Gene description for CHST8
Gene name carbohydrate (N-acetylgalactosamine 4-0) sulfotransferase 8
Gene symbol CHST8
Other names/aliases GALNAC4ST1
GalNAc4ST
PSS3
Species Homo sapiens
 Database cross references - CHST8
ExoCarta ExoCarta_64377
Vesiclepedia VP_64377
Entrez Gene 64377
HGNC 15993
MIM 610190
UniProt Q9H2A9  
 CHST8 identified in exosomes derived from the following tissue/cell type
Hepatocytes 26054723    
 Gene ontology annotations for CHST8
Molecular Function
    dermatan 4-sulfotransferase GO:0001537 IDA
    dermatan 4-sulfotransferase GO:0001537 TAS
    protein binding GO:0005515 IPI
    sulfotransferase activity GO:0008146 IBA
Biological Process
    sulfur compound metabolic process GO:0006790 IDA
    central nervous system development GO:0007417 NAS
    carbohydrate biosynthetic process GO:0016051 IEA
    proteoglycan biosynthetic process GO:0030166 IBA
    proteoglycan biosynthetic process GO:0030166 IDA
    hormone biosynthetic process GO:0042446 IEP
Subcellular Localization
    Golgi membrane GO:0000139 TAS
    membrane GO:0016020 NAS
 Experiment description of studies that identified CHST8 in exosomes
1
Experiment ID 237
MISEV standards
EM
Biophysical techniques
TSG101|Alix|HSC70|GAPDH
Enriched markers
HSP90B1
Negative markers
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocytes
Sample name MIHA
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RNA Sequencing
 Protein-protein interactions for CHST8
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 DPY19L1 23333
Affinity Capture-MS Homo sapiens
2 APP 351
Affinity Capture-MS Homo sapiens
3 SEL1L 6400
Affinity Capture-MS Homo sapiens
4 TTC13  
Affinity Capture-MS Homo sapiens
5 UGGT1 56886
Affinity Capture-MS Homo sapiens
6 TOR3A 64222
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
7 HLA-DRA 3122
Affinity Capture-MS Homo sapiens
8 SLC30A9 10463
Affinity Capture-MS Homo sapiens
9 CLGN 1047
Affinity Capture-MS Homo sapiens
10 TMED10 10972
Affinity Capture-MS Homo sapiens
11 LRRC1 55227
Affinity Capture-MS Homo sapiens
12 STX10 8677
Affinity Capture-MS Homo sapiens
13 TMBIM6 7009
Affinity Capture-MS Homo sapiens
14 TMED9 54732
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
15 TMEM59 9528
Affinity Capture-MS Homo sapiens
16 P3H4 10609
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
17 CNTNAP1 8506
Affinity Capture-MS Homo sapiens
18 CANX 821
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
19 TMED3 23423
Affinity Capture-MS Homo sapiens
20 JMJD4  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
21 FREM2 341640
Affinity Capture-MS Homo sapiens
22 GAL3ST1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
23 TMED4 222068
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
24 CALU 813
Affinity Capture-MS Homo sapiens
25 B4GALT5 9334
Affinity Capture-MS Homo sapiens
26 PON2 5445
Affinity Capture-MS Homo sapiens
27 SLC39A7 7922
Affinity Capture-MS Homo sapiens
28 MOXD1 26002
Affinity Capture-MS Homo sapiens
29 RRP1 8568
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
30 LMAN2L 81562
Affinity Capture-MS Homo sapiens
31 ERLEC1 27248
Affinity Capture-MS Homo sapiens
32 TMEM160 54958
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
33 FUT8 2530
Affinity Capture-MS Homo sapiens
34 TMX3 54495
Affinity Capture-MS Homo sapiens
35 NOP9 161424
Affinity Capture-MS Homo sapiens
36 APLP2 334
Affinity Capture-MS Homo sapiens
37 OS9 10956
Affinity Capture-MS Homo sapiens
38 RBM27  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
39 CLSTN1 22883
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
40 ERGIC1 57222
Affinity Capture-MS Homo sapiens
41 SPATA20  
Affinity Capture-MS Homo sapiens
42 EDEM3 80267
Affinity Capture-MS Homo sapiens
43 EDEM2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
44 TMED5 50999
Affinity Capture-MS Homo sapiens
45 GALNT12 79695
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
46 PTRH2 51651
Affinity Capture-MS Homo sapiens
47 RBM23  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
48 ERGIC2 51290
Affinity Capture-MS Homo sapiens
49 ARL10  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
50 TAPBP 6892
Affinity Capture-MS Homo sapiens
51 NDUFS8 4728
Affinity Capture-MS Homo sapiens
52 GDF15 9518
Affinity Capture-MS Homo sapiens
53 HLA-DRB3 3125
Affinity Capture-MS Homo sapiens
54 SEC62 7095
Affinity Capture-MS Homo sapiens
55 PAK4 10298
Affinity Capture-MS Homo sapiens
56 FBXO2 26232
Affinity Capture-MS Homo sapiens
57 HSPA5 3309
Affinity Capture-MS Homo sapiens
58 CLSTN3 9746
Affinity Capture-MS Homo sapiens
59 HLA-F 3134
Affinity Capture-MS Homo sapiens
60 GNPTAB 79158
Affinity Capture-MS Homo sapiens
61 CHCHD2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
62 B3GALNT2 148789
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
63 RCN2 5955
Affinity Capture-MS Homo sapiens
64 FRAS1 80144
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
65 C1QBP 708
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
66 TMED2 10959
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
67 LMAN2 10960
Affinity Capture-MS Homo sapiens
68 ADGRG6 57211
Affinity Capture-MS Homo sapiens
69 TXNDC11 51061
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
70 FBL 2091
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
71 LMF1 64788
Affinity Capture-MS Homo sapiens
72 HLA-DRB1 3123
Affinity Capture-MS Homo sapiens
73 UBE2J1 51465
Affinity Capture-MS Homo sapiens
74 YBEY  
Affinity Capture-MS Homo sapiens
75 THEM6  
Affinity Capture-MS Homo sapiens
76 SEPN1 57190
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
77 MRS2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
78 TMED1 11018
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
79 DNAJC16  
Affinity Capture-MS Homo sapiens
80 GPC6 10082
Affinity Capture-MS Homo sapiens
81 FAM3C 10447
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
82 FSTL1 11167
Affinity Capture-MS Homo sapiens
83 MAN1A1 4121
Affinity Capture-MS Homo sapiens
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