Gene description for ZW10
Gene name zw10 kinetochore protein
Gene symbol ZW10
Other names/aliases HZW10
KNTC1AP
Species Homo sapiens
 Database cross references - ZW10
ExoCarta ExoCarta_9183
Vesiclepedia VP_9183
Entrez Gene 9183
HGNC 13194
MIM 603954
UniProt O43264  
 ZW10 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Chondrocytes 35931686    
Chondrocytes 35931686    
Mesenchymal stem cells 36408942    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
Thymus 23844026    
 Gene ontology annotations for ZW10
Molecular Function
    protein binding GO:0005515 IPI
    centromeric DNA binding GO:0019237 TAS
Biological Process
    mitotic sister chromatid segregation GO:0000070 IDA
    establishment of mitotic spindle orientation GO:0000132 IMP
    endoplasmic reticulum to Golgi vesicle-mediated transport GO:0006888 IBA
    endoplasmic reticulum to Golgi vesicle-mediated transport GO:0006888 IMP
    retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum GO:0006890 NAS
    Golgi organization GO:0007030 IGI
    Golgi organization GO:0007030 IMP
    mitotic metaphase chromosome alignment GO:0007080 IMP
    mitotic spindle assembly checkpoint signaling GO:0007094 IBA
    mitotic spindle assembly checkpoint signaling GO:0007094 IDA
    mitotic spindle assembly checkpoint signaling GO:0007094 IMP
    regulation of exit from mitosis GO:0007096 IDA
    protein transport GO:0015031 IEA
    protein localization to kinetochore GO:0034501 IMP
    cell division GO:0051301 IEA
    meiotic cell cycle GO:0051321 TAS
    regulation of attachment of spindle microtubules to kinetochore GO:0051988 NAS
    protein-containing complex assembly GO:0065003 IDA
Subcellular Localization
    kinetochore GO:0000776 IDA
    spindle pole GO:0000922 IDA
    nucleus GO:0005634 IDA
    endoplasmic reticulum GO:0005783 IDA
    endoplasmic reticulum membrane GO:0005789 IEA
    lipid droplet GO:0005811 IDA
    kinetochore microtubule GO:0005828 IDA
    kinetochore microtubule GO:0005828 NAS
    cytosol GO:0005829 IDA
    cytosol GO:0005829 TAS
    membrane GO:0016020 HDA
    Dsl1/NZR complex GO:0070939 IDA
    Dsl1/NZR complex GO:0070939 IPI
    RZZ complex GO:1990423 IBA
    RZZ complex GO:1990423 IDA
    RZZ complex GO:1990423 IPI
 Experiment description of studies that identified ZW10 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 497
MISEV standards
✘ Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
6
Experiment ID 498
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
7
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
8
Experiment ID 834
MISEV standards
✔
EM
Biophysical techniques
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
9
Experiment ID 835
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
10
Experiment ID 217
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|CD81|CD9|CD63
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors "Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O."
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for ZW10
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 SEC22B 9554
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
2 SCFD1 23256
Affinity Capture-MS Homo sapiens
3 HSD17B11 51170
Proximity Label-MS Homo sapiens
4 SENP3 26168
Affinity Capture-MS Homo sapiens
5 METTL7A 25840
Proximity Label-MS Homo sapiens
6 NAPA 8775
Affinity Capture-MS Homo sapiens
7 HCST  
Affinity Capture-MS Homo sapiens
8 BNIP1 662
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
9 PTF1A  
Two-hybrid Homo sapiens
10 ROCK2 9475
Co-fractionation Homo sapiens
11 PSMD10 5716
Co-fractionation Homo sapiens
12 PTPN1 5770
Proximity Label-MS Homo sapiens
13 STX4 6810
Proximity Label-MS Homo sapiens
14 APP 351
Reconstituted Complex Homo sapiens
15 HSPA4 3308
Co-fractionation Homo sapiens
16 RINT1 60561
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
17 COPB2 9276
Affinity Capture-MS Homo sapiens
18 RPN1 6184
Proximity Label-MS Homo sapiens
19 STOM 2040
Affinity Capture-MS Homo sapiens
20 OPRM1 4988
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
21 ACTR1A 10121
Co-fractionation Homo sapiens
22 SSR1 6745
Proximity Label-MS Homo sapiens
23 STX18 53407
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
24 DGCR2 9993
Affinity Capture-MS Homo sapiens
25 COPA 1314
Affinity Capture-MS Homo sapiens
26 F2RL1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
27 BET1 10282
Affinity Capture-MS Homo sapiens
28 ZWINT  
Affinity Capture-Western Homo sapiens
29 TRAPPC11 60684
Co-fractionation Homo sapiens
30 MAD2L1 4085
Co-localization Homo sapiens
31 GPR17 2840
Affinity Capture-MS Homo sapiens
32 KIAA0196 9897
Co-fractionation Homo sapiens
33 LMAN1 3998
Proximity Label-MS Homo sapiens
34 NUP155 9631
Proximity Label-MS Homo sapiens
35 BSG 682
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
36 LRRC59 55379
Proximity Label-MS Homo sapiens
37 EFNA5  
Affinity Capture-MS Homo sapiens
38 EMD 2010
Proximity Label-MS Homo sapiens
39 COPE 11316
Affinity Capture-MS Homo sapiens
40 PIAS4  
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
41 LDHD  
Affinity Capture-MS Homo sapiens
42 MAD1L1  
Co-localization Homo sapiens
43 ILVBL 10994
Affinity Capture-MS Homo sapiens
44 COMTD1 118881
Affinity Capture-MS Homo sapiens
45 NTRK1 4914
Affinity Capture-MS Homo sapiens
46 GPR182  
Affinity Capture-MS Homo sapiens
47 RAB7A 7879
Proximity Label-MS Homo sapiens
48 ACTB 60
Affinity Capture-MS Homo sapiens
49 ITFG3 83986
Affinity Capture-MS Homo sapiens
50 ELOVL5 60481
Proximity Label-MS Homo sapiens
51 CEBPA  
Protein-peptide Homo sapiens
52 SEC61B 10952
Proximity Label-MS Homo sapiens
53 SLC33A1 9197
Affinity Capture-MS Homo sapiens
54 STX3 6809
Affinity Capture-MS Homo sapiens
55 REEP5 7905
Proximity Label-MS Homo sapiens
56 VIPR2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
57 CYB5B 80777
Affinity Capture-MS Homo sapiens
58 EFNA4  
Affinity Capture-MS Homo sapiens
59 APLNR  
Affinity Capture-MS Homo sapiens
60 SIGLECL1  
Affinity Capture-MS Homo sapiens
61 MSH2 4436
Co-fractionation Homo sapiens
62 UGGT1 56886
Co-fractionation Homo sapiens
63 FZD10  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
64 NPTN 27020
Affinity Capture-MS Homo sapiens
65 CMKLR1 1240
Affinity Capture-MS Homo sapiens
66 GOLT1B 51026
Affinity Capture-MS Homo sapiens
67 TRAF6 7189
Affinity Capture-MS Homo sapiens
68 STIM1 6786
Proximity Label-MS Homo sapiens
69 EGFR 1956
Affinity Capture-MS Homo sapiens
70 METTL21B  
Affinity Capture-MS Homo sapiens
71 FN1 2335
Affinity Capture-MS Homo sapiens
72 OCIAD1 54940
Affinity Capture-MS Homo sapiens
73 C3orf18  
Affinity Capture-MS Homo sapiens
74 RPA3 6119
Proximity Label-MS Homo sapiens
75 ATP2A1 487
Proximity Label-MS Homo sapiens
76 RAB9A 9367
Proximity Label-MS Homo sapiens
77 GSTK1 373156
Affinity Capture-MS Homo sapiens
78 NHP2L1 4809
Co-fractionation Homo sapiens
79 PTPRN  
Affinity Capture-MS Homo sapiens
80 FPR1  
Affinity Capture-MS Homo sapiens
81 SCN2B  
Affinity Capture-MS Homo sapiens
82 HDLBP 3069
Co-fractionation Homo sapiens
83 DDRGK1 65992
Affinity Capture-MS Homo sapiens
84 P2RY10  
Affinity Capture-MS Homo sapiens
85 XRCC3  
Affinity Capture-MS Homo sapiens
86 LMNA 4000
Co-fractionation Homo sapiens
87 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
88 USE1 55850
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
89 STX16 8675
Affinity Capture-MS Homo sapiens
90 SCFD2 152579
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
91 KNTC1 9735
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
92 PGRMC1 10857
Affinity Capture-MS Homo sapiens
93 GOSR1 9527
Affinity Capture-MS Homo sapiens
94 PHLDA3 23612
Affinity Capture-MS Homo sapiens
95 Zw10  
Affinity Capture-MS Mus musculus
96 VAMP4 8674
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
97 CHRM4  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
98 ACTR1B 10120
Co-fractionation Homo sapiens
99 C19orf25  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
100 MFSD4  
Affinity Capture-MS Homo sapiens
101 MKI67  
Affinity Capture-MS Homo sapiens
102 CD80 941
Affinity Capture-MS Homo sapiens
103 CLASP1 23332
Co-fractionation Homo sapiens
104 SEC62 7095
Proximity Label-MS Homo sapiens
105 EEF1D 1936
Co-fractionation Homo sapiens
106 PXN 5829
Proximity Label-MS Homo sapiens
107 VAMP5 10791
Affinity Capture-MS Homo sapiens
108 KIR2DL4  
Affinity Capture-MS Homo sapiens
109 DERL1 79139
Proximity Label-MS Homo sapiens
110 ZWILCH 55055
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
111 EP300 2033
Affinity Capture-MS Homo sapiens
112 S1PR4  
Affinity Capture-MS Homo sapiens
View the network image/svg+xml



Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here