-MS Homo sapiens
68 Fancd2  
Affinity Capture-MS Mus musculus
69 HLA-C 3107
Affinity Capture-MS Homo sapiens
70 LRCH3 84859
Affinity Capture-MS Homo sapiens
71 SPIRE2  
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which Tuba3a is involved
PathwayEvidenceSource
Adaptive Immune System IEA Reactome
Aggrephagy IEA Reactome
Antiviral mechanism by IFN-stimulated genes IEA Reactome
Asparagine N-linked glycosylation IEA Reactome
Autophagy IEA Reactome
Axon guidance IEA Reactome
Carboxyterminal post-translational modifications of tubulin IEA Reactome
Cell Cycle IEA Reactome
Cell Cycle, Mitotic IEA Reactome
Cellular responses to stimuli IEA Reactome
Cellular responses to stress IEA Reactome
Cilium Assembly IEA Reactome
COPI-dependent Golgi-to-ER retrograde traffic IEA Reactome
COPI-independent Golgi-to-ER retrograde traffic IEA Reactome
COPI-mediated anterograde transport IEA Reactome
Cytokine Signaling in Immune system IEA Reactome
Developmental Biology IEA Reactome
EML4 and NUDC in mitotic spindle formation IEA Reactome
ER to Golgi Anterograde Transport IEA Reactome
Factors involved in megakaryocyte development and platelet production IEA Reactome
G2/M Transition IEA Reactome
Gap junction assembly IEA Reactome
Gap junction trafficking IEA Reactome
Gap junction trafficking and regulation IEA Reactome
Golgi-to-ER retrograde transport IEA Reactome
Hedgehog 'off' state IEA Reactome
Hemostasis IEA Reactome
HSP90 chaperone cycle for steroid hormone receptors (SHR) in the presence of ligand IEA Reactome
Immune System IEA Reactome
Interferon Signaling IEA Reactome
Intra-Golgi and retrograde Golgi-to-ER traffic IEA Reactome
Intraflagellar transport IEA Reactome
Kinesins IEA Reactome
L1CAM interactions IEA Reactome
M Phase IEA Reactome
Macroautophagy IEA Reactome
Membrane Trafficking IEA Reactome
Metabolism of proteins IEA Reactome
MHC class II antigen presentation IEA Reactome
Microtubule-dependent trafficking of connexons from Golgi to the plasma membrane IEA Reactome
Mitotic Anaphase IEA Reactome
Mitotic G2-G2/M phases IEA Reactome
Mitotic Metaphase and Anaphase IEA Reactome
Mitotic Prometaphase IEA Reactome
Nervous system development IEA Reactome
Nuclear Envelope (NE) Reassembly IEA Reactome
Organelle biogenesis and maintenance IEA Reactome
PKR-mediated signaling IEA Reactome
Post-translational protein modification IEA Reactome
Recruitment of NuMA to mitotic centrosomes IEA Reactome
Recycling pathway of L1 IEA Reactome
Resolution of Sister Chromatid Cohesion IEA Reactome
RHO GTPase Effectors IEA Reactome
RHO GTPases Activate Formins IEA Reactome
RHO GTPases activate IQGAPs IEA Reactome
Sealing of the nuclear envelope (NE) by ESCRT-III IEA Reactome
Selective autophagy IEA Reactome
Separation of Sister Chromatids IEA Reactome
Signal Transduction IEA Reactome
Signaling by Hedgehog IEA Reactome
Signaling by Rho GTPases IEA Reactome
Signaling by Rho GTPases, Miro GTPases and RHOBTB3 IEA Reactome
The role of GTSE1 in G2/M progression after G2 checkpoint IEA Reactome
Transport of connexons to the plasma membrane IEA Reactome
Transport to the Golgi and subsequent modification IEA Reactome
Vesicle-mediated transport IEA Reactome





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