Gene description for WDR60
Gene name WD repeat domain 60
Gene symbol WDR60
Other names/aliases FAP163
SRPS6
SRTD8
Species Homo sapiens
 Database cross references - WDR60
ExoCarta ExoCarta_55112
Vesiclepedia VP_55112
Entrez Gene 55112
HGNC 21862
MIM 615462
UniProt Q8WVS4  
 WDR60 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
 Gene ontology annotations for WDR60
Molecular Function
    protein binding GO:0005515 IPI
    dynein light chain binding GO:0045503 IPI
    dynein heavy chain binding GO:0045504 IEA
Biological Process
    intraciliary retrograde transport GO:0035721 IDA
    embryonic skeletal system morphogenesis GO:0048704 IMP
    cilium assembly GO:0060271 IGI
    cilium assembly GO:0060271 IMP
Subcellular Localization
    pericentriolar material GO:0000242 IDA
    spindle pole GO:0000922 IDA
    extracellular space GO:0005615 HDA
    cytoplasm GO:0005737 IEA
    centrosome GO:0005813 IDA
    centrosome GO:0005813 IDA
    cytoplasmic dynein complex GO:0005868 IDA
    cilium GO:0005929 TAS
    interphase microtubule organizing center GO:0031021 IDA
    ciliary tip GO:0097542 TAS
    ciliary base GO:0097546 IDA
    ciliary base GO:0097546 IDA
 Experiment description of studies that identified WDR60 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for WDR60
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 WDFY3 23001
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
2 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
3 Sgol2  
Affinity Capture-MS Mus musculus
4 WDR34 89891
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
5 SIRT6  
Affinity Capture-MS Homo sapiens
6 TK1 7083
Two-hybrid Homo sapiens
7 ZRANB2 9406
Affinity Capture-MS Homo sapiens
8 DYNLT1 6993
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
9 HMGB1 3146
Two-hybrid Homo sapiens
10 DNAJA2 10294
Affinity Capture-MS Homo sapiens
11 TMEM25  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
12 BNIP1 662
Affinity Capture-MS Homo sapiens
13 VSIG8  
Affinity Capture-MS Homo sapiens
14 SPINK14  
Affinity Capture-MS Homo sapiens
15 BPNT1 10380
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
16 SLURP1  
Affinity Capture-MS Homo sapiens
17 Dynlrb1 67068
Affinity Capture-MS Mus musculus
18 C20orf197  
Affinity Capture-MS Homo sapiens
19 FBXW11  
Affinity Capture-MS Homo sapiens
20 TCTEX1D2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
21 NUDC 10726
Affinity Capture-MS Homo sapiens
22 H3F3A 3020
Affinity Capture-MS Homo sapiens
23 ENTPD1 953
Affinity Capture-MS Homo sapiens
24 SRSF7 6432
Affinity Capture-MS Homo sapiens
25 ZCCHC10  
Affinity Capture-MS Homo sapiens
26 DYNLRB1 83658
Proximity Label-MS Homo sapiens
27 FAM46A  
Affinity Capture-MS Homo sapiens
28 TCTEX1D4  
Affinity Capture-MS Homo sapiens
29 Dynll1 56455
Affinity Capture-MS Mus musculus
30 Shoc2  
Affinity Capture-MS Mus musculus
31 MLST8 64223
Affinity Capture-MS Homo sapiens
32 DYNLL2 140735
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
33 H2AFX 3014
Affinity Capture-MS Homo sapiens
34 DYNLRB2 83657
Affinity Capture-MS Homo sapiens
35 GNB2L1 10399
Affinity Capture-MS Homo sapiens
36 DYNLL1 8655
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
37 DDX55  
Affinity Capture-MS Homo sapiens
38 RMDN3 55177
Proximity Label-MS Homo sapiens
39 PLA2G10  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
40 DYNC2LI1 51626
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which WDR60 is involved
PathwayEvidenceSource
Cilium Assembly TAS Reactome
Intraflagellar transport TAS Reactome
Organelle biogenesis and maintenance TAS Reactome





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