Gene description for Cct7
Gene name chaperonin containing Tcp1, subunit 7 (eta)
Gene symbol Cct7
Other names/aliases AA408524
AL022769
Ccth
Cctz
Species Mus musculus
 Database cross references - Cct7
ExoCarta ExoCarta_12468
Vesiclepedia VP_12468
Entrez Gene 12468
UniProt P80313  
 Cct7 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
Fibroblasts 23260141    
Mast cells 17486113    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Cct7
Molecular Function
    ATP binding GO:0005524 IEA
    ATP hydrolysis activity GO:0016887 IEA
    protein folding chaperone GO:0044183 ISO
    unfolded protein binding GO:0051082 IBA
    unfolded protein binding GO:0051082 IEA
    ATP-dependent protein folding chaperone GO:0140662 IEA
Biological Process
    protein folding GO:0006457 IBA
    protein folding GO:0006457 ISO
    binding of sperm to zona pellucida GO:0007339 IDA
    positive regulation of telomere maintenance via telomerase GO:0032212 IEA
    positive regulation of telomere maintenance via telomerase GO:0032212 ISO
    protein stabilization GO:0050821 IEA
    protein stabilization GO:0050821 ISO
    chaperone mediated protein folding independent of cofactor GO:0051086 IEA
    chaperone-mediated protein folding GO:0061077 ISO
    positive regulation of establishment of protein localization to telomere GO:1904851 ISO
Subcellular Localization
    mitochondrion GO:0005739 HDA
    chaperonin-containing T-complex GO:0005832 IBA
    chaperonin-containing T-complex GO:0005832 IDA
    chaperonin-containing T-complex GO:0005832 ISO
    microtubule GO:0005874 IEA
    microtubule GO:0005874 ISO
    cell body GO:0044297 IDA
 Experiment description of studies that identified Cct7 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 210
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|FLOT1
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23260141    
Organism Mus musculus
Experiment description Exosomes Mediate Stromal Mobilization of Autocrine Wnt-PCP Signaling in Breast Cancer Cell Migration.
Authors "Luga V, Zhang L, Viloria-Petit AM, Ogunjimi AA, Inanlou MR, Chiu E, Buchanan M, Hosein AN, Basik M, Wrana JL."
Journal name Cell
Publication year 2012
Sample Fibroblasts
Sample name Normal-Fibroblasts (L cells)
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 15
MISEV standards
✔
EM
Biophysical techniques
✔
CD63
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors "Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO"
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
4
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Cct7
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 ACTR2 10097
Affinity Capture-MS Homo sapiens
2 Cct4 12464
Co-fractionation Mus musculus
3 DTL  
Affinity Capture-MS Homo sapiens
4 TUBB 203068
Affinity Capture-MS Homo sapiens
5 CCT8 10694
Affinity Capture-MS Homo sapiens
6 CCT3 7203
Affinity Capture-MS Homo sapiens
7 PDCL3 79031
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
8 Pou5f1  
Affinity Capture-MS Mus musculus
9 UGGT1 56886
Affinity Capture-MS Homo sapiens
10 Tcp1 21454
Co-fractionation Mus musculus
11 CCT2 10576
Affinity Capture-MS Homo sapiens
12 PDCL 5082
Affinity Capture-MS Homo sapiens
13 CCT6A 908
Affinity Capture-MS Homo sapiens
14 WDR92 116143
Affinity Capture-MS Homo sapiens
15 Atxn1  
Proximity Label-MS Mus musculus
16 Kcna3  
Affinity Capture-MS Mus musculus
17 Kctd13  
Affinity Capture-MS Mus musculus
18 Rcc1  
Co-fractionation Mus musculus
19 CCT4 10575
Affinity Capture-MS Homo sapiens
20 HGH1 51236
Affinity Capture-MS Homo sapiens
21 ZNF281  
Affinity Capture-MS Homo sapiens
22 TXNDC9 10190
Affinity Capture-MS Homo sapiens
23 CCT5 22948
Affinity Capture-MS Homo sapiens
24 Tmem173  
Proximity Label-MS Mus musculus
25 Tuba1a 22142
Affinity Capture-Western Mus musculus
26 PDCD5 9141
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
27 TUBA1B 10376
Affinity Capture-MS Homo sapiens
28 Strn3  
Co-fractionation Mus musculus
29 Eed  
Affinity Capture-MS Mus musculus
30 Atg16l1  
Affinity Capture-MS Mus musculus
31 Stk24 223255
Co-fractionation Mus musculus
32 Fancd2  
Affinity Capture-MS Mus musculus
33 TCP1 6950
Affinity Capture-MS Homo sapiens
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