Gene description for DYNC2LI1
Gene name dynein, cytoplasmic 2, light intermediate chain 1
Gene symbol DYNC2LI1
Other names/aliases CGI-60
D2LIC
LIC3
Species Homo sapiens
 Database cross references - DYNC2LI1
ExoCarta ExoCarta_51626
Vesiclepedia VP_51626
Entrez Gene 51626
HGNC 24595
UniProt Q8TCX1  
 DYNC2LI1 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
 Gene ontology annotations for DYNC2LI1
Molecular Function
    protein binding GO:0005515 IPI
    dynein heavy chain binding GO:0045504 IBA
    dynein heavy chain binding GO:0045504 IDA
Biological Process
    determination of left/right symmetry GO:0007368 IEA
    intraciliary retrograde transport GO:0035721 IBA
    intraciliary transport involved in cilium assembly GO:0035735 IBA
    intraciliary transport involved in cilium assembly GO:0035735 IMP
    regulation of cilium assembly GO:1902017 IMP
Subcellular Localization
    cytoplasm GO:0005737 IDA
    Golgi apparatus GO:0005794 IEA
    centrosome GO:0005813 IDA
    cytosol GO:0005829 IDA
    cytoplasmic dynein complex GO:0005868 IBA
    cytoplasmic dynein complex GO:0005868 IDA
    cytoplasmic dynein complex GO:0005868 ISS
    microtubule GO:0005874 IEA
    cilium GO:0005929 TAS
    axoneme GO:0005930 IBA
    axoneme GO:0005930 ISS
    motile cilium GO:0031514 ISS
    ciliary transition zone GO:0035869 IDA
    ciliary basal body GO:0036064 IDA
    ciliary basal body GO:0036064 ISS
    apical part of cell GO:0045177 ISS
    ciliary tip GO:0097542 TAS
 Experiment description of studies that identified DYNC2LI1 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for DYNC2LI1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 GYPA  
Affinity Capture-MS Homo sapiens
2 Tor1aip1  
Affinity Capture-MS Mus musculus
3 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
4 WDR34 89891
Affinity Capture-MS Homo sapiens
5 ART3  
Affinity Capture-MS Homo sapiens
6 POLR2F  
Affinity Capture-MS Homo sapiens
7 Ercc6l  
Affinity Capture-MS Mus musculus
8 PCYOX1 51449
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
9 LAMP2 3920
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
10 CUL3 8452
Affinity Capture-MS Homo sapiens
11 DYNLT1 6993
Affinity Capture-MS Homo sapiens
12 SSR2  
Affinity Capture-MS Homo sapiens
13 Strip1  
Affinity Capture-MS Mus musculus
14 Wdr5  
Affinity Capture-MS Mus musculus
15 TTC3 7267
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
16 BSG 682
Affinity Capture-MS Homo sapiens
17 Iffo1  
Affinity Capture-MS Mus musculus
18 NPTN 27020
Affinity Capture-MS Homo sapiens
19 CLEC2D  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
20 VRK3 51231
Affinity Capture-MS Homo sapiens
21 PRMT6  
Affinity Capture-MS Homo sapiens
22 ARL14EP  
Affinity Capture-MS Homo sapiens
23 TRIM58 25893
Affinity Capture-MS Homo sapiens
24 CBWD1 55871
Affinity Capture-MS Homo sapiens
25 MAGEA9  
Affinity Capture-MS Homo sapiens
26 SPACA1 81833
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
27 Prpf3 70767
Affinity Capture-MS Mus musculus
28 DYNC1H1 1778
Affinity Capture-MS Homo sapiens
29 TCTEX1D2  
Affinity Capture-MS Homo sapiens
30 TTC19  
Affinity Capture-MS Homo sapiens
31 Cenpf  
Affinity Capture-MS Mus musculus
32 WDR60 55112
Affinity Capture-MS Homo sapiens
33 DCDC2  
Two-hybrid Homo sapiens
34 DYNLRB1 83658
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
35 KIFC1 3833
Affinity Capture-MS Homo sapiens
36 PRKY  
Affinity Capture-MS Homo sapiens
37 CLIP4  
Two-hybrid Homo sapiens
38 G3bp1  
Two-hybrid Mus musculus
39 DYNLL2 140735
Affinity Capture-MS Homo sapiens
40 FAM196A  
Affinity Capture-MS Homo sapiens
41 DYNC2H1 79659
Co-fractionation Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
42 EPHA1 2041
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
43 DYNLL1 8655
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which DYNC2LI1 is involved
PathwayEvidenceSource
Cilium Assembly TAS Reactome
Intraflagellar transport TAS Reactome
Organelle biogenesis and maintenance TAS Reactome





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