Gene description for UGGT1
Gene name UDP-glucose glycoprotein glucosyltransferase 1
Gene symbol UGGT1
Other names/aliases HUGT1
UGCGL1
UGT1
Species Homo sapiens
 Database cross references - UGGT1
ExoCarta ExoCarta_56886
Entrez Gene 56886
HGNC 15663
MIM 605897
UniProt Q9NYU2  
 UGGT1 identified in exosomes derived from the following tissue/cell type
Hepatocellular carcinoma cells 26054723    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Saliva 19199708    
 Gene ontology annotations for UGGT1
Molecular Function
    UDP-glucose:glycoprotein glucosyltransferase activity GO:0003980 IDA
    unfolded protein binding GO:0051082 IDA
Biological Process
    UDP-glucosylation GO:0097359 IEA
    'de novo' posttranslational protein folding GO:0051084 TAS
    cellular protein metabolic process GO:0044267 TAS
    post-translational protein modification GO:0043687 TAS
    protein N-linked glycosylation via asparagine GO:0018279 TAS
    protein folding GO:0006457 TAS
Subcellular Localization
    endoplasmic reticulum-Golgi intermediate compartment GO:0005793 ISS
    endoplasmic reticulum lumen GO:0005788 TAS
    extracellular exosome GO:0070062 IDA
    endoplasmic reticulum GO:0005783 IDA
 Experiment description of studies that identified UGGT1 in exosomes
1
Experiment ID 234
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
EV Cytosolic markers
✘ EV Membrane markers
✔
HSP90B1
EV Negative markers
✔
qNano
EV Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-C3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
2
Experiment ID 211
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix
EV Cytosolic markers
✔
EpCAM|TFRC
EV Membrane markers
✔
cytochrome c|GOLGA2
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 212
ISEV standards
✔
CEM
EV Biophysical techniques
✔
TSG101|Alix
EV Cytosolic markers
✔
EpCAM|TFRC
EV Membrane markers
✔
Cytochrome C|GOLGA2
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 66
ISEV standards
✔
IEM
EV Biophysical techniques
✔
TSG101|Alix
EV Cytosolic markers
✔
CD63|CD81
EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19199708    
Organism Homo sapiens
Experiment description Proteomic analysis of human parotid gland exosomes by multidimensional protein identification technology (MudPIT).
Authors Gonzalez-Begne M, Lu B, Han X, Hagen FK, Hand AR, Melvin JE, Yates JR
Journal name JPR
Publication year 2009
Sample Saliva
Sample name Saliva
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
Immunoelectron Microscopy
 Protein-protein interactions for UGGT1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 CD40 958
Affinity Capture-MS Homo sapiens
2 WISP1  
Affinity Capture-MS Homo sapiens
3 SUSD2 56241
Affinity Capture-MS Homo sapiens
4 TNFRSF14  
Affinity Capture-MS Homo sapiens
5 CHST12 55501
Affinity Capture-MS Homo sapiens
6 HLA-A  
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which UGGT1 is involved
PathwayEvidenceSource
ER Quality Control Compartment (ERQC) TAS Reactome





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