Gene description for BNIP1
Gene name BCL2/adenovirus E1B 19kDa interacting protein 1
Gene symbol BNIP1
Other names/aliases NIP1
SEC20
TRG-8
Species Homo sapiens
 Database cross references - BNIP1
ExoCarta ExoCarta_662
Vesiclepedia VP_662
Entrez Gene 662
HGNC 1082
MIM 603291
UniProt Q12981  
 BNIP1 identified in exosomes derived from the following tissue/cell type
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
 Gene ontology annotations for BNIP1
Molecular Function
    SNAP receptor activity GO:0005484 IDA
    protein binding GO:0005515 IPI
    calcium-induced calcium release activity GO:0048763 IDA
Biological Process
    retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum GO:0006890 IBA
    apoptotic process GO:0006915 IPI
    endoplasmic reticulum organization GO:0007029 IMP
    endoplasmic reticulum membrane fusion GO:0016320 IMP
    calcium-mediated signaling GO:0019722 IEA
    negative regulation of apoptotic process GO:0043066 TAS
    execution phase of apoptosis GO:0097194 IC
    apoptotic process in response to mitochondrial fragmentation GO:0140208 IDA
Subcellular Localization
    nuclear envelope GO:0005635 IDA
    cytoplasm GO:0005737 IDA
    mitochondrial outer membrane GO:0005741 IDA
    endoplasmic reticulum GO:0005783 IBA
    endoplasmic reticulum GO:0005783 IDA
    endoplasmic reticulum membrane GO:0005789 IDA
    endoplasmic reticulum membrane GO:0005789 TAS
    SNARE complex GO:0031201 IBA
    SNARE complex GO:0031201 IDA
    mitochondrial membrane GO:0031966 IDA
    intracellular membrane-bounded organelle GO:0043231 IDA
 Experiment description of studies that identified BNIP1 in exosomes
1
Experiment ID 834
MISEV standards
✔
EM
Biophysical techniques
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 835
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for BNIP1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 SEC22B 9554
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
2 HSD17B11 51170
Two-hybrid Homo sapiens
3 NCBP1 4686
Two-hybrid Homo sapiens
4 BLOC1S6  
Affinity Capture-MS Homo sapiens
5 NAPA 8775
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
6 SRPRB 58477
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
7 NBAS 51594
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
8 TMEM9B 56674
Affinity Capture-MS Homo sapiens
9 DNAJC25 548645
Proximity Label-MS Homo sapiens
10 CLEC12A  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
11 APP 351
Reconstituted Complex Homo sapiens
12 STX1A 6804
Two-hybrid Homo sapiens
13 RINT1 60561
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Cross-Linking-MS (XL-MS) Homo sapiens
14 FAM174A 345757
Two-hybrid Homo sapiens
15 STX8 9482
Affinity Capture-MS Homo sapiens
16 STX7 8417
Affinity Capture-MS Homo sapiens
17 GLT8D2 83468
Affinity Capture-MS Homo sapiens
18 WDR7 23335
Affinity Capture-MS Homo sapiens
19 MARCH5  
Two-hybrid Homo sapiens
20 STX18 53407
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
21 PIGH  
Affinity Capture-MS Homo sapiens
22 C3orf52  
Affinity Capture-MS Homo sapiens
23 CENPQ  
Affinity Capture-MS Homo sapiens
24 CCDC47 57003
Affinity Capture-MS Homo sapiens
25 BET1 10282
Affinity Capture-MS Homo sapiens
26 RIC3  
Affinity Capture-MS Homo sapiens
27 STX12 23673
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
28 TSHR 7253
Two-hybrid Homo sapiens
29 SCFD1 23256
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
30 STOM 2040
Two-hybrid Homo sapiens
31 JAGN1 84522
Two-hybrid Homo sapiens
32 TSPAN3 10099
Affinity Capture-MS Homo sapiens
33 GPR35  
Two-hybrid Homo sapiens
34 CHRNB2  
Affinity Capture-MS Homo sapiens
35 HAUS8  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
36 CCDC107  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
37 TRIM32 22954
Affinity Capture-MS Homo sapiens
38 CREB3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
39 NAPG 8774
Affinity Capture-MS Homo sapiens
40 BCL2L1 598
Reconstituted Complex Homo sapiens
41 VMA21  
Two-hybrid Homo sapiens
42 STX5 6811
Affinity Capture-MS Homo sapiens
43 RNF186  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
44 SCN3B  
Two-hybrid Homo sapiens
45 SEC61B 10952
Proximity Label-MS Homo sapiens
46 CHRND  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
47 SNAP29 9342
Affinity Capture-MS Homo sapiens
48 CXCR4 7852
Affinity Capture-MS Homo sapiens
49 TTYH1  
Affinity Capture-MS Homo sapiens
50 ZDHHC12  
Affinity Capture-MS Homo sapiens
51 TM4SF19  
Two-hybrid Homo sapiens
52 AKAP9 10142
Affinity Capture-MS Homo sapiens
53 LAMP1 3916
Affinity Capture-MS Homo sapiens
54 GOLT1B 51026
Affinity Capture-MS Homo sapiens
55 CRELD1 78987
Affinity Capture-MS Homo sapiens
56 IFT57 55081
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
57 STIM1 6786
Affinity Capture-MS Homo sapiens
58 NIPAL1  
Affinity Capture-MS Homo sapiens
59 LPAR1 1902
Affinity Capture-MS Homo sapiens
60 ZW10 9183
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
61 CHRNB4  
Affinity Capture-MS Homo sapiens
62 FAM134C 162427
Two-hybrid Homo sapiens
63 WDR60 55112
Affinity Capture-MS Homo sapiens
64 B3GNT7  
Affinity Capture-MS Homo sapiens
65 ATP2A1 487
Proximity Label-MS Homo sapiens
66 PTPN1 5770
Proximity Label-MS Homo sapiens
67 CSNK2A2 1459
Affinity Capture-MS Homo sapiens
68 FOCAD 54914
Affinity Capture-MS Homo sapiens
69 ASPH 444
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
70 OTULIN 90268
Affinity Capture-MS Homo sapiens
71 STX16 8675
Affinity Capture-MS Homo sapiens
72 RNF185  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
73 LMNA 4000
Proximity Label-MS Homo sapiens
74 TMX2 51075
Two-hybrid Homo sapiens
75 STX6 10228
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
76 SCFD2 152579
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
77 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
78 USE1 55850
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
79 TMEM9 252839
Affinity Capture-MS Homo sapiens
80 KCNA2  
Affinity Capture-MS Homo sapiens
81 NSF 4905
Affinity Capture-MS Homo sapiens
82 HAUS1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
83 EXOC8 149371
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
84 PGRMC1 10857
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
85 GOSR1 9527
Affinity Capture-MS Homo sapiens
86 TEX28  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
87 Zw10  
Affinity Capture-MS Mus musculus
88 VAMP4 8674
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
89 AGMAT  
Affinity Capture-MS Homo sapiens
90 SQSTM1 8878
Affinity Capture-Western Homo sapiens
91 C19orf25  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
92 GRAMD3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
93 BCL2  
Reconstituted Complex Homo sapiens
94 STX3 6809
Affinity Capture-MS Homo sapiens
95 ZNRF4  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
96 RAB7A 7879
Proximity Label-MS Homo sapiens
97 VAMP5 10791
Affinity Capture-MS Homo sapiens
98 DNAJC1 64215
Proximity Label-MS Homo sapiens
99 TMEM31  
Two-hybrid Homo sapiens
100 ANKRD46 157567
Affinity Capture-MS Homo sapiens
101 CHRNB1  
Affinity Capture-MS Homo sapiens
102 PTGER4  
Two-hybrid Homo sapiens
103 ELOVL4  
Two-hybrid Homo sapiens
104 DERL1 79139
Proximity Label-MS Homo sapiens
View the network image/svg+xml



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