Gene description for BNIP1
Gene name BCL2/adenovirus E1B 19kDa interacting protein 1
Gene symbol BNIP1
Other names/aliases NIP1
SEC20
TRG-8
Species Homo sapiens
 Database cross references - BNIP1
ExoCarta ExoCarta_662
Vesiclepedia VP_662
Entrez Gene 662
HGNC 1082
MIM 603291
UniProt Q12981  
 BNIP1 identified in exosomes derived from the following tissue/cell type
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
 Gene ontology annotations for BNIP1
Molecular Function
    protein binding GO:0005515 IPI
    SNAP receptor activity GO:0005484 IDA
Biological Process
    endoplasmic reticulum membrane fusion GO:0016320 IMP
    apoptotic process GO:0006915 IPI
    endoplasmic reticulum organization GO:0007029 IMP
    autophagy GO:0006914 IEA
    negative regulation of apoptotic process GO:0043066 TAS
    vesicle-mediated transport GO:0016192 IEA
    execution phase of apoptosis GO:0097194 IC
Subcellular Localization
    mitochondrion GO:0005739 IEA
    intracellular membrane-bounded organelle GO:0043231 IDA
    SNARE complex GO:0031201 IDA
    nuclear envelope GO:0005635 IDA
    endoplasmic reticulum GO:0005783 IDA
    cytoplasm GO:0005737 IDA
    integral component of endoplasmic reticulum membrane GO:0030176 TAS
 Experiment description of studies that identified BNIP1 in exosomes
1
Experiment ID 834
MISEV standards
✔
EM
EV Biophysical techniques
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
EV Enriched markers
✔
CANX
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 835
MISEV standards
✔
EM
EV Biophysical techniques
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
EV Enriched markers
✔
CANX
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for BNIP1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 BCL2L1 598
Invivo Homo sapiens
Two-hybrid Homo sapiens
2 NCBP1 4686
Two-hybrid Homo sapiens
3 BCL2  
Invitro Homo sapiens
Two-hybrid Homo sapiens
View the network image/svg+xml
 Pathways in which BNIP1 is involved
No pathways found





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