Gene description for TTC3
Gene name tetratricopeptide repeat domain 3
Gene symbol TTC3
Other names/aliases DCRR1
RNF105
TPRDIII
Species Homo sapiens
 Database cross references - TTC3
ExoCarta ExoCarta_7267
Vesiclepedia VP_7267
Entrez Gene 7267
HGNC 12393
MIM 602259
UniProt P53804  
 TTC3 identified in exosomes derived from the following tissue/cell type
Hepatocellular carcinoma cells 26054723    
Hepatocytes 26054723    
 Gene ontology annotations for TTC3
Molecular Function
    protein binding GO:0005515 IPI
    ligase activity GO:0016874 IEA
    ubiquitin-protein transferase activity GO:0004842 IDA
    zinc ion binding GO:0008270 IEA
Biological Process
    protein K48-linked ubiquitination GO:0070936 IDA
    negative regulation of neuron differentiation GO:0045665 IEA
    ubiquitin-dependent protein catabolic process GO:0006511 IDA
    negative regulation of cell morphogenesis involved in differentiation GO:0010771 IEA
Subcellular Localization
    nucleus GO:0005634 IDA
    mitochondrion GO:0005739 IDA
    vacuole GO:0005773 IEA
    nucleoplasm GO:0005654 IDA
 Experiment description of studies that identified TTC3 in exosomes
1
Experiment ID 235
MISEV standards
EM
EV Biophysical techniques
TSG101|Alix|HSC70|GAPDH
EV Enriched markers
HSP90B1
EV Negative markers
qNano
EV Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
2
Experiment ID 237
MISEV standards
EM
EV Biophysical techniques
TSG101|Alix|HSC70|GAPDH
EV Enriched markers
HSP90B1
EV Negative markers
qNano
EV Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocytes
Sample name MIHA
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RNA Sequencing
 Protein-protein interactions for TTC3
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which TTC3 is involved
No pathways found





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